Cancer stem cells are enriched in Fanconi anemia head and neck squamous cell carcinomas

التفاصيل البيبلوغرافية
العنوان: Cancer stem cells are enriched in Fanconi anemia head and neck squamous cell carcinomas
المؤلفون: Ananth Annapragada, Nadarajah Vigneswaran, Varatharasa Thiviyanathan, Jean Wu, Qingshan Mu
المصدر: International Journal of Oncology
بيانات النشر: Spandidos Publications, 2014.
سنة النشر: 2014
مصطلحات موضوعية: cancer stem cells, Cancer Research, Pathology, Chromosomal Proteins, Non-Histone, medicine.disease_cause, Metastasis, Mice, education.field_of_study, Nanog Homeobox Protein, Articles, Middle Aged, Cell cycle, 3. Good health, Gene Expression Regulation, Neoplastic, Isoenzymes, Oncology, Head and Neck Neoplasms, Carcinoma, Squamous Cell, Neoplastic Stem Cells, Female, Homeobox protein NANOG, medicine.medical_specialty, tumorspheres, Population, Biology, head and neck squamous cell carcinoma, Aldehyde Dehydrogenase 1 Family, stomatognathic system, Cancer stem cell, otorhinolaryngologic diseases, medicine, Animals, Humans, Aldefluor assay, xenograft, education, neoplasms, Homeodomain Proteins, Squamous Cell Carcinoma of Head and Neck, Proteins, Retinal Dehydrogenase, Cancer, medicine.disease, Xenograft Model Antitumor Assays, Head and neck squamous-cell carcinoma, stomatognathic diseases, Fanconi Anemia, Neoplasm Recurrence, Local, Carcinogenesis, Octamer Transcription Factor-3
الوصف: Fanconi anemia (FA) patients have an increased risk of head and neck squamous cell carcinoma (HNSCC) at a higher rate with no apparent risk factors. HNSCC of FA patients is an aggressive tumor characterized by multifocal origin, early metastases and frequent recurrences. Given that cancer stem cells (CSC) drive tumorigenesis, tumor recurrence and metastasis, in this study, we characterized the CSC population in FA and sporadic HNSCC. The Aldefluor assay was used to characterize and isolate CSC with high aldehyde dehydrogenase (ALDH) activity (ALDHpos) in cell lines derived from FA and sporadic HNSCC. Isolated ALDHpos and ALDHneg cells were examined for the expression of stemness genes using reverse transcription-polymerase chain reaction (RT-PCR) array. Tumor cell-derived FA and sporadic HNSCC were examined for their ability to form tumorspheres in vitro. Stem-like cell population in FA and sporadic HNSCC in human and mouse xenograft tumors were evaluated using ALDH isoform 1 (ALDH1) immunohistochemistry. FA-HNSCC cell lines harbor a greater proportion of ALDHpos cells (15–31%) compared to sporadic HNSCC (10%). Expression of Nanog, Oct-3/4 and Stella, molecular markers of undifferentiated embryonic stem (ES) cells were detected in the ALDHpos FA-HNSCC cells and not in the ALDHneg cells. FA-HNSCC cell lines revealed enhanced in vitro tumorsphere formation compared to sporadic HNSCC cells. A higher percentage of ALDH1pos tumor cells are noted in the human and mouse xenograft tumors of FA-HNSCC compared to sporadic HNSCC tumors. FA-HNSCC are highly enriched for CSC and may serve as a model to develop CSC-targeted therapies for HNSCC.
تدمد: 1791-2423
1019-6439
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ec437e7535c4dc0a65372ff15859dccTest
https://doi.org/10.3892/ijo.2014.2677Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....2ec437e7535c4dc0a65372ff15859dcc
قاعدة البيانات: OpenAIRE