Identification of cancer-type specific expression patterns for active aldehyde dehydrogenase (ALDH) isoforms in ALDEFLUOR assay
| العنوان: | Identification of cancer-type specific expression patterns for active aldehyde dehydrogenase (ALDH) isoforms in ALDEFLUOR assay |
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| المؤلفون: | Suling Liu, Qiaodan Deng, Lei Zhou, Dong Wang, Wei Ma, Lu Deng, Dandan Sheng |
| المصدر: | Cell Biology and Toxicology |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Gene isoform, Short Communication, Health, Toxicology and Mutagenesis, Population, Aldehyde dehydrogenase, Toxicology, Cell Line, Aldehyde dehydrogenase (ALDH), Immunoenzyme Techniques, 03 medical and health sciences, 0302 clinical medicine, Cancer stem cell, Neoplasms, ALDEFLUOR assay, medicine, Humans, BAAA, DEAB, education, education.field_of_study, biology, Genome, Human, Chemistry, Gene Expression Profiling, Cancer, Cell Biology, Aldehyde Dehydrogenase, medicine.disease, Enzyme assay, Isoenzymes, ALDH1A1, HEK293 Cells, 030104 developmental biology, Biochemistry, 030220 oncology & carcinogenesis, Neoplastic Stem Cells, Cancer stem cell (CSC), biology.protein, Stem cell |
| الوصف: | Aldehyde dehydrogenases (ALDHs) defend intracellular homeostasis by catalyzing the conversion of toxic aldehydes into non-toxic carboxylic acids, which is of particular importance to the self-renewal of stem cells and cancer stem cells. The widely used ALDEFLUOR assay was initially designed to indicate the activity of ALDH1A1 in leukemia and has been demonstrated to detect the enzyme activity of several other ALDH isoforms in various cancer types in recent years. However, it is still elusive which isoforms, among the 19 ALDH isoforms in human genome, are the potential contributors in catalyzing ALDEFLUOR assay in different cancers. In the current study, we performed a screening via overexpressing each ALDH isoform to assess their ability of catalyzing ALDEFLUOR assay. Our results demonstrate that nine isoforms are active in ALDEFLUOR assay, whose overexpression significantly increases ALDH-positive (ALDH+) population. Further analysis of the expression of these active isoforms in various cancers reveals cancer-type specific expression patterns, suggesting that different cancer types may exhibit ALDEFLUOR activity through expression of specific active ALDH isoforms. This study strongly indicates that a detailed elucidation of the functions for each active ALDH isoform in CSCs is necessary and important for a profound understanding of the underlying mechanisms of ALDH-associated stemness. Electronic supplementary material The online version of this article (10.1007/s10565-018-9444-y) contains supplementary material, which is available to authorized users. |
| تدمد: | 1573-6822 0742-2091 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba6d92578447c48b2bf00e0b9f3c9c38Test https://doi.org/10.1007/s10565-018-9444-yTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ba6d92578447c48b2bf00e0b9f3c9c38 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15736822 07422091 |
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