التفاصيل البيبلوغرافية
| العنوان: |
MiR-181a-5p is involved in the cardiomyocytes apoptosis induced by hypoxia–reoxygenation through regulating SIRT1. |
| المؤلفون: |
Qi, Mingxu, He, Li, Ma, Xiaofeng, Li, Zili |
| المصدر: |
Bioscience, Biotechnology & Biochemistry; Jul2020, Vol. 84 Issue 7, p1353-1361, 9p |
| مصطلحات موضوعية: |
APOPTOSIS, SUPEROXIDE dismutase, DNA, LACTATE dehydrogenase, MESSENGER RNA, HYPOXIA-inducible factor 1, POLYVINYLIDENE fluoride |
| مستخلص: |
MiR-181a-5p's mechanism in hypoxia–reoxygenation (H/R)-induced cardiomyocytes apoptosis has not been clarified. This study verified that SIRT1 was the target of miR-181a-5p. MiR-181a-5p expression was up-regulated or down-regulated in H/R-induced cardiomyocytes, and SIRT1 was transfected into cells alone or in combination with miR-181a-5p. Cell viability, apoptosis, levels of released lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD), as well as the Bcl-2, Bax, and Caspase 3 levels in treated cells were tested. On the one hand, down-regulated miR-181a-5p promoted cell viability, reduced released LDH and MDA, and increased SOD level in H/R-induced cardiomyocytes. On the other hand, miR-181a-5p inhibited apoptosis and elevated Bcl-2 expression while decreasing the expressions of Bax and Caspase 3 in treated cells, but the effects of miR-181a-5p could be rescued by SIRT1. In conclusion, miR-181a-5p involved in H/R-induced cardiomyocytes apoptosis through regulating SIRT1, which might become a novel direction for related diseases. SIRT1 counteracted the effects of over-expressed miR-181a-5p on hypoxia/reoxygenation (H/R)-induced cardiomyocytes. H/R: hypoxia–reoxygenation; qRT-PCR: quantitative reverse transcription-polymerase chain reaction; WB: western blot; MDA: malondialdehyde; LDH: lactate dehydrogenase; SOD: superoxide dismutase; AMI: Acute myocardial infarction; I/R: ischemia–reperfusion; DNA: Deoxyribonucleic acid; miRNAs: microRNA; mRNA: messenger RNAs; 3ʹ UTR: 3ʹ-untranslated region; NC: negative control; OD: optical density; PI: propidium iodide; PVDF: polyvinylidene fluoride [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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