يعرض 1 - 10 نتائج من 277 نتيجة بحث عن '"limited settings"', وقت الاستعلام: 0.72s تنقيح النتائج
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    المساهمون: Intensive Care, MUMC+: MA Medische Staf IC (9), MUMC+: MA Arts Assistenten IC (9), RS: NUTRIM - R2 - Liver and digestive health, Lectoraat Critical Care, Faculteit Gezondheid, Anesthesiology, Graduate School, APH - Quality of Care, Intensive Care Medicine, ACS - Heart failure & arrhythmias, APH - Global Health, ACS - Microcirculation, Nursing, AII - Infectious diseases, AII - Inflammatory diseases, ACS - Pulmonary hypertension & thrombosis, ACS - Diabetes & metabolism, ANS - Neuroinfection & -inflammation, AII - Amsterdam institute for Infection and Immunity, Center of Experimental and Molecular Medicine, Emergency Department, Critical care, Anesthesiology, Peri-operative and Emergency medicine (CAPE), Pulmonary medicine, Intensive care medicine, Group, PRoVENT-COVID Collaborative

    المصدر: The PRoVENT-COVID Collaborative Group, Steering Committee:, Study coordinators: & Investigators: 2022, ' Prognostication using SpO2/FiO2 in invasively ventilated ICU patients with ARDS due to COVID-19 – Insights from the PRoVENT-COVID study ', Journal of Critical Care, vol. 68, pp. 31-37 . https://doi.org/10.1016/j.jcrc.2021.11.009Test
    Journal of Critical Care, 68, 31-37. W B Saunders Co-Elsevier Inc
    Journal of Critical Care, 68, 31-37. Elsevier
    Journal of critical care, 68, 31-37. Elsevier BV
    Journal of Critical Care, 68, 31-37. W B SAUNDERS CO-ELSEVIER INC
    Journal of Critical Care, 68, 31-37. Elsevier BV

    وصف الملف: application/pdf

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    المؤلفون: Monthira Monthatong

    مصطلحات موضوعية: Male-specific marker, RPA, SRY1.Introduction Biological evidence can be used to determine the gender of victims or suspects in forensic cases, including murder, rape and missing persons [1]. Commonly, the morphological and anatomical characteristics of sex organs are used to determine the gender of an unidentified body. Currently, the polymerase chain reaction (PCR) or multiplex PCR techniques are routinely used in the molecular biological process for determining the human gender of organic traces [2]. In forensic study, gender determination was commonly performed using PCR with both the amelogenin (AMEL) and sex-determining region Y (SRY) genes [3]. However, PCR-based assay still has some disadvantages, such as being time-consuming, depending on complicated thermal cycling machines, having a high energy cost, and requiring precise laboratory facilities and highly skilled operators, which restrict the application of PCR in small or unspecialized laboratories and in resource-limited settings. These limitations in PCR-based methods have urged the development of a new molecular biological technique known as isothermal Deoxyribonucleic acid (DNA) amplification that has been extensively reviewed [4-9]. One isothermal DNA amplification technique is called recombinase polymerase amplification (RPA) [10]. RPA is a highly sensitive and selective isothermal amplification technique, performed at a single temperature in the range 37-42?C with minimal sample preparation and is capable of amplifying as low as 1-10 targeted DNA copies in less than 20 min without the need for an initial denaturation step or the use of multiple primers. RPA uses three proteins, including recombinase, single-stranded DNA binding protein (SSB) and strand-displacing polymerase, to amplify double-stranded Deoxyribonucleic acid (dsDNA) in a similar way to the PCR method [11,12]. In the RPA reaction, the recombinases combine with the primers to form recombinase-primer complexes that subsequently scan the double-stranded DNA template for the homologous sequence. The strand displacement reaction is initiated to synthesize new DNA sequences when the primers hybridize to their complementary strand. The displaced template strand is stabilized by SSB and the recombinase dissociates and allows elongation of primers by strand-displacing DNA polymerase. The process generates a complete copy of the

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