نتائج البحث - "Ruminococcaceae"

1

Long-term association between diet quality and characteristics of the gut microbiome in the multiethnic cohort study

المؤلفون: Unhee Lim, V. Wendy Setiawan, Keith R. Curtis, Lynne R. Wilkens, Isaac C. Jenkins, Timothy W. Randolph, Gertraud Maskarinec, Meredith A. J. Hullar, Erica Ma, Johanna W. Lampe, Loic Le Marchand, Carol J. Boushey

المصدر: Br J Nutr

مصطلحات موضوعية: Nutrition and Dietetics, food.ingredient, biology, Ruminococcus, Lachnospiraceae, Medicine (miscellaneous), biology.organism_classification, Coprococcus, Article, food, Anaerostipes, Ruminococcus gnavus, Cohort, Feces, Demography, Ruminococcaceae

الوصف: As past usual diet quality may affect gut microbiome (GM) composition, we examined the association of the Healthy Eating Index (HEI)-2015 assessed 21 and 9 years before stool collection with measures of fecal microbial composition in a subset of the Multiethnic Cohort. A total of 5936 participants completed a validated quantitative FFQ (QFFQ) at cohort entry (Q1, 1993–1996), 5280 at follow-up (Q3, 2003–2008) and 1685 also at a second follow-up (Adiposity Phenotype Study (APS), 2013–2016). All participants provided a stool sample in 2013–2016. Fecal microbial composition was obtained from 16S rRNA gene sequencing (V1–V3 regions). HEI-2015 scores were computed based on each QFFQ. Using linear regression adjusted for relevant covariates, we calculated associations of HEI-2015 scores with gut microbial diversity and 152 individual genera. The mean HEI-2015 scores increased from Q1 (67 (sd 10)) to Q3 (71 (sd 11)) and APS (72 (sd 10)). Alpha diversity assessed by the Shannon Index was significantly higher with increasing tertiles of HEI-2015. Of the 152 bacterial genera tested, seven (Anaerostipes, Coprococcus_2, Eubacterium eligens, Lachnospira, Lachnospiraceae_ND3007, Ruminococcaceae_UCG-013 and Ruminococcus_1) were positively and five (Collinsella, Parabacteroides, Ruminiclostridium_5, Ruminococcus gnavus and Tyzzerella) were inversely associated with HEI-2015 assessed in Q1, Q3 and APS. The estimates of change per unit of the HEI-2015 score associated with the abundance of these twelve genera were consistent across the three questionnaires. The quality of past diet, assessed as far as ∼20 years before stool collection, is equally predictive of GM composition as concurrently assessed diet, indicative of the long-term consistency of this relation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c35eebdf454e9db7fc1ea01676cd214Test
https://pubmed.ncbi.nlm.nih.gov/34369335Test

2

Effects of Starch Overload and Cecal Buffering on Fecal Microbiota of Horses

المؤلفون: Caio C. Bustamante, Vanessa B. de Paula, Isabela P. Rabelo, Camila C. Fernandes, Luciano T. Kishi, Paulo A. Canola, Eliana Gertrudes de M. Lemos, Carlos Augusto A. Valadão

المصدر: Animals; Volume 12; Issue 23; Pages: 3435

مصطلحات موضوعية: General Veterinary, dysbiosis, equine, feces, NSG, Ruminococcaceae, starch overload, Streptococcus, Animal Science and Zoology

الوصف: Starch overload in horses causes gastrointestinal and metabolic disorders that are associated with microbiota changes. Therefore, we identified the fecal microbiota and hypothesized that intracecal injection of alkaline solution (buffer; Mg(OH)2 + Al(OH)3) could stabilize these microbiota and clinical changes in horses submitted to corn starch overload. Ten crossbred horses (females and geldings) were allocated to group I (water–saline and starch–buffer treatments) and group II (water–buffer and starch–saline treatments). Clinical signs, gross analysis of the feces, and fecal microbiota were evaluated through 72 h (T0; T8; T12; T24; T48; T72). Corn starch or water were administrated by nasogastric tube at T0, and the buffer injected into the cecum at T8 in starch–buffer and water–buffer treatments. Starch overload reduced the richness (p < 0.001) and diversity (p = 0.001) of the fecal microbiota. However, the starch–buffer treatment showed greater increase in amylolytic bacteria (Bifidobacterium 0.0% to 5.6%; Lactobacillus 0.1% to 7.4%; p < 0.05) and decrease in fibrolytic bacteria (Lachnospiraceae 10.2% to 5.0%; Ruminococcaceae 11.7% to 4.2%; p < 0.05) than starch–saline treatment. Additionally, animals that received starch–buffer treatment showed more signs of abdominal discomfort and lameness associated with dysbiosis (amylolytic r > 0.5; fribolytic r < 0.1; p < 0.05), showing that cecal infusion of buffer did not prevent, but intensified intestinal disturbances and the risk of laminitis.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19afda463ad26ad6aaacc1b6c2630edbTest

3

Mechanism of Reduction in Allergenicity and Altered Human Intestinal Microbiota of Digested β-Lactoglobulin Modified by Ultrasonic Pretreatment Combined with Glycation

المؤلفون: Yan-hong Shao, Lu Zhang, Jun Liu, Zong-cai Tu, Yao Zhang

المصدر: Journal of Agricultural and Food Chemistry. 69:14004-14012

الوصف: The effects of ultrasound combined with glycation (UCG) on the allergenicity and human microbial community of β-Lg during in vitro digestion were studied by ELISA, cell experiments, and 16S rRNA high-throughput sequencing. UCG modification and subsequent digestion significantly reduced allergenicity. The decrease in the allergenicity of β-Lg depended not only on the low digestibility of glycated β-Lg, which led to the decrease of some peptides with complete immunogenicity, but also the masking effect of glycation on allergen epitopes of β-Lg. Meanwhile, UCG modification and subsequent digestion could alter the structures of intestinal microbiota and the community abundance at phylum, family, and genus levels, such as Bacteroidota, Fusobacteriota, Enterobacteriaceae, Bacteroidaceae, Ruminococcaceae, Bacteroides, and Faecalibacterium. These results show that simulated in vitro digestion of modified β-Lg reduces allergenicity and alters human intestinal microbiota, which could provide a theoretical basis for studying the relationship between intestinal dysbiosis and cow's milk allergy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::682739dd0ca3139d2f2cc7590087c0a4Test
https://doi.org/10.1021/acs.jafc.1c03501Test

4

Poria cocos polysaccharides attenuate chronic nonbacterial prostatitis by targeting the gut microbiota: Comparative study of Poria cocos polysaccharides and finasteride in treating chronic prostatitis

المؤلفون: Guangwen Zhang, Xichun Peng, Liu Liu, Junsheng Liu

المصدر: International Journal of Biological Macromolecules. 189:346-355

الوصف: Finasteride is an antiandrogenic drug used for the clinical treatment of chronic nonbacterial prostatitis (CNP). Recently, we reported the anti-CNP activity of Poria cocos polysaccharides (PPs) in a rat model. In this study, we compared the differences between PPs and finasteride in treating CNP, especially their effects on the gut microbiota. Results showed that both PPs and finasteride significantly reduced the prostate weight and prostate index of CNP rats, and improved the histological damages in the inflamed prostate. Moreover, PPs and finasteride inhibited the production of pro-inflammatory cytokines (TNF-α, IL-2 and IL-8) and androgens (dihydrotestosterone and testosterone). By 16S rDNA sequencing, PPs and finasteride were found to reprogram the gut microbiota into distinct profiles. Further analysis presented that PPs but not finasteride recovered CNP-induced changes in the gut microbiota, including Ruminococcaceae NK4A214 group, uncultured bacterium f Ruminococcaceae, Ruminiclostridium 9, Phascolarctobacterium, Coriobacteriaceae UCG-002 and Oribacterium. LDA effect size (LEfSe) analysis revealed that PPs recovered the gut microbiota by targeting Ruminococcaceae NK4A214 group. Our results suggested that PPs alleviated CNP via different mechanisms from finasteride, especially by regulating the gut microbiota, which offers therapeutic target for the treatment of CNP.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a39b1c0094ad6fac15cc9b9db60f8606Test
https://doi.org/10.1016/j.ijbiomac.2021.08.139Test

5

Association between CD4+ T cell counts and gut microbiota and serum cytokines levels in HIV-infected immunological non-responders

المؤلفون: Yue-Xin Wang, Yi-Qun Kuang, Shi-Tao Geng, Yu Xu, Shaoyou Li, Kunhua Wang, Zunyue Zhang, Danfeng Lu, Jian-Bo Zhang

المصدر: BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-11 (2021)
BMC Infectious Diseases

الوصف: Background CD4+ T cell counts in certain human immunodeficiency virus (HIV)-infected patients called immunological non-responders (INRs) could not return to a normal level even with sustained antiretroviral therapy (ART) because of persistent immune activation, which is associated with pro-inflammatory cytokines production and an altered intestinal microbiome profile. Changes in gut bacterial composition have been linked to low CD4+ T cell counts in HIV-infected individuals. However, the association between CD4+ T cell counts and gut microbiota community composition and cytokines levels in INRs (CD4+ T cell counts Methods To address this issue, we carried out a cross-sectional study of 34 HIV-infected INRs. The patients were divided into CD4 count > 200 cells/μL group and CD4 count 16S rRNA gene sequencing. We also compared CD8+ T cell counts, pro-inflammatory cytokines levels, and nutritional status between the two groups. Results Compared to INRs with CD4 count > 200 cells/μL, those with CD4 count Ruminococcaceae was less abundant in the CD4 count 200 cells/μL group, and difference in alpha diversity was observed between the two groups. Moreover, CD4+ T cell counts were negatively associated with TNF-α and IL-1α levels and positively associated with the relative abundance of Ruminococcaceae. Conclusions Our study demonstrated that lower CD4+ T cell counts in INRs are associated with a reduced abundance of Ruminococcaceae in the gut and elevated serum pro-inflammatory cytokines levels. Thus, interventions targeting gut microbiota to increase CD4+ T cell counts are a potential strategy for promoting immune reconstitution in HIV-infected INRs.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ff6c17ffe5041423c3c685508b32e4cTest
https://doaj.org/article/b623521e3bce456baa25a354a0be20f4Test

6

Amelioration of metabolic disorders by a mushroom-derived polyphenols correlates with the reduction of Ruminococcaceae in gut of DIO mice

المؤلفون: Shanshan Sun, Li Bao, Hongwei Liu, Yangzom Perma, Dorji Phurbu, Shanshan Qiao, Li Sun

المصدر: Food Science and Human Wellness, Vol 10, Iss 4, Pp 442-451 (2021)

الوصف: A polyphenolic alkaloid-enriched extract (PAE) was prepared from the fruiting bodies of a wild edible mushroom Sarcodon leucopus. Oral administration of PAE reduced hyperglycemia, hyperlipidemia, hepatic steatosis, and LPS-related inflammation in high fat diet-induced obese (DIO) mice. Furthermore, we show that PAE produces taxonomic and predicted functional changes in the gut microbiome of DIO mice. A significant decrease in the family of Ruminococcaceae, especially the secondary bile acid-producing bacteria of Intestinimonas and Anaerotruncus, is detected in the gut microbiome of PAE-treate mice. Accordingly, reductions of deoxycholic acid and lithocholic acid are found in the feces of PAE-treated DIO mice, which benefits for the intestinal integrity and the reduction of inflammation. A gut microbiota related mechanism for the anti-metabolic syndrome effects of the PAE is proposed. We suppose the polyphenolic alkaloid extract from S. leucopus be a new and beneficial prebiotic regulating glucose and lipid metabolisms.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed0f0b476888266cd7b0c9d63000bb52Test
http://www.sciencedirect.com/science/article/pii/S2213453021000537Test

7

Relationship between clinical features and intestinal microbiota in Chinese patients with ulcerative colitis

المؤلفون: Jingnan Li, Chu-Yan Chen, Peng-Guang Yan, Ke-Min Li, Xu-Xia He, Xiangchen Meng, Ying-He Li

المصدر: World Journal of Gastroenterology

الوصف: BACKGROUND Dysbacteriosis may be a crucial environmental factor for ulcerative colitis (UC). Further study is required on microbiota alterations in the gastrointestinal tract of patients with UC for better clinical management and treatment. AIM To analyze the relationship between different clinical features and the intestinal microbiota, including bacteria and fungi, in Chinese patients with UC. METHODS Eligible inpatients were enrolled from January 1, 2018 to June 30, 2019, and stool and mucosa samples were collected. UC was diagnosed by endoscopy, pathology, Mayo Score, and Montreal classification. Gene amplicon sequencing of 16S rRNA gene and fungal internal transcribed spacer gene was used to detect the intestinal microbiota composition. Alpha diversity, principal component analysis, similarity analysis, and Metastats analysis were employed to evaluate differences among groups. RESULTS A total of 89 patients with UC and 33 non-inflammatory bowel disease (IBD) controls were enrolled. For bacterial analysis, 72 stool and 48 mucosa samples were obtained from patients with UC and 21 stool and 12 mucosa samples were obtained from the controls. For fungal analysis, stool samples were obtained from 43 patients with UC and 15 controls. A significant difference existed between the fecal and mucosal bacteria of patients with UC. The α-diversity of intestinal bacteria and the relative abundance of some families, such as Lachnospiraceae and Ruminococcaceae, decreased with the increasing severity of bowel inflammation, while Escherichia-Shigella showed the opposite trend. More intermicrobial correlations in UC in remission than in active patients were observed. The bacteria-fungi correlations became single and uneven in patients with UC. CONCLUSION The intestinal bacteria flora of patients with UC differs significantly in terms of various sample types and disease activities. The intermicrobial correlations change in patients with UC compared with non-IBD controls.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf8f6e07e110d63d34d72b10806aa142Test
http://europepmc.org/articles/PMC8326252Test

8

Baicalin-Aluminum Modulates the Broiler Gut Microbiome

المؤلفون: Yinsheng Qiu, Xiaofang Zhang, Dan Zhang, Ling Guo, Yu Liu, Linglu Zhou, Chien-An Andy Hu, Yuzhen Yuan, Shulin Fu, Zhongyuan Wu, Jing He, Chun Ye, Jiacheng Zhang

المصدر: DNA and Cell Biology. 40:881-894

مصطلحات موضوعية: Flavonoids, China, biology, Colistin, Lactobacillus salivarius, Cell Biology, General Medicine, biology.organism_classification, Poultry, Gastrointestinal Microbiome, Microbiology, Metagenomics, Genetics, medicine, Animals, Microbiome, KEGG, Bacteroides fragilis, Chickens, Molecular Biology, Aluminum, Ruminococcaceae, medicine.drug, Bifidobacterium

الوصف: Baicalin-aluminum regulates the gut microbiome of piglets with diarrhea. However, whether it affects poultry gut microbiome composition and function remains unknown. In this study, we used metagenomic sequencing to explore the effects of baicalin-aluminum on gut microbiome changes in poultry when compared with animals administered colistin sulfate. Our data showed that important gut microbiome components consisted of Ruminococcaceae, Subdoligranulum, Bifidobacterium, Bifidobacterium pseudolongum, and Pseudoflavonifractor when broilers were administered baicalin-aluminum compared with colistin. At the species level, Lactobacillus salivarius, Bacteroides uniformis, Oscillibacter unclassified, Bacteroides fragilis, Ruminococcus torques, and Subdoligranulum unclassified abundance were significantly upregulated upon baicalin-aluminum treatment when compared with colistin administration. In addition, Gene Ontology (GO) enrichment analysis indicated that functional differentially expressed genes, which were in the top 30 GO enrichment terms, were associated with metabolic processes, catalytic activity, and cellular processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that ABC transporters, oxidative phosphorylation, and phosphotransferase systems were the dominant signaling pathways in the baicalin-aluminum group when compared with the colistin group. Taken together, our data indicated that baicalin-aluminum modified broiler gut microbiome composition. These observations enhance our physiological insights of baicalin-aluminum-mediated functions in the broiler microbiome and potentially provide a novel therapy to manage both animal and human health.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::779a314316648c356b3e29e4739b78b9Test
https://doi.org/10.1089/dna.2021.0080Test

9

Vitamin D and The Gut Microbiota: a Narrative Literature Review

المؤلفون: Kurosh Djafarian, Hadi Emamat, Hadith Tangestani, Hossein Khosravi Boroujeni, Sakineh Shab-Bidar

المصدر: Clinical Nutrition Research

الوصف: Recently several studies have attempted to investigate the association between vitamin D and microbiota. However, studies have reported inconsistent results. This narrative review aimed to investigate the potential association between vitamin D and microbiota population in the gut by pooling together the results from observational studies and clinical trials. We considered animal and human studies in this field. Several studies have shown the correlation of vitamin D deficiency with microbiota. Furthermore, interventional studies were emerging that vitamin D change the microbiota composition in which leads to an increase in beneficial bacteria, such as Ruminococcaceae, Akkermansia, Faecalibacterium, and Coprococcus while decreases in Firmicutes. Vitamin D could change the microbiota toward decreasing in Firmicutes and increasing in Bacteroidetes. At genera level, vitamin D may connect to some genera of Lachnospiaceae family (e.g., Blautia, Rosburia, Dorea, and Coprococcus). It seems that adequate level of vitamin D is an important factor in improving the composition of the gut microbiota. More studies are needed to confirm possible underling mechanisms.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ea67bd05893593c2e7131eeefdcd6e9Test
http://europepmc.org/articles/PMC8331286Test

10

Comparative study of growth performance, nutrient digestibility, and ruminal and fecal bacterial community between yaks and cattle-yaks raised by stall-feeding

المؤلفون: Rui Hu, Yixiao Zhu, Jian Ma, Guang Cao, Guangyang Li, Lizhi Wang, Qindan Dai, Quanhui Peng, Huawei Zou, Bai Xue, Zhisheng Wang

المصدر: AMB Express, Vol 11, Iss 1, Pp 1-11 (2021)
AMB Express

الوصف: The experiment was conducted to compare the growth performance, rumen fermentation, nutrient digestibility, and ruminal and fecal bacterial community between yaks and cattle-yaks. Ten male yaks (36-month-old) were used as the yak (YAK) group and 10 male cattle-yaks with similar age were selected as the cattle-yak (CAY) group. All the animals were fed same ration and the experiment lasted for 60 days. The results showed that the average daily gain and dry matter intake of CAY group were higher (P P Prevotella 1 and Prevotellaceae UCG-001 were higher (P Succiniclasticum and Butyrivibrio 2 were lower (P the unclassified Lachnospiraceae, Lachnospiraceae NK4A136 group, and Lachnospiraceae AC2044 group were significantly enriched (P Ruminococcaceae UCG-010, Ruminococcaceae UCG-013, and Succiniclasticum were significantly enriched (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98f5966a282bc35db42aadeca4ea228cTest
https://doaj.org/article/bbb26480068c45da9b19122390abe01eTest

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