Assessment of Rauvolfia nukuhivensis: from uses to biological ac-tivities and chemodiversity
| العنوان: | Assessment of Rauvolfia nukuhivensis: from uses to biological ac-tivities and chemodiversity |
|---|---|
| المؤلفون: | Gaël Lecellier, Luis M. Botana, Mael Nicolas, Marc Gaysinski, E. Alonso-Lopes, Sara F. Ferreiro, Soizic Prado, Olivier P. Thomas, Nicolas J. Martin, Phila Raharivelomanana, Florent Barbault, Christian Paetz |
| المساهمون: | Ecosystèmes Insulaires Océaniens (UMR 241) (EIO), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD), Department of Pharmacology, Universidade de Santiago de Compostela, Muséum national d'Histoire naturelle (MNHN), Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS (UMR_7086)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Géoazur (GEOAZUR 7329), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de la Côte d'Azur, Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Max Planck Institute for Chemical Ecology, Max-Planck-Gesellschaft, Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Observatoire de la Côte d'Azur, Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA), Université de la Polynésie Française (UPF)-Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Université Paris Diderot - Paris 7 (UPD7)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Côte d'Azur (UCA)-Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Observatoire de la Côte d'Azur, Université Côte d'Azur (UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA) |
| المصدر: | Planta Medica Planta Medica, Georg Thieme Verlag, 2016, 81 (S 01), pp.S1-S381. ⟨10.1055/s-0036-1596521⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Pharmacology, Rauvolfia, Phylogenetic tree, Indole alkaloid, Organic Chemistry, Pharmaceutical Science, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, 15. Life on land, Biology, biology.organism_classification, DNA barcoding, 3. Good health, Analytical Chemistry, Complementary and alternative medicine, Genus, visual_art, Drug Discovery, Botany, visual_art.visual_art_medium, Rauvolfia nukuhivensis, Molecular Medicine, Bioassay, Bark |
| الوصف: | International audience; Rauvolfia genus, biogeographically widespread in tropical areas, includes many species used in traditional medicine. Studies of these species showed that their uses are related to their bioactive content mostly composed by alkaloid compounds. Rauvolfia nukuhivensis, called locally “tueiao”, is an endemic species grown in Nuku-Hiva island located in Marquesas archi-pelago where the bark is used for intimate woman care [1]. Ethnopharmacological approach was adopted to assess this plant. For that purpose, firstly, we checked if the plant extract played an antiseptic role within antimicrobial effects by testing the extract and contents on bacterial and fungi strains (Escherichia coli, Staphylococcus aureus, Candida albicans and Aspergillus niger) and bioassay results showed moderate to low activities. Then, we supposed that traditional medicinal uses of this plant may be linked to biological activities having close relationship with secretory mechanisms regulation. This hypothesis was investigated by the ion channels inhibition related to osmotic exchanges and then, inhibiting effects on hNav1.6 currents and hERG channel were so investigated. Some alkaloidal constituents, and more especially nukuhivensiums, were shown to significantly induce a reduction of IKr amplitude (hERG current). According to these biological activities, a computational study through docking was performed in order to illustrate these results [2,3]. The chemodiversity of the alkaloids from the bark of this plant was assessed by three inte-grated approaches mainly: structural elucidation, phylogenetic analysis and a putative biosynthesis hypothesis statement. Structural elucidation by spectroscopic means (MS, NMR) led to the identification of 13 major constituents belonging to four distinct indole alkaloid skeletons (ajmalane, sarpagane, macroline and β-carboline) within six new naturally occuring compounds. As the Rauvolfia genus is well distributed in the Pacific region, we carried out a phylogenetic study (using DNA barcoding method) of Rauvolfia species, endemic or indigenous in Oceania, as a second approach, aiming to a better understanding of the occurence and distribution of these alkaloids. The very rare co-occurence of these alkaloids belonging to four different skeletons inspired to set up a putative biosynthesis of these components, as a third way of investigation of their chemodiversity |
| اللغة: | English |
| تدمد: | 0032-0943 1439-0221 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c4fb46bc61699fc4c0c24ee4f756039Test https://hal.archives-ouvertes.fr/hal-02148636Test |
| رقم الانضمام: | edsair.doi.dedup.....7c4fb46bc61699fc4c0c24ee4f756039 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00320943 14390221 |
|---|