Recent trends in molecular diagnostics of yeast infections: from PCR to NGS
| العنوان: | Recent trends in molecular diagnostics of yeast infections: from PCR to NGS |
|---|---|
| المؤلفون: | Arastehfar, A, Boekhout, T, Butler, G, De Cesare, G Buda, Dolk, E, Gabaldón, T, Hafez, A, Hube, B, Hagen, F, Hovhannisyan, H, Iracane, E, Kostrzewa, M, Lackner, M, Lass-Flörl, C, Llorens, C, Mixão, V, Munro, C, Oliveira-Pacheco, J, Pekmezovic, M, Pérez-Hansen, A, Sanchez, A Rodriguez, Sauer, F M, Sparbier, K, Stavrou, A A, Vaneechoutte, M, Vatanshenassan, M, Gabaldón, Toni |
| المصدر: | FEMS Microbiology Reviews FEMS MICROBIOLOGY REVIEWS |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Proteomics, Antifungal Agents, diagnosis, Antifungal drugs, Review Article, Drug resistance, Polymerase Chain Reaction, DESORPTION IONIZATION-TIME, Yeast pathogens, CULTURE IDENTIFICATION PANEL, Yeasts, Diagnosis, Medicine and Health Sciences, Sequencing, Pathology, Molecular, Candida, AcademicSubjects/SCI01150, 0303 health sciences, CLINICAL-PRACTICE GUIDELINE, High-Throughput Nucleotide Sequencing, Drug susceptibility, sequencing, 3. Good health, INTERNAL TRANSCRIBED SPACER, Infectious Diseases, Identification (biology), BLOOD-STREAM INFECTIONS, Point-of-Care Systems, Computational biology, Biology, Microbiology, Rapid detection, LASER-DESORPTION/IONIZATION-TIME, 03 medical and health sciences, proteomics, Drug Resistance, Multiple, Fungal, Humans, MALDI-TOF MS, 030304 developmental biology, Whole Genome Sequencing, 030306 microbiology, ESCMID-ASTERISK GUIDELINE, candidemia, Candidemia, Biology and Life Sciences, IN-SITU HYBRIDIZATION, Molecular diagnostics, Yeast, Bench to bedside, Editor's Choice, Mycoses, FLIGHT MASS-SPECTROMETRY, yeast pathogens |
| الوصف: | The incidence of opportunistic yeast infections in humans has been increasing over recent years. These infections are difficult to treat and diagnose, in part due to the large number and broad diversity of species that can underlie the infection. In addition, resistance to one or several antifungal drugs in infecting strains is increasingly being reported, severely limiting therapeutic options and showcasing the need for rapid detection of the infecting agent and its drug susceptibility profile. Current methods for species and resistance identification lack satisfactory sensitivity and specificity, and often require prior culturing of the infecting agent, which delays diagnosis. Recently developed high-throughput technologies such as next generation sequencing or proteomics are opening completely new avenues for more sensitive, accurate and fast diagnosis of yeast pathogens. These approaches are the focus of intensive research, but translation into the clinics requires overcoming important challenges. In this review, we provide an overview of existing and recently emerged approaches that can be used in the identification of yeast pathogens and their drug resistance profiles. Throughout the text we highlight the advantages and disadvantages of each methodology and discuss the most promising developments in their path from bench to bedside. The authors discuss the current status of the use of high-throughput (-omics) technologies on the diagnostics of yeast infections. |
| وصف الملف: | application/pdf |
| تدمد: | 0168-6445 1574-6976 |
| DOI: | 10.1093/femsre/fuz015 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3bcc449d3d9e7c145d5adebdd72b13cTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d3bcc449d3d9e7c145d5adebdd72b13c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01686445 15746976 |
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| DOI: | 10.1093/femsre/fuz015 |