FGF9 inhibits browning program of white adipocytes and associates with human obesity
| العنوان: | FGF9 inhibits browning program of white adipocytes and associates with human obesity |
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| المؤلفون: | Weiqing Wang, Lingyun Tang, Zhugang Wang, Jie Hong, Guang Ning, Yingkai Sun, Rui Wang, Ruixin Liu, Jiqiu Wang, Wen Liu, Wen Li, Shaoqian Zhao |
| المصدر: | Journal of Molecular Endocrinology. 62:79-90 |
| بيانات النشر: | Bioscientifica, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Fibroblast Growth Factor 9, Male, 0301 basic medicine, medicine.medical_specialty, FGF21, Adolescent, Adipose Tissue, White, Adipocytes, White, Adipose tissue, White adipose tissue, Biology, Fibroblast growth factor, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Adipose Tissue, Brown, Internal medicine, Adipocyte, medicine, Animals, Humans, Obesity, RNA, Messenger, Molecular Biology, Gene knockdown, Cell Differentiation, Thermogenesis, Stromal vascular fraction, Hypoxia-Inducible Factor 1, alpha Subunit, Mice, Inbred C57BL, stomatognathic diseases, 030104 developmental biology, Gene Expression Regulation, chemistry, Female, 030217 neurology & neurosurgery, Signal Transduction |
| الوصف: | Browning of white adipose tissue has been proven to be a potential target to fight against obesity and its metabolic commodities, making the exploration of molecules involved in browning process important. Among those browning agents reported recently, FGF21 play as a quite promising candidate for treating obesity for its obvious enhancement of thermogenic capacity in adipocyte and significant improvement of metabolic disorders in both mice and human. However, whether other members of fibroblast growth factor (FGF) family play roles in adipose thermogenesis and obese development is still an open question. Here, we examined the mRNA expression of all FGF family members in three adipose tissues of male C57BL/6 mice and found that FGF9 is highly expressed in adipose tissue and decreased under cold stress. Furthermore, FGF9 treatment inhibited thermogenic genes in the process of beige adipocytes differentiation from stromal vascular fraction (SVF) in a dose-dependent manner. Similar results were obtained with FGF9 overexpression. Consistently, knockdown of FGF9 in SVF cells by using lentiviral shRNA increased thermogenic genes in differentiated beige adipocytes. RNA sequencing analysis revealed a significant increment of hypoxia-inducible factor (HIF) pathway in the early stage of beige adipocytes differentiation under FGF9 treatment, which was validated by real-time PCR. FGF9 expression was increased in subcutaneous WAT of obese human and mice. This study shows that adipose-derived FGF9 play as an inhibitory role in the browning of white adipocytes. Activation of hypoxia signaling at early stage of adipose browning process may contribute to this anti-thermogenic effect of FGF9. |
| تدمد: | 1479-6813 0952-5041 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3885669eb3441f7a2c94123353756f5aTest https://doi.org/10.1530/jme-18-0151Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3885669eb3441f7a2c94123353756f5a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14796813 09525041 |
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