First molecular evidence of bovine hemoplasma species (Mycoplasma spp.) in water buffalo and dairy cattle herds in Cuba
| العنوان: | First molecular evidence of bovine hemoplasma species (Mycoplasma spp.) in water buffalo and dairy cattle herds in Cuba |
|---|---|
| المؤلفون: | Regina Hofmann-Lehmann, Evelyn Lobo Rivero, Adrian Alberto Díaz-Sánchez, Belkis Corona-González, Ernesto Vega Cañizares, Osvaldo Fonseca Rodríguez, Marina L. Meli, Dasiel Obregón Alvarez |
| المساهمون: | University of Zurich, Hofmann-Lehmann, Regina |
| المصدر: | Parasites & Vectors, Vol 12, Iss 1, Pp 1-9 (2019) Parasites & Vectors |
| بيانات النشر: | Umeå universitet, Institutionen för epidemiologi och global hälsa, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Veterinary medicine, 2405 Parasitology, medicine.disease_cause, Mycoplasma, 0302 clinical medicine, RNA, Ribosomal, 16S, Prevalence, 11434 Center for Clinical Studies, Coinfection, Cuba, Hemotropic mycoplasma, Dairying, time PCR, Infectious Diseases, 11404 Department of Clinical Diagnostics and Services, Female, Livestock, Real, Buffaloes, Anemia, 030231 tropical medicine, Cattle Diseases, 610 Medicine & health, |
| الوصف: | Background Hemotropic mycoplasmas (aka hemoplasmas) are small bacteria which cause infectious anemia in several mammalian species including humans. Information on hemoplasma infections in Cuban bovines remains scarce and no studies applying molecular methods have been performed so far. The aim of the present study was to utilize real-time PCR and sequence analysis to investigate dairy cattle and buffalo from Cuba for the presence of bovine hemoplasma species. Results A total of 80 blood samples from 39 buffalo and 41 dairy cattle were investigated for the presence of Mycoplasma wenyonii and “Candidatus Mycoplasma haemobos” using two species-specific real-time TaqMan PCR assays. PCR results revealed overall 53 (66.2%; 95% CI: 55.3–75.7%) positive animals for M. wenyonii and 33 (41.2%; 95% CI: 31.1–52.2%) for “Ca. M. haemobos”; the latter were all co-infections with M. wenyonii. The sample prevalences were similar in cattle and buffalo. Based on the sequence analysis of the nearly full-length 16S rRNA gene from two cattle and two buffalo, the presence of M. wenyonii and “Ca. M. haemobos” was confirmed. Statistical analysis revealed that buffalo and cattle one year of age or older were more frequently infected with M. wenyonii or “Ca. M. haemobos” than younger animals. PCR-positivity was not associated with anemia; however, the infection stage was unknown (acute infection versus chronic carriers). Conclusions The high occurrence of bovine hemoplasma infections in buffalo and dairy cattle may have a significant impact on Cuban livestock production. To the best of our knowledge, this is the first molecular evidence of bovine hemoplasma species infection in dairy cattle and buffalo from Cuba and the Caribbean. |
| وصف الملف: | application/pdf; s13071-019-3325-y - application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac70611bac86b9203aa89fbbf5732cd3Test http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-156876Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ac70611bac86b9203aa89fbbf5732cd3 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |