التفاصيل البيبلوغرافية
| العنوان: |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo |
| المؤلفون: |
Gouveia, Analuce Canha, Marcelino, Hugo Miguel Rocha, Gonçalves, Lisa, Palmeirim, Isabel, Andrade, Raquel P. |
| المساهمون: |
Universidade do Minho |
| بيانات النشر: |
Taylor & Francis, 2015. |
| سنة النشر: |
2015 |
| مصطلحات موضوعية: |
Animals, Chick Embryo, Chickens, Embryonic Development, Gene Expression Regulation, Developmental, Homeodomain Proteins, In Situ Hybridization, Mesoderm, Hox genes, Hox code, spatial collinearity, temporal collinearity, temporal control, Ciências Médicas::Medicina Básica, Science & Technology |
| الوصف: |
The developing embryo is a paradigmatic model to study molecular mechanisms of time control in Biology. Hox genes are key players in the specification of tissue identity during embryo development and their expression is under strict temporal regulation. However, the molecular mechanisms underlying timely Hox activation in the early embryo remain unknown. This is hindered by the lack of a rigorous temporal framework of sequential Hox expression within a single cluster. Herein, a thorough characterization of HoxB cluster gene expression was performed over time and space in the early chick embryo. Clear temporal collinearity of HoxB cluster gene expression activation was observed. Spatial collinearity of HoxB expression was evidenced in different stages of development and in multiple tissues. Using embryo explant cultures we showed that HoxB2 is cyclically expressed in the rostral presomitic mesoderm with the same periodicity as somite formation, suggesting a link between timely tissue specification and somite formation. We foresee that the molecular framework herein provided will facilitate experimental approaches aimed at identifying the regulatory mechanisms underlying Hox expression in Time and Space. |
| الوصف (مترجم): |
Fundação para a Ciência e a Tecnologia (FCT), Portugal (grants PTDC/SAU-OBD/105111/2008, UMINHO/BI/7/2014 and SFRH/BPD/65652/2009, respectively). RPA was funded by a Ciencia2007 Program Contract and Programa Operacional Regional do Norte (ON.2) – NORTE-07–0124-FEDER-000017. This work was supported by national Portuguese funding through FCT (National and FEDER COMPETE Program funds: PTDC/SAU-BID/121459/2010 and PTDC/SAU-OBD/099758/2008 to RPA and IP, respectively), and by PEst-OE/EQB/LA0023/2011 |
| نوع الوثيقة: |
journal article |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Gouveia, A., Marcelino, H. M., Gonçalves, L., Palmeirim, I., & Andrade, R. P. (2015). Patterning in time and space: HoxB cluster gene expression in the developing chick embryo. Cell Cycle, 14(1), 135-145.; 1538-4101; 1551-4005; 25602523 |
| DOI: |
10.4161/15384101.2014.972868 |
| الإتاحة: |
http://hdl.handle.net/1822/62234Test |
| حقوق: |
open access |
| رقم الانضمام: |
rcaap.com.repositorium.repositorium.sdum.uminho.pt.1822.62234 |
| قاعدة البيانات: |
RCAAP |
