Differences between Platelets Derived from Neonatal Cord Blood and Adult Peripheral Blood Assessed by Mass Spectrometry
| العنوان: | Differences between Platelets Derived from Neonatal Cord Blood and Adult Peripheral Blood Assessed by Mass Spectrometry |
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| المؤلفون: | Stokhuijzen, Eva, Koornneef, Johanna M, Nota, Benjamin, van den Eshof, Bart Laurens, van Alphen, Floris Pieter Joachim, van den Biggelaar, Maartje, Van Der Zwaan, Carmen, Kuijk, Carlijn, Mertens, Koen, Fijnvandraat, Karin, Meijer, Alexander Benjamin, Afd Pharmaceutics, Pharmaceutics, Biomolecular Mass Spectrometry and Proteomics |
| المساهمون: | Graduate School, ARD - Amsterdam Reproduction and Development, ACS - Amsterdam Cardiovascular Sciences, Paediatric Infectious Diseases / Rheumatology / Immunology, ACS - Pulmonary hypertension & thrombosis, Afd Pharmaceutics, Pharmaceutics, Biomolecular Mass Spectrometry and Proteomics |
| المصدر: | Journal of Proteome Research, 16(10), 3567. American Chemical Society Journal of proteome research, 16(10), 3567-3575. American Chemical Society |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Adult, Blood Platelets, Male, Proteomics, 0301 basic medicine, Platelet Aggregation, proteome, Integrin, 030204 cardiovascular system & hematology, Biochemistry, Umbilical cord, Flow cytometry, 03 medical and health sciences, Thromboxane A2, chemistry.chemical_compound, 0302 clinical medicine, medicine, Humans, Receptor, PAR-1, Platelet, Receptor, mass spectrometry, chemistry.chemical_classification, medicine.diagnostic_test, biology, Chemistry, Infant, Newborn, General Chemistry, Middle Aged, Fetal Blood, Platelet Activation, Molecular biology, 030104 developmental biology, medicine.anatomical_structure, Cord blood, platelets, cord blood, biology.protein, Female, Receptors, Thrombin, Collagen, Transcriptome, Glycoprotein |
| الوصف: | It has been proposed that differences may exist between umbilical cord blood (CB) platelets and adult peripheral blood (APB) platelets, including altered protein levels of the main platelet integrins. We have now compared the protein expression profiles of CB and APB platelets employing a label-free comparative proteomics approach. Aggregation studies showed that CB platelets effectively aggregate in the presence of thromboxane A2 analogue, collagen, and peptide agonists of the proteinase-activated receptors 1 and 4. In agreement with previous studies, higher concentrations of the agonists were required to initiate aggregation in the CB platelets. Mass spectrometry analysis revealed no significant difference in the expression levels of critical platelet receptors like glycoprotein (GP)Ib, GPV, GPIX, and integrin αIIbβ3. This was confirmed using flow cytometry-based approaches. Gene ontology enrichment analysis revealed that elevated proteins in CB platelets were in particular enriched in proteins contributing to mitochondrial energy metabolism processes. The reduced proteins were enriched in proteins involved in, among others, platelet degranulation and activation. In conclusion, this study reveals that the CB and APB platelets are distinct. In particular, changes were observed for proteins that belong to metabolic and energy generation processes and not for the critical adhesive platelet integrins and glycoproteins. |
| وصف الملف: | application/pdf |
| تدمد: | 1535-3907 1535-3893 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd227a444bcb1091acfb28800e45f753Test https://doi.org/10.1021/acs.jproteome.7b00298Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....cd227a444bcb1091acfb28800e45f753 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
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