دورية أكاديمية
Validation of a quantitative cerebrospinal fluid alpha-synuclein assay in a European-wide interlaboratory study
| العنوان: | Validation of a quantitative cerebrospinal fluid alpha-synuclein assay in a European-wide interlaboratory study |
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| المؤلفون: | Kruse, Niels, Persson, Staffan, Alcolea, Daniel, Bahl, Justyna M.C., Baldeiras, Ines, Capello, Elisabetta, Chiasserini, Davide, Bocchio Chiavetto, Luisella, Emersic, Andreja, Engelborghs, Sebastiaan, Eren, Erden, Fladby, Tormod, Frisoni, Giovanni, García-Ayllón, María-Salud, Genc, Sermin, Gkatzima, Olymbia, Heegaard, Niels H.H., Janeiro, André M., Kováčech, Branislav, Kuiperij, H. Bea, Leitão, Maria J., Lleó, Alberto, Martins, Madalena, Ramos De Matos, Mafalda, Mollergard, Hanne M., Nobili, Flavio, Öhrfelt, Annika, Parnetti, Lucilla, de Oliveira, Catarina Resende, Rot, Uros, Sáez-Valero, Javier, Struyfs, Hanne, Tanassi, Julia T., Taylor, Peggy, Tsolaki, Magda, Vanmechelen, Eugeen, Verbeek, Marcel M., Zilka, Norbert, Blennow, Kaj, Zetterberg, Henrik, Mollenhauer, Brit |
| المساهمون: | Repositório da Universidade de Lisboa |
| بيانات النشر: | Elsevier, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Alpha-synuclein, Biomarker, Cerebrospinal fluid, ELISA, Validation |
| الوصف: | © 2015 Elsevier Inc. All rights reserved. |
| الوصف (مترجم): | Decreased levels of alpha-synuclein (aSyn) in cerebrospinal fluid (CSF) in Parkinson's disease and related synucleinopathies have been reported, however, not consistently in all cross-sectional studies. To test the performance of one recently released human-specific enzyme-linked immunosorbent assay (ELISA) for the quantification of aSyn in CSF, we carried out a round robin trial with 18 participating laboratories trained in CSF ELISA analyses within the BIOMARKAPD project in the EU Joint Program - Neurodegenerative Disease Research. CSF samples (homogeneous aliquots from pools) and ELISA kits (one lot) were provided centrally and data reported back to one laboratory for data analysis. Our study showed that although factors such as preanalytical sample handling and lot-to-lot variability were minimized by our study design, we identified high variation in absolute values of CSF aSyn even when the same samples and same lots of assays were applied. We further demonstrate that although absolute concentrations differ between laboratories the quantitative results are comparable. With further standardization this assay may become an attractive tool for comparing aSyn measurements in diverse settings. Recommendations for further validation experiments and improvement of the interlaboratory results obtained are given. This is an EU Joint Program – Neurodegenerative Disease Research (JPND) project. The project is supported through the following funding organizations under the aegis of JPND–www.jpnd.eu: The Alzheimer Research Foundation (SAO-FRA), Belgium (grant number S#12018); Danish Council for Strategic Research, Denmark (grant number 0603-00511B); Bundesministerium für Bildung und Forschung (BMBF), Germany (grant number OIED1203C); General Secretary of Research and Technology, Greece; Italian Ministry of Health (Ricerca Corrente), Italy; The Fundação para a Ciência e a Tecnologia, Portugal (grant numbers JPND/0004/2011, IMM/BPD/51-2013, IMM/BI/7-2013, JPND/0005/2011); Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Spain (grant numbers PI11/03026, PI11/03035; cofinanced by the Fondo Europeo de Desarrollo Regional); The Ministry of Education, Research and Sport of the Slovak Republic, Slovakia; The Slovenian Research Agency, Slovenia (grant number A3-0001); The Swedish Research Council, Sweden; Ministry of Education for Netherlands: ZonMw (The Netherlands Organisation for Health Research and Development), The Netherlands (grant number 629000002); The Scientific and Technological Research Council of Turkey (grant number 112S335). |
| نوع الوثيقة: | journal article |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Neurobiol Aging . 2015 Sep;36(9):2587-2596; 0197-4580; 1558-1497 |
| DOI: | 10.1016/j.neurobiolaging.2015.05.003 |
| الإتاحة: | http://hdl.handle.net/10451/53224Test |
| حقوق: | restricted access |
| رقم الانضمام: | rcaap.com.ul.repositorio.ul.pt.10451.53224 |
| قاعدة البيانات: | RCAAP |
| DOI: | 10.1016/j.neurobiolaging.2015.05.003 |
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