Impact of Antibiotic-Induced Depletion of Gut Microbiota and Selenium Supplementation on Plasma Selenoproteome and Metal Homeostasis in a Mice Model
| العنوان: | Impact of Antibiotic-Induced Depletion of Gut Microbiota and Selenium Supplementation on Plasma Selenoproteome and Metal Homeostasis in a Mice Model |
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| المؤلفون: | Maria Carmen Collado, Belén Callejón-Leblic, Nieves Abril, Marta Selma-Royo, Tamara García-Barrera |
| المساهمون: | Ministerio de Ciencia, Innovación y Universidades (España), Junta de Andalucía, European Commission, Fundación Ramón Areces |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) |
| بيانات النشر: | American Chemical Society, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, Antioxidant, Heteroatom-tagged proteomics, medicine.medical_treatment, chemistry.chemical_element, Gut flora, digestive system, 01 natural sciences, Microbiology, Mice, Selenium, Selenio, RNA, Ribosomal, 16S, Lactobacillus, medicine, 23 Química, Animals, Homeostasis, ICP-MS, Selenoproteins, chemistry.chemical_classification, Mice, Inbred BALB C, Suplementacion, biology, Glutathione peroxidase, Microbiota, 010401 analytical chemistry, General Chemistry, Selenoproteinas, biology.organism_classification, Anti-Bacterial Agents, Gastrointestinal Microbiome, 0104 chemical sciences, stomatognathic diseases, chemistry, Dietary Supplements, Chemical speciation, General Agricultural and Biological Sciences, 2301 Química Analítica, Bacteria, 010606 plant biology & botany, Ruminococcaceae |
| الوصف: | Determinación de metales y Selenoproteinas y su interacción con la Microbiota intestinal tras la ingesta de suplementos de selenio y la administración de antibióticos a ratones Mus musculus. Selenium (Se) is a micronutrient involved in important health functions and it has been suggested to shape gut microbiota. Limited information on Se assimilation by gut microbes and the possible link with selenoproteins are available. For this purpose, conventional and gut microbiota-depleted BALB/c mice were fed a Se-supplemented diet. The absolute quantification of mice plasma selenoproteins was performed for the first time using heteroatom-tagged proteomics. The gut microbiota profile was analyzed by 16S rRNA gene sequencing. Se-supplementation modulated the concentration of the antioxidant glutathione peroxidase and the Se-transporter selenoalbumin as well as the metal homeostasis, being influenced by microbiota disruption, which suggests an intertwined mechanism. Se also modulated microbiota diversity and richness and increased the relative abundance of some health- relevant taxa (e.g., families Christensenellaceae, Ruminococcaceae, and Lactobacillus genus). This study demonstrated the potential beneficial effects of Se on gut microbiota, especially after antibiotic-treatment and the first associations between specific bacteria and plasma selenoproteins. This work was supported by the projects PG2018-096608-BC21 from the Spanish Ministry of Science and innovation (MINECO) and UHU-1256905 from the FEDER Andalusian Operative Program 2014−2020 (Ministry of Economy, Knowledge, Business and Universities, Regional Government of Andalusia, Spain). The authors would like to acknowledge thesupportfromTheRamonArecesFoundation(ref. CIVP19A5918). The authors are grateful to FEDER (European Community) for financial support, grant UNHU13-1E1611. Funding for open access charge: Universidad de Huelva / CBUA |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d9c70ca353f755f8f2aa7e1fb8611b6Test http://hdl.handle.net/10261/247631Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7d9c70ca353f755f8f2aa7e1fb8611b6 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |