Oral Toxicity of Okadaic Acid in Mice: Study of Lethality, Organ Damage, Distribution and Effects on Detoxifying Gene Expression
| العنوان: | Oral Toxicity of Okadaic Acid in Mice: Study of Lethality, Organ Damage, Distribution and Effects on Detoxifying Gene Expression |
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| المؤلفون: | José Manuel Cifuentes, H. López-Alonso, Andrés C. Vieira, Luis M. Botana, Juan A. Rubiolo, Mercedes R. Vieytes, Paz Otero, Amparo Alfonso, F. V. Vega, Roberto Bermúdez |
| المساهمون: | Universidade de Santiago de Compostela. Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias, Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica, Universidade de Santiago de Compostela. Departamento de Fisioloxía |
| المصدر: | Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) Toxins; Volume 5; Issue 11; Pages: 2093-2108 Toxins Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Toxins, Vol 5, Iss 11, Pp 2093-2108 (2013) |
| بيانات النشر: | MDPI, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Okadaic acid, Antioxidant, Health, Toxicology and Mutagenesis, medicine.medical_treatment, lcsh:Medicine, Administration, Oral, Gene Expression, Kidney, Toxicology, medicine.disease_cause, Antioxidants, Feces, chemistry.chemical_compound, Quantitative PCR, Mice, superoxide dismutase 1, Intestine, Small, 0303 health sciences, Stomach, catalase, 030302 biochemistry & molecular biology, Catalase, Immunohistochemistry, 3. Good health, medicine.anatomical_structure, Liver, Inactivation, Metabolic, Toxicity, histopathology, Female, Diarrhea, mice, Histopathology, Biology, liver, Article, Superoxide dismutase, 03 medical and health sciences, In vivo, okadaic acid, medicine, Animals, 030304 developmental biology, Superoxide dismutase 1, lcsh:R, diarrhea, immunohistochemistry, quantitative PCR, Molecular biology, Small intestine, Oxidative Stress, chemistry, biology.protein, Oxidative stress |
| الوصف: | In vivo, after administration by gavage to mice and rats, okadaic acid has been reported to produce lesions in liver, small intestine and forestomach. Because several reports differ in the damage detected in different organs, and on okadaic acid distribution after consumption, we determined the toxicity of this compound after oral administration to mice. After 24 hours, histopathological examination showed necrotic foci and lipid vacuoles in the livers of intoxicated animals. By immunohistochemical analysis, we detected this toxin in the liver and kidneys of intoxicated animals. Okadaic acid induces oxidative stress and can be activated in vitro into reactive compounds by the post-mitochondrial S9 fraction, so we studied the okadaic effect on the gene expression of antioxidant and phase II detoxifying enzymes in liver. We observed a downregulation in the expression of these enzymes and a reduction of protein expression of catalase and superoxide dismutase 1 in intoxicated animals The research leading to these results has received funding from the following FEDER cofunded-grants: From Ministerio de Ciencia y Tecnología, Spain: AGL2009-13581-CO2-01, AGL2012-40485-CO2-01. From Xunta de Galicia, Spain: 10PXIB261254 PR. From the European Union’s Seventh Framework Programme managed by REA–Research Executive Agency (FP7/2007-2013) under grant agreement Nos. 265896 BAMMBO, 265409 µAQUA, and 262649 BEADS, 315285 Ciguatools and 312184 PharmaSea. From the Atlantic Area Programme (Interreg IVB Trans-national): 2009-1/117 Pharmatlantic SI |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d19ba0360e6be15293d37a0713a8625eTest http://hdl.handle.net/10347/23597Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d19ba0360e6be15293d37a0713a8625e |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |