Crambescin C1 Exerts a Cytoprotective Effect on HepG2 Cells through Metallothionein Induction
| العنوان: | Crambescin C1 Exerts a Cytoprotective Effect on HepG2 Cells through Metallothionein Induction |
|---|---|
| المؤلفون: | Luis M. Botana, Olivier P. Thomas, Juan A. Rubiolo, María Roel, Mercedes R. Vieytes, Eva Ternon |
| المساهمون: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Universidade de Santiago de Compostela. Departamento de Fisioloxía, Universidade de Santiago de Compostela [Spain] (USC ), Institut de Chimie de Nice (ICN), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Department Farmacologia, University of Santiago de Compostela, FEDER cofunded-grants. CDTI and Technological Funds, supported by Ministerio de Economía y Competitividad, AGL2012-40185-CO2-01 and Consellería de Cultura, Educación e Ordenación Universitaria, GRC2013-016, and by Axencia Galega de Innovación, Spain, ITC-20133020 SINTOX, IN852A 2013/16-3 MYTIGAL. CDTIunderISIP Programme, Spain, IDI-20130304 APTAFOOD. European Union’s 7th Framework Programme managed by Research Executive Agency (FP7/2007-2013) under grant agreement Nos. 265409 µAQUA, 315285 CIGUATOOLS and 312184 PHARMASEA., Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA) |
| المصدر: | Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) Marine Drugs, Vol 13, Iss 8, Pp 4633-4653 (2015) Marine drugs Marine drugs, MDPI, 2015, 13 (8), pp.4633--4653. ⟨10.3390/md13084633⟩ Marine drugs, 2015, 13 (8), pp.4633--4653. ⟨10.3390/md13084633⟩ Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Marine Drugs Volume 13 Issue 8 Pages 4633-4653 |
| بيانات النشر: | MDPI, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Pharmaceutical Science, antioxidant effect, Crambescin-C1, Transcriptome, Biological Factors, Crambe Sponge, chemistry.chemical_compound, Crambe crambe, Sponge-derived compounds, Drug Discovery, Metallothionein, Guanidine, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), lcsh:QH301-705.5, Hep G2 Cells, Antioxidant effect, Cytoprotection, 3. Good health, Cell biology, Hepg2 cells, Biology, Resting Phase, Cell Cycle, crambescin-C1, Article, Alkaloids, Cell Line, Tumor, Botany, Animals, Humans, cell cycle inhibition, Cell cycle inhibition, Cell Proliferation, Transcriptome profiling, Cell growth, G1 Phase, transcriptome profiling, metallothionein, Crambescin-A1, Pyrimidines, chemistry, crambescin-A1, lcsh:Biology (General), [SDE.BE]Environmental Sciences/Biodiversity and Ecology, sponge-derived compounds |
| الوصف: | The Mediterranean marine sponge Crambe crambe is the source of two families of guanidine alkaloids known as crambescins and crambescidins. Some of the biological effects of crambescidins have been previously reported while crambescins have undergone little study. Taking this into account, we performed comparative transcriptome analysis to examine the effect of crambescin-C1 (CC1) on human tumor hepatocarcinoma cells HepG2 followed by validation experiments to confirm its predicted biological activities. We report herein that, while crambescin-A1 has a minor effect on these cells, CC1 protects them against oxidative injury by means of metallothionein induction even at low concentrations. Additionally, at high doses, CC1 arrests the HepG2 cell cycle in G0/G1 and thus inhibits tumor cell proliferation. The findings presented here provide the first detailed approach regarding the different effects of crambescins on tumor cells and provide a basis for future studies on other possible cellular mechanisms related to these bioactivities FEDER cofunded-grants. CDTI and Technological Funds, supported by Ministerio de Economía y Competitividad, AGL2012-40185-CO2-01 and Consellería de Cultura, Educación e Ordenación Universitaria, GRC2013-016, and by Axencia Galega de Innovación, Spain, ITC-20133020 SINTOX, IN852A 2013/16-3 MYTIGAL. CDTIunderISIP Programme, Spain, IDI-20130304 APTAFOOD. European Union’s 7th Framework Programme managed by Research Executive Agency (FP7/2007-2013) under grant agreement Nos. 265409 µAQUA, 315285 CIGUATOOLS and 312184 PHARMASEA SI |
| وصف الملف: | application/pdf |
| تدمد: | 1660-3397 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c27f12c14abe1b1e1e7928ed60a72fecTest http://hdl.handle.net/10347/23611Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c27f12c14abe1b1e1e7928ed60a72fec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16603397 |
|---|