Maternal and Infant Immune Repertoire Sequencing Analysis Identifies Distinct Ig and TCR Development in Term and Preterm Infants
| العنوان: | Maternal and Infant Immune Repertoire Sequencing Analysis Identifies Distinct Ig and TCR Development in Term and Preterm Infants |
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| المؤلفون: | Marina Sirota, Ji-Yeun Lee, Quoc-Hung Nguyen, Scott D. Boyd, Brian L. Le, Renan Sper, Sandra C. A. Nielsen, Tippi C. MacKenzie, Silvia Pineda |
| المصدر: | J Immunol Journal of immunology (Baltimore, Md. : 1950), vol 207, iss 10 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | T cell, Lymphocyte, Immunology, Receptors, Antigen, T-Cell, chemical and pharmacologic phenomena, Reproductive health and childbirth, Biology, Low Birth Weight and Health of the Newborn, Obstetric Labor, Immune system, Obstetric Labor, Premature, Clinical Research, Preterm, Pregnancy, Receptors, Infant Mortality, medicine, Immunology and Allergy, Humans, Premature, B cell, Pediatric, Fetus, Repertoire, Infant, Newborn, Infant, Gestational age, Perinatal Period - Conditions Originating in Perinatal Period, T-Cell, Newborn, Complementarity Determining Regions, Good Health and Well Being, medicine.anatomical_structure, Antigen, Cord blood, embryonic structures, Premature Birth, Female, Clinical and Human Immunology, Immunoglobulin Heavy Chains, Infant, Premature |
| الوصف: | Preterm labor (PTL) is the leading cause of neonatal morbidity and mortality worldwide. Whereas many studies have investigated the maternal immune responses that cause PTL, fetal immune cell activation has recently been raised as an important contributor to the pathogenesis of PTL. In this study, we analyzed lymphocyte receptor repertoires in maternal and cord blood from 14 term and 10 preterm deliveries, hypothesizing that the high prevalence of infection in patients with PTL may result in specific changes in the T cell and B cell repertoires. We analyzed TCR β-chain (TCR-β) and IgH diversity, CDR3 lengths, clonal sharing, and preferential usage of variable and joining gene segments. Both TCR-β and IgH repertoires had shorter CDR3s compared with those in maternal blood. In cord blood samples, we found that CDR3 lengths correlated with gestational age, with shorter CDR3s in preterm neonates suggesting a less developed repertoire. Preterm cord blood displayed preferential usage of a number of genes. In preterm pregnancies, we observed significantly higher prevalence of convergent clones between mother/baby pairs than in term pregnancies. Together, our results suggest the repertoire of preterm infants displays a combination of immature features and convergence with maternal TCR-β clones compared with that of term infants. The higher clonal convergence in PTL could represent mother and fetus both responding to a shared stimulus like an infection. These data provide a detailed analysis of the maternal–fetal immune repertoire in term and preterm patients and contribute to a better understanding of neonate immune repertoire development and potential changes associated with PTL. |
| وصف الملف: | application/pdf |
| تدمد: | 1550-6606 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05a27874f6150b67b97e9e8388146c55Test https://pubmed.ncbi.nlm.nih.gov/34654689Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....05a27874f6150b67b97e9e8388146c55 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 |
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