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المؤلفون: Sandeep Sharma, Tarun Kumar, M. John Foulkes, Simon Orford, Anju Mahendru Singh, Luzie U. Wingen, Venkatesh Karnam, Lekshmy S. Nair, Pranab Kumar Mandal, Simon Griffiths, Malcolm J. Hawkesford, Peter R. Shewry, Alison R. Bentley, Renu Pandey
المصدر: CABI Agriculture and Bioscience. 4
مصطلحات موضوعية: Biomass partitioning, Cultural Studies, Grain protein concentration, Grain protein deviation, Triticum aestivum, Religious studies, Nitrogen remobilization efficiency, Low soil nitrogen
الوصف: Background In wheat, nitrogen (N) remobilization from vegetative tissues to developing grains largely depends on genetic and environmental factors. The evaluation of genetic potential of crops under limited resource inputs such as limited N supply would provide an opportunity to identify N-efficient lines with improved N utilisation efficiency and yield potential. We assessed the genetic variation in wheat recombinant inbred lines (RILs) for uptake, partitioning, and remobilization of N towards grain, its association with grain protein concentration (GPC) and grain yield. Methods We used the nested association mapping (NAM) population (195 lines) derived by crossing Paragon (P) with CIMMYT core germplasm (P × Cim), Baj (P × Baj), Watkins (P × Wat), and Wyalkatchem (P × Wya). These lines were evaluated in the field for two seasons under limited N supply. The plant sampling was done at anthesis and physiological maturity stages. Various physiological traits were recorded and total N uptake and other N related indices were calculated. The grain protein deviation (GPD) was calculated from the regression of grain yield on GPC. These lines were grouped into different clusters by hierarchical cluster analysis based on grain yield and N-remobilization efficiency (NRE). Results The genetic variation in accumulation of biomass at both pre- and post-anthesis stages were correlated with grain-yield. The NRE significantly correlated with aboveground N uptake at anthesis (AGNa) and grain yield but negatively associated with AGN at post-anthesis (AGNpa) suggesting higher N uptake till anthesis favours high N remobilization during grain filling. Hierarchical cluster analysis of these RILs based on NRE and yield resulted in four clusters, efficient (31), moderately efficient (59), moderately inefficient (58), and inefficient (47). In the N-efficient lines, AGNa contributed to 77% of total N accumulated in grains, while it was 63% in N-inefficient lines. Several N-efficient lines also exhibited positive grain protein deviation (GPD), combining high grain yield and GPC. Among crosses, the P × Cim were superior and N-efficient, while P × Wya responded poorly to low N input. Conclusions We propose that traits favouring pre- or post-anthesis biomass accumulation and pre-anthesis N uptake may be targeted for breeding to improve grain-yield under limited N. The lines with positive GPD, a first report of genotype-dependent GPD associated with both AGNpa and AGNa in wheat, may be used as varieties or genetic resources to improve grain yield with high GPC for sustainable development under limited N conditions.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50eb17c0eb12045b9efd7e4699692146Test
https://doi.org/10.1186/s43170-023-00153-7Test -
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المؤلفون: Simon Berry, Alba Farre Martinez, Luzie U. Wingen, Sue Freeman, Clare Lister, Simon Griffiths, Jun Ma
المصدر: Journal of Experimental Botany
مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Physiology, QTL, Population, Avalon, Quantitative Trait Loci, Cadenza, Locus (genetics), Plant Science, Quantitative trait locus, Biology, 01 natural sciences, earliness per se, Chromosomes, 03 medical and health sciences, wheat, Genotype, Allele, education, Gene, Triticum, Genetics, photoperiodism, education.field_of_study, flowering, AcademicSubjects/SCI01210, Chromosome, food and beverages, Chromosome Mapping, Vernalization, Bread, yield, Research Papers, 030104 developmental biology, Phenotype, Crop Molecular Genetics, cell wall, 010606 plant biology & botany, height
الوصف: Crop height (Ht), heading date (Hd), and grain yield (GY) are inter-related in wheat. Independent manipulation of each is important for adaptation and performance. Validated quantitative trait loci (QTLs) for all three co-locate on chromosome 3A in the Avalon×Cadenza population, with increased Ht, Hd, and GY contributed by Cadenza. We asked if these are linked or pleiotropic effects using recombinant lines, and showed that Ht and Hd effects are independent. The Chinese Spring equivalent to the newly defined Ht interval contained a gene cluster involved in cell wall growth and displaying high levels of differential transcript expression. The Hd locus is larger and rearranged compared with the reference genome, but FT2 (Flowering Locus T2) is of particular interest. The Hd effect acted independently of photoperiod and vernalization, but did exhibit seasonal genotype×environment interaction. Recombinants were phenotyped for GY in replicated field experiments. GY was most associated with Cadenza alleles for later Hd, supporting physiological studies using the same lines proposing that ‘late’ alleles at this locus increase spike fertility and grain number (GN). The work has uncoupled height from heading and yield, and shown that one of very few validated GY QTLs in wheat is probably mediated by phenological variation.
There are only three validated wheat yield QTLs. Here, one of them was genetically dissected. This showed that the physiological basis of the yield effect is likely to be phenological.الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd226525b3532014dead4e7b0e734fbcTest
http://europepmc.org/articles/PMC8023219Test -
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المؤلفون: Chie Sawada, Carol Moreau, Gabriel H. J. Robinson, Burkhard Steuernagel, Luzie U. Wingen, Jitender Cheema, Ellen Sizer-Coverdale, David Lloyd, Claire Domoney, Noel Ellis
المصدر: Genes; Volume 13; Issue 2; Pages: 196
مصطلحات موضوعية: pea, genetic map, recombinant inbred population, integrated map, Genetics, Genetics (clinical)
الوصف: Biparental recombinant inbred line (RIL) populations are sets of genetically stable lines and have a simple population structure that facilitates the dissection of the genetics of interesting traits. On the other hand, populations derived from multiparent intercrosses combine both greater diversity and higher numbers of recombination events than RILs. Here, we describe a simple population structure: a three-way recombinant inbred population combination. This structure was easy to produce and was a compromise between biparental and multiparent populations. We show that this structure had advantages when analyzing cultivar crosses, and could achieve a mapping resolution of a few genes.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca3d48509af69a98bf43eb0b3bb62361Test
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المؤلفون: Sandeep Sharma, Tarun Kumar, M. John Foulkes, Simon Orford, Anju M. Singh, Luzie U. Wingen, Venkatesh Karnam, Lekshmy S. Nair, Pranab Kumar Mandal, Simon Griffiths, Malcolm J. Hawkesford, Peter R. Shewry, Alison R. Bentley, Renu Pandey
المصدر: SSRN Electronic Journal.
مصطلحات موضوعية: History, Polymers and Plastics, Business and International Management, Industrial and Manufacturing Engineering
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::3287c3a1d230b61a4011e088bfc28d1cTest
https://doi.org/10.2139/ssrn.4002116Test -
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المؤلفون: Chie, Sawada, Carol, Moreau, Gabriel H J, Robinson, Burkhard, Steuernagel, Luzie U, Wingen, Jitender, Cheema, Ellen, Sizer-Coverdale, David, Lloyd, Claire, Domoney, Noel, Ellis
المصدر: Genes. 13(2)
مصطلحات موضوعية: Phenotype, Genetic Linkage, Quantitative Trait Loci, Peas, Chromosome Mapping
الوصف: Biparental recombinant inbred line (RIL) populations are sets of genetically stable lines and have a simple population structure that facilitates the dissection of the genetics of interesting traits. On the other hand, populations derived from multiparent intercrosses combine both greater diversity and higher numbers of recombination events than RILs. Here, we describe a simple population structure: a three-way recombinant inbred population combination. This structure was easy to produce and was a compromise between biparental and multiparent populations. We show that this structure had advantages when analyzing cultivar crosses, and could achieve a mapping resolution of a few genes.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::3d7675f525faec538818d2df8e28793aTest
https://pubmed.ncbi.nlm.nih.gov/35205241Test -
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مصطلحات موضوعية: Agronomy, Specific weight, Yield (finance), Grain quality, Trait, food and beverages, Biology, Adaptation, Falling Number, Genetic architecture, Genetic association
الوصف: There are now a rich variety of genomic and genotypic resources available to wheat researchers and breeders. However, the generation of high-quality and field-relevant phenotyping data which is required to capture the complexities of gene x environment interactions remains a major bottleneck. Historical datasets from national variety performance trials (NVPT) provide sufficient dimensions, in terms of numbers of years and locations, to examine phenotypic trends and study gene x environment interactions. Using NVPT for winter wheat varieties grown in the UK between 2002 – 2017, we examined temporal trends for eight traits related to yield, adaptation, and grain quality performance. We show a non-stationary linear trend for yield, grain protein content, HFN and days to ripening. Our data also show high environmental stability for yield, grain protein content and specific weight in UK winter wheat varieties and high environmental sensitivity for Hagberg Falling Number. Using the historical NVPT data in a genome-wide association analysis, we uncovered a significant marker-trait association peak on wheat chromosome 6A spanning the NAM-A1 gene that have been previously associated with early senescence. Together our results show the value of utilizing the data routinely collected during variety evaluation process for examining breeding progress and the genetic architecture of important traits.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::511a75e8fe7dfcadf2a2574b6926b6daTest
https://doi.org/10.1101/2021.05.17.444481Test -
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المؤلفون: Laura-Jayne Gardiner, Ryan Joynson, Thomas Brabbs, Bernardo J. Clavijo, Neil Hall, Anthony Hall, Jonathan M. Wright, Luzie U. Wingen, James D. Higgins, Simon Griffiths, Paul Bailey
المصدر: Genome Biology, Vol 20, Iss 1, Pp 1-16 (2019)
Genome Biologyمصطلحات موضوعية: 0106 biological sciences, lcsh:QH426-470, QTL, RecQ helicase, Crossover, Quantitative trait locus, Biology, 01 natural sciences, Genome, Chromosomal crossover, Polyploidy, 03 medical and health sciences, 0302 clinical medicine, Crossing Over, Genetic, Gene conversion, Gene, lcsh:QH301-705.5, Triticum, 030304 developmental biology, 2. Zero hunger, Genetics, 0303 health sciences, Genetic diversity, Whole Genome Sequencing, Research, Haplotype, food and beverages, Recombination, lcsh:Genetics, lcsh:Biology (General), Wheat, Genome, Plant, 030217 neurology & neurosurgery, 010606 plant biology & botany
الوصف: Background Sequence exchange between homologous chromosomes through crossing over and gene conversion is highly conserved among eukaryotes, contributing to genome stability and genetic diversity. A lack of recombination limits breeding efforts in crops; therefore, increasing recombination rates can reduce linkage drag and generate new genetic combinations. Results We use computational analysis of 13 recombinant inbred mapping populations to assess crossover and gene conversion frequency in the hexaploid genome of wheat (Triticum aestivum). We observe that high-frequency crossover sites are shared between populations and that closely related parents lead to populations with more similar crossover patterns. We demonstrate that gene conversion is more prevalent and covers more of the genome in wheat than in other plants, making it a critical process in the generation of new haplotypes, particularly in centromeric regions where crossovers are rare. We identify quantitative trait loci for altered gene conversion and crossover frequency and confirm functionality for a novel RecQ helicase gene that belongs to an ancient clade that is missing in some plant lineages including Arabidopsis. Conclusions This is the first gene to be demonstrated to be involved in gene conversion in wheat. Harnessing the RecQ helicase has the potential to break linkage drag utilizing widespread gene conversions. Electronic supplementary material The online version of this article (10.1186/s13059-019-1675-6) contains supplementary material, which is available to authorized users.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c10aa4b1eb5683a38bcd4cdccbcb73aTest
http://link.springer.com/article/10.1186/s13059-019-1675-6Test -
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المؤلفون: Stefano Bencivenga, Luzie U. Wingen, Simon Griffiths, Roxana Savin, Priyanka A. Basavaraddi, Alexandra M. Przewieslik-Allen, Gustavo A. Slafer
المصدر: Repositorio Abierto de la UdL
Universitad de Lleida
Scientific Reports
Recercat. Dipósit de la Recerca de Catalunya
instname
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Crops, Agricultural, Science, media_common.quotation_subject, Quantitative Trait Loci, Fertility, Flowers, Biology, Flowering time, 01 natural sciences, Article, 03 medical and health sciences, Anthesis, Developmental biology, Allele, Alleles, Triticum, media_common, photoperiodism, Analysis of Variance, Multidisciplinary, fungi, food and beverages, Epistasis, Genetic, United Kingdom, Plant Leaves, Horticulture, 030104 developmental biology, Medicine, Allelic Status, Leaf number, Plant sciences, Plant Shoots, 010606 plant biology & botany, Field conditions
الوصف: Earliness per se (Eps) genes are reported to be important in fine-tuning flowering time in wheat independently of photoperiod (Ppd) and vernalisation (Vrn). UnlikePpdandVrngenes,Epshave relatively small effects and their physiological effect along with chromosomal position are not well defined. We evaluated eight lines derived from crossing Paragon and Baj (late and early flowering respectively), vernalisation insensitive, to study the detailed effects of two newly identified QTLs,Eps-7DandEps-2Band their interactions under field conditions. The effect of both QTLs were minor but their effect was modulated by the allelic status of the other. While the magnitude of effect of these QTLs on anthesis was similar, they are associated with very different profiles of pre-anthesis development which also depends on their interaction.Eps-7Daffected both duration before and after terminal spikelet while not affecting final leaf number (FLN) soEps-7D-earlyhad a faster rate of leaf appearance.Eps-2Bacted more specifically in the early reproductive phase and slightly altered FLN without affecting the leaf appearance rate. BothEps-7Dand2Baffected the spike fertility by altering the rate of floret development and mortality. The effect of the latter was very small but consistent in that the -lateallele tended to produced more fertile florets.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77b3d47f9f393e2c7b85ba1d51c1b446Test
http://hdl.handle.net/10459.1/70367Test -
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المؤلفون: Jan Zaucha, Paul A. Wilkinson, Keith J. Edwards, Xingdong Bian, Simon M. Tyrrell, Gary L A Barker, Daniel S. Shaw, Simon Griffiths, Alexandra M. Allen, Amanda J. Burridge, Robert P. Davey, Mark O. Winfield, Luzie U. Wingen
المصدر: Wilkinson, P A, Allen, A M, Tyrrell, S, Wingen, L U, Bian, X, Winfield, M O, Burridge, A, Shaw, D S, Zaucha, J, Griffiths, S, Davey, R P, Edwards, K J & Barker, G L A 2020, ' CerealsDB—new tools for the analysis of the wheat genome : update 2020 ', Database, vol. 2020, baaa060 . https://doi.org/10.1093/database/baaa060Test
Database: The Journal of Biological Databases and Curation
Database: the journal of biological databases and curationمصطلحات موضوعية: 0106 biological sciences, Edible Grain/genetics, Quantitative Trait Loci, Single-nucleotide polymorphism, Computational biology, Biology, Quantitative trait locus, Breeding, Polymorphism, Single Nucleotide, 01 natural sciences, Genome, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Snp markers, Databases, Genetic, Allele, Genotyping, Triticum, Selection (genetic algorithm), 030304 developmental biology, 0303 health sciences, food and beverages, Phenotypic trait, Polymorphism, Single Nucleotide/genetics, Genome, Plant/genetics, Database Update, Triticum/genetics, AcademicSubjects/SCI00960, Edible Grain, General Agricultural and Biological Sciences, Quantitative Trait Loci/genetics, Genome, Plant, Software, 010606 plant biology & botany, Information Systems
الوصف: CerealsDB (www.cerealsdb.uk.net) is an online repository of mainly hexaploid wheat (Triticum aestivum) single nucleotide polymorphisms (SNPs) and genotyping data. The CerealsDB website has been designed to enable wheat breeders and scientists to select the appropriate markers for research breeding tasks, such as marker-assisted selection. We report a large update of genotyping information for over 6000 wheat accessions and describe new webtools for exploring and visualizing the data. We also describe a new database of quantitative trait loci that links phenotypic traits to CerealsDB SNP markers and allelic scores for each of those markers. CerealsDB is an open-access website that hosts information on wheat SNPs considered useful for both plant breeders and research scientists. The latest CerealsDB database is available at https://www.cerealsdb.uk.net/cerealgenomics/CerealsDB/indexNEW.phpTest.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbb17754572b858fe8e71e93481d80f6Test
https://research-information.bris.ac.uk/en/publications/689de7e2-6986-479d-90fd-21410f6dd4ccTest -
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المؤلفون: Alexandra M, Przewieslik-Allen, Paul A, Wilkinson, Amanda J, Burridge, Mark O, Winfield, Xiaoyang, Dai, Mark, Beaumont, Julie, King, Cai-Yun, Yang, Simon, Griffiths, Luzie U, Wingen, Richard, Horsnell, Alison R, Bentley, Peter, Shewry, Gary L A, Barker, Keith J, Edwards
المصدر: Nature plants. 7(2)
مصطلحات موضوعية: Gene Flow, Plant Breeding, Genotype, Chromosomal Instability, Genetic Variation, Bread, Polymorphism, Single Nucleotide, Genome, Plant, Triticum
الوصف: Bread wheat (Triticum aestivum) is one of the world's most important crops; however, a low level of genetic diversity within commercial breeding accessions can significantly limit breeding potential. In contrast, wheat relatives exhibit considerable genetic variation and so potentially provide a valuable source of novel alleles for use in breeding new cultivars. Historically, gene flow between wheat and its relatives may have contributed novel alleles to the bread wheat pangenome. To assess the contribution made by wheat relatives to genetic diversity in bread wheat, we used markers based on single nucleotide polymorphisms to compare bread wheat accessions, created in the past 150 years, with 45 related species. We show that many bread wheat accessions share near-identical haplotype blocks with close relatives of wheat's diploid and tetraploid progenitors, while some show evidence of introgressions from more distant species and structural variation between accessions. Hence, introgressions and chromosomal rearrangements appear to have made a major contribution to genetic diversity in cultivar collections. As gene flow from relatives to bread wheat is an ongoing process, we assess the impact that introgressions might have on future breeding strategies.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::1e8aa7e71961c68cc5882345d4d78ac9Test
https://pubmed.ncbi.nlm.nih.gov/33526912Test
