التفاصيل البيبلوغرافية
| العنوان: |
Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics |
| المؤلفون: |
Sitjà-Bobadilla, Ariadna, Gil-Solsona, Rubén, Estensoro, Itziar, Piazzon, M. C, Martos-Sitcha, Juan A, Picard-Sánchez, Amparo, Fuentes, Juan, Sancho, Juan V, Calduch-Giner, Josep A, Hernández, Félix, Pérez-Sánchez, Jaume |
| المساهمون: |
Sapientia |
| بيانات النشر: |
BMC, 2019. |
| سنة النشر: |
2019 |
| مصطلحات موضوعية: |
Myxozoa, Enteromyxum leei, Gilthead sea bream, Teleostei, Aquaculture, Pathophysiology, Tight junctions, Gut barrier, Electrophysiology, Metabolomics |
| الوصف: |
Background In the animal production sector, enteritis is responsible for serious economic losses, and intestinal parasitism is a major stress factor leading to malnutrition and lowered performance and animal production efficiency. The effect of enteric parasites on the gut function of teleost fish, which represent the most ancient bony vertebrates, is far from being understood. The intestinal myxozoan parasite Enteromyxum leei dwells between gut epithelial cells and causes severe enteritis in gilthead sea bream (Sparus aurata), anorexia, cachexia, growth impairment, reduced marketability and increased mortality. Methods This study aimed to outline the gut failure in this fish-parasite model using a multifaceted approach and to find and validate non-lethal serum markers of gut barrier dysfunction. Intestinal integrity was studied in parasitized and non-parasitized fish by immunohistochemistry with specific markers for cellular adhesion (E-cadherin) and tight junctions (Tjp1 and Cldn3) and by functional studies of permeability (oral administration of FITC-dextran) and electrophysiology (Ussing chambers). Serum samples from parasitized and non-parasitized fish were analyzed using non-targeted metabolomics and some significantly altered metabolites were selected to be validated using commercial kits. Results The immunodetection of Tjp1 and Cldn3 was significantly lower in the intestine of parasitized fish, while no strong differences were found in E-cadherin. Parasitized fish showed a significant increase in paracellular uptake measured by FITC-dextran detection in serum. Electrophysiology showed a decrease in transepithelial resistance in infected animals, which showed a diarrheic profile. Serum metabolomics revealed 3702 ions, from which the differential expression of 20 identified compounds significantly separated control from infected groups in multivariate analyses. Of these compounds, serum inosine (decreased) and creatine (increased) were identified as relevant and validated with commercial kits. Conclusions The results demonstrate the disruption of tight junctions and the loss of gut barrier function, a metabolomic profile of absorption dysfunction and anorexia, which further outline the pathophysiological effects of E. leei. |
| الوصف (مترجم): |
This work has been carried out with fnancial support from the European Union under grant projects ParaFishControl (H2020-634429) to ASB and Aquaexcel2020 (652831, TNA AE10004-INTEBREAM) to JF and JPS, and from the Spanish MINECO under AGL2013-48560-R project to ASB and JPS. APS was contracted under the ParaFishControl project, MCP under CSIC PIE project no. 201740E013 and IE under APOSTD/2016/037 grant by the “Generalitat Valen‑ ciana”. Centre for Marine Sciences (CCMAR) is supported by the Portuguese Foundation for Science and Technology (FCT) through project UID/Multi 04326/2019. |
| نوع الوثيقة: |
journal article |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Parasites & Vectors. 2019 Oct 16;12(1):486; 1756-3305; s13071-019-3746-7 |
| الإتاحة: |
http://hdl.handle.net/10400.1/12866Test |
| حقوق: |
open access |
| رقم الانضمام: |
rcaap.com.ualg.sapientia.ualg.pt.10400.1.12866 |
| قاعدة البيانات: |
RCAAP |
