دورية
The autophagic pathway is a key component in the lysosomal dependent entry of Trypanosoma cruziinto the host cell
| العنوان: | The autophagic pathway is a key component in the lysosomal dependent entry of Trypanosoma cruziinto the host cell |
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| المؤلفون: | Romano, Patricia S., Arboit, María A., Vázquez, Cristina L., Colombo, María Isabel |
| المصدر: | Autophagy; January 2009, Vol. 5 Issue: 1 p6-18, 13p |
| مستخلص: | The etiologic agent of Chagas disease, Trypanosoma cruzi, infects mammalian cells activating a signal transduction cascade that leads to the formation of its parasitophorous vacuole. Previous works have demonstrated the crucial role of lysosomes in the establishment of T. cruziinfection. In this work we have studied the possible relationship between this parasite and the host cell autophagy. We show, for the first time, that the vacuole containing T. cruzi(TcPV) is decorated by the host cell autophagic protein LC3. Furthermore, live cell imaging experiments indicate that autolysosomes are recruited to parasite entry sites. Interestingly, starvation or pharmacological induction of autophagy before infection significantly increased the number of infected cells whereas inhibitors of this pathway reduced the invasion. In addition, the absence of Atg5 or the reduced expression of Beclin1, two proteins required at the initial steps of autophagosome formation, limited parasite entry and reduced the association between TcPV and the classical lysosomal marker Lamp-1. These results indicate that mammalian autophagy is a key process that favors the colonization of T. cruziin the host cell. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 15548627 15548635 |
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| DOI: | 10.4161/auto.5.1.7160 |