Analysis of the recombination landscape of hexaploid bread wheat reveals genes controlling recombination and gene conversion frequency
| العنوان: | Analysis of the recombination landscape of hexaploid bread wheat reveals genes controlling recombination and gene conversion frequency |
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| المؤلفون: | Laura-Jayne Gardiner, Ryan Joynson, Thomas Brabbs, Bernardo J. Clavijo, Neil Hall, Anthony Hall, Jonathan M. Wright, Luzie U. Wingen, James D. Higgins, Simon Griffiths, Paul Bailey |
| المصدر: | Genome Biology, Vol 20, Iss 1, Pp 1-16 (2019) Genome Biology |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, lcsh:QH426-470, QTL, RecQ helicase, Crossover, Quantitative trait locus, Biology, 01 natural sciences, Genome, Chromosomal crossover, Polyploidy, 03 medical and health sciences, 0302 clinical medicine, Crossing Over, Genetic, Gene conversion, Gene, lcsh:QH301-705.5, Triticum, 030304 developmental biology, 2. Zero hunger, Genetics, 0303 health sciences, Genetic diversity, Whole Genome Sequencing, Research, Haplotype, food and beverages, Recombination, lcsh:Genetics, lcsh:Biology (General), Wheat, Genome, Plant, 030217 neurology & neurosurgery, 010606 plant biology & botany |
| الوصف: | Background Sequence exchange between homologous chromosomes through crossing over and gene conversion is highly conserved among eukaryotes, contributing to genome stability and genetic diversity. A lack of recombination limits breeding efforts in crops; therefore, increasing recombination rates can reduce linkage drag and generate new genetic combinations. Results We use computational analysis of 13 recombinant inbred mapping populations to assess crossover and gene conversion frequency in the hexaploid genome of wheat (Triticum aestivum). We observe that high-frequency crossover sites are shared between populations and that closely related parents lead to populations with more similar crossover patterns. We demonstrate that gene conversion is more prevalent and covers more of the genome in wheat than in other plants, making it a critical process in the generation of new haplotypes, particularly in centromeric regions where crossovers are rare. We identify quantitative trait loci for altered gene conversion and crossover frequency and confirm functionality for a novel RecQ helicase gene that belongs to an ancient clade that is missing in some plant lineages including Arabidopsis. Conclusions This is the first gene to be demonstrated to be involved in gene conversion in wheat. Harnessing the RecQ helicase has the potential to break linkage drag utilizing widespread gene conversions. Electronic supplementary material The online version of this article (10.1186/s13059-019-1675-6) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c10aa4b1eb5683a38bcd4cdccbcb73aTest http://link.springer.com/article/10.1186/s13059-019-1675-6Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3c10aa4b1eb5683a38bcd4cdccbcb73a |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |