Matrix-assisted laser desorption/ionization time of flight mass spectrometry identification of Vibrio (Listonella) anguillarum isolated from sea bass and sea bream
| العنوان: | Matrix-assisted laser desorption/ionization time of flight mass spectrometry identification of Vibrio (Listonella) anguillarum isolated from sea bass and sea bream |
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| المؤلفون: | Marialetizia Fioravanti, Rozelindra Čož-Rakovac, Ivančica Strunjak-Perović, Snježana Kazazić, Krunoslav Bojanić, Sanja Babić, Daniela Florio, Natalija Topić Popović |
| المساهمون: | Kazazic S.P., Popovic N.T., Strunjak-Perovic I., Babic S., Florio D., Fioravanti M., Bojanic K., Coz-Rakovac R. |
| المصدر: | PLoS ONE PLOS ONE PLoS ONE, Vol 14, Iss 11, p e0225343 (2019) |
| بيانات النشر: | Public Library of Science, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Molecular biology, DNA hybridization, Marine and Aquatic Sciences, Pathology and Laboratory Medicine, Listonella anguillarum, Mass Spectrometry, Analytical Chemistry, chemistry.chemical_compound, Spectrum Analysis Techniques, Peptide mass fingerprinting, Medicine and Health Sciences, Agar, Cluster Analysis, Marine Fish, Food science, Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, Incubation, Listonella, Multidisciplinary, biology, Chemistry, MALDI-TOF MS, Vibrio anguillarum, Eukaryota, Mass Spectra, sea ba, Marine Bacteria, Bacterial Pathogens, Medical Microbiology, Physical Sciences, Vertebrates, Medicine, Pathogens, Research Article, Veterinary Medicine, food.ingredient, Science, 030106 microbiology, Marine Biology, Research and Analysis Methods, Microbiology, 03 medical and health sciences, food, Signs and Symptoms, Diagnostic Medicine, Animals, 14. Life underwater, Sea bass, Microbial Pathogens, Vibrio, Vibrio (Listonella) anguillarum, Molecular probe techniques, Bacteria, Organisms, Biology and Life Sciences, Septicemia, biology.organism_classification, Probe hybridization, 030104 developmental biology, Fish, Molecular biology techniques, Tryptone, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Earth Sciences, Bass, sea bream |
| الوصف: | Vibrio(Listonella)anguillarumis a pathogenic bacterium causing septicaemia in a wide range of marine organisms and inducing severe mortalities, thus it is crucial to conduct its accurate and rapid identification. The aim of this study was to assess MALDI-TOF MS as a method of choice for identification of clinicalV.anguillarumisolates from affected marine fish. Since the method accuracy might be influenced by the type of the medium used, as well as by the incubation conditions, we testedV.anguillarumisolates grown on standard media with and without the addition of NaCl, cultured at three incubation temperatures, and at three incubation periods. The best scores were retrieved forV.anguillarumstrains grown on NaCl-supplemented tryptone soy agar (TSA) at 22°C and incubated for 48h (100% identification to species level; overall score 2.232), followed by incubation at 37°C and 48h (100% to species level; score 2.192). The strains grown on non-supplemented TSA gave the best readings when incubated at 22°C for 72h (100% identification to species level; overall score 2.182), followed by incubation at 15°C for 72h (100% to species level; score 2.160). Unreliable identifications and no-identifications were growing with the incubation duration at 37°C, on both media, amounting to 88.89% for 7d incubation on supplemented TSA, and 92.60% for 7d incubation on non-supplemented TSA. The age of the cultured strains and use of media significantly impacted the mass spectra, demonstrating that for reliable identification, MALDI-TOF MS protein fingerprinting with the on-target extraction should be performed on strains grown on a NaCl-supplemented medium at temperatures between 15 and 22°C, incubated for 48–72 hours. |
| وصف الملف: | application/pdf; STAMPA |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19756067820f2d523592df5002a66ca7Test http://fulir.irb.hr/5224Test/ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....19756067820f2d523592df5002a66ca7 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |