التفاصيل البيبلوغرافية
| العنوان: |
Cloning, characterization and gene expression of a metallothionein isoform in the edible cockle Cerastoderma edule after cadmium or mercury exposure. |
| المؤلفون: |
Paul-Pont, Ika, Gonzalez, Patrice, Montero, Natalia, de Montaudouin, Xavier, Baudrimont, Magalie |
| المصدر: |
Ecotoxicology & Environmental Safety; Jan2012, Vol. 75, p119-126, 8p |
| مصطلحات موضوعية: |
GENE expression, CERASTODERMA edule, TRACE elements in animal nutrition, METALLOTHIONEIN, CARRIER proteins, METABOLIC detoxification, PHYSIOLOGICAL effects of cadmium, PHYSIOLOGICAL effects of mercury, NUCLEOTIDE sequence, PHYSIOLOGICAL effects of metals |
| مستخلص: |
Metallothionein (MT) genes encode crucial metal-binding proteins ubiquitously expressed in living organisms and which play important roles in homeostasis of essential metals and detoxification processes. Here, the molecular organization of the first metallothionein gene of the edible cockle Cerastoderma edule and its expression after cadmium (Cd) or mercury (Hg) exposures were determined. The resulting sequence (Cemt1) exhibits unusual features. The full length cDNA encodes a protein of 73 amino acids with nine classical Cys–X(1–3)–Cys motifs, but also one Cys–Cys not generally found in molluscan MT. Moreover, characterization of the molecular organization of the Cemt1 gene revealed two different alleles (A1 and A2) with length differences due to large deletion events in their intronic sequences involving direct Short Interspersed repeated Elements (SINE), while their exonic sequences were identical. To our knowledge, such large excision mechanisms have never before been reported in a bivalve gene sequence. After 10 days of Cd exposure at environmentally relevant doses, quantitative real-time PCR revealed a strong induction of Cemt1 in gills of C. edule. Surprisingly, neither induction of the Cemt1 gene nor of MT protein was shown after Hg exposure, despite the fact that this organism is able to bioaccumulate a high amount of this trace metal which is theoretically one of the most powerful inducers of MT biosynthesis. [Copyright &y& Elsevier] |
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| قاعدة البيانات: |
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