DSP Toxin Distribution across Organs in Mice after Acute Oral Administration
| العنوان: | DSP Toxin Distribution across Organs in Mice after Acute Oral Administration |
|---|---|
| المؤلفون: | Toshiyuki Suzuki, Luis M. Botana, Mercedes R. Vieytes, Ryuichi Watanabe, M. Carmen Louzao, Paula Abal, Celia Costas, Natalia Vilariño, Ana M. Botana |
| المساهمون: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica, Universidade de Santiago de Compostela. Departamento de Fisioloxía, Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía |
| المصدر: | Marine Drugs Volume 19 Issue 1 Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) Marine Drugs, Vol 19, Iss 23, p 23 (2021) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Okadaic acid, LC/MS/MS, Pharmaceutical Science, Pharmacology, medicine.disease_cause, Mass Spectrometry, Article, 03 medical and health sciences, chemistry.chemical_compound, Mice, okadaic acid, Drug Discovery, medicine, Toxicokinetics, Animals, Shellfish Poisoning, Large intestine, Tissue Distribution, dinophysistoxin-2, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), lcsh:QH301-705.5, dinophysistoxin-1, Chromatography, High Pressure Liquid, 030304 developmental biology, Shellfish, 0303 health sciences, Gastrointestinal tract, Dose-Response Relationship, Drug, Toxin, Stomach, 030302 biochemistry & molecular biology, Toxicokinetic, Small intestine, toxicokinetic, 3. Good health, Intestines, Mice, Inbred C57BL, Dinophysistoxin-1, medicine.anatomical_structure, chemistry, lcsh:Biology (General), Toxicity, Dinophysistoxin-2, Female, Marine Toxins |
| الوصف: | Okadaic acid (OA) and its main structural analogs dinophysistoxin-1 (DTX1) and dinophysistoxin-2 (DTX2) are marine lipophilic phycotoxins distributed worldwide that can be accumulated by edible shellfish and can cause diarrheic shellfish poisoning (DSP). In order to study their toxicokinetics, mice were treated with different doses of OA, DTX1, or DTX2 and signs of toxicity were recorded up to 24 h. Toxin distribution in the main organs from the gastrointestinal tract was assessed by liquid chromatography-mass spectrometry (LC/MS/MS) analysis. Our results indicate a dose-dependency in gastrointestinal absorption of these toxins. Twenty-four hours post-administration, the highest concentration of toxin was detected in the stomach and, in descending order, in the large intestine, small intestine, and liver. There was also a different toxicokinetic pathway between OA, DTX1, and DTX2. When the same toxin doses are compared, more OA than DTX1 is detected in the small intestine. OA and DTX1 showed similar concentrations in the stomach, liver, and large intestine tissues, but the amount of DTX2 is much lower in all these organs, providing information on DSP toxicokinetics for human safety assessment The research leading to these results received funding from the following FEDER cofunded grants: Ministerio de Ciencia e Innovación AGL2016-78728-R (AEI/FEDER, UE), IISCIII/PI19/001248; Conselleria de Cultura, Educacion e Ordenación Universitaria, Xunta de Galicia, 2017 GRC GI-1682 (ED431C 2017/01); and European Union Interreg AlertoxNet EAPA-317-2016, Interreg Agritox EAPA-998-2018, and H2020 778069-EMERTOX. Celia Costas is supported by a fellowship from the Ministerio de Ciencia, Innovación y Universidades grant FPU18/05681 SI |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1660-3397 |
| DOI: | 10.3390/md19010023 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2bb0037bf3cdd1133c9cc8208420911Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e2bb0037bf3cdd1133c9cc8208420911 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16603397 |
|---|---|
| DOI: | 10.3390/md19010023 |