Induction of Apoptosis by Three Marine Algae through Generation of Reactive Oxygen Species in Human Leukemic Cell Lines
| العنوان: | Induction of Apoptosis by Three Marine Algae through Generation of Reactive Oxygen Species in Human Leukemic Cell Lines |
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| المؤلفون: | Huey-Lan Huang, Shwu-Li Wu, Ray-Ling Huang, Hui-Fen Liao, Yuh-Cheng Yang, Yu-Jen Chen, Chii-Ming Jiang, Yu-Yawn Chen |
| المصدر: | Journal of Agricultural and Food Chemistry. 53:1776-1781 |
| بيانات النشر: | American Chemical Society (ACS), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Carcinoma, Hepatocellular, Apoptosis, HL-60 Cells, Biology, Phaeophyta, Flow cytometry, chemistry.chemical_compound, Chlorophyta, Tumor Cells, Cultured, medicine, Humans, Colpomenia sinuosa, chemistry.chemical_classification, Reactive oxygen species, U937 cell, medicine.diagnostic_test, Superoxide, Liver Neoplasms, Eukaryota, General Chemistry, Molecular biology, chemistry, Biochemistry, Leukemia, Myeloid, Cell culture, Rhodophyta, Apoptotic signaling pathway, Reactive Oxygen Species, General Agricultural and Biological Sciences |
| الوصف: | In this study, we examined the antitumor effect of marine algae extracts on human hepatoma and leukemia cells. Ethyl acetate extracts from Colpomenia sinuosa (Cs-EA), Halimeda discoidae (Hd-EA), and Galaxaura oblongata (Go-EA) directly inhibited the growth of human hepatoma HuH-7 cells and leukemia U937 and HL-60 cells in a time- and dose-dependent manner. Specifically, these algae extracts induced apoptosis of U937 and HL-60 cells as evaluated by detection of hypodiploid cells using flow cytometry and observation of condensed and fragmented nuclei in algae extract-treated cells. Intracellular reactive oxygen species (ROS), especially hydrogen peroxide and superoxide anion, were increased about 2-3-fold in U937 cells treated with Cs-EA for 3-5 h. Interestingly, antioxidant N-acetylcysteine effectively blocked Cs-EA-, Hd-EA-, and Go-EA-induced apoptosis, suggesting that ROS is a key mediator in the apoptotic signaling pathway. In conclusion, our results show that algae extracts induce apoptosis in human leukemia cells through generation of ROS. |
| تدمد: | 1520-5118 0021-8561 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2701728978c9c98ca03561974e8740cdTest https://doi.org/10.1021/jf049445nTest |
| رقم الانضمام: | edsair.doi.dedup.....2701728978c9c98ca03561974e8740cd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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