The mechanism for hexachlorocyclohexane-induced cytotoxicity and changes in intracellular Ca2+ homeostasis in cultured cerebellar granule neurons is different for the γ- and δ-isomers
| العنوان: | The mechanism for hexachlorocyclohexane-induced cytotoxicity and changes in intracellular Ca2+ homeostasis in cultured cerebellar granule neurons is different for the γ- and δ-isomers |
|---|---|
| المؤلفون: | Aase Frandsen, R Rosa, Eduard Rodríguez-Farré, Anna Pomés, Cristina Suñol, Arne Schousboe, Coral Sanfeliu |
| المساهمون: | Comisión Interministerial de Ciencia y Tecnología, CICYT (España), Generalitat de Catalunya, Ministerio de Sanidad (España), Danish Research Council, Lundbeck Foundation |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Academic Press, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Intracellular Fluid, Convulsants, Toxicology, Second Messenger Systems, Dantrolene, Calcium Chloride, Cerebellar Cortex, Structure-Activity Relationship, chemistry.chemical_compound, Isomerism, Lactate dehydrogenase, medicine, Animals, Homeostasis, Propidium iodide, Rats, Wistar, Cytotoxicity, Neurons, Pharmacology, Central Nervous System Depressants, Calcium Channel Blockers, Rats, Microscopy, Fluorescence, Verapamil, chemistry, Mechanism of action, Biochemistry, Biophysics, Calcium, Trypan blue, medicine.symptom, Hexachlorocyclohexane, Intracellular, medicine.drug |
| الوصف: | The cytotoxic action of the δ- and γ-isomers of hexachlorocyclohexane (HCH) as well as their ability to induce changes in intracellular Ca2+homeostasis was studied in cultured rat cerebellar granule neurons. Changes in the free intracellular Ca2+concentration ([Ca2+]i) related to Ca2+influx and release from intracellular stores were investigated using, in addition to the physiological incubation medium, media without added Ca2+(nominally Ca2+free) or containing verapamil or dantrolene, drugs which block influx through voltage-gated Ca2+channels and release from intracellular stores, respectively. Cytotoxicity was monitored using leakage of lactate dehydrogenase (LDH) and staining of damaged cells with either trypan blue or propidium iodide. In the latter case, when fluorescence microscopy was employed, undamaged cells were visualized using the vital stain calcein acetoxymethyl ester (calcein-AM). The δ-isomer of HCH was found to be more potent and active as a cytotoxic agent than the γ-isomer (lindane) and it was demonstrated that the moderate cytotoxic action of lindane could be ameliorated by dantrolene which on the other hand had no effect on cytotoxicity induced by δ-HCH. It was noticed that pronounced (50% increase in fluorescence) staining with propidium iodide was associated with only a marginal (20%) LDH leakage and staining of only a few ( This work has been supported financially by grants from the Spanish Cimissión Interministerial de Ciencia y Tecnologia (CICYT/SAF94-0076), the CIRIT, Generalitat de Catalunya (SGR 95-00551 and SGR 95-00445), in Fondo de Investigationes Sanitarias (FIS-95/1955), the CEC BIOTEC Program (BIOT-CT93-0224), as well as the Danish State Biotechnology Program (1991-95) and the Lundbeck Foundation. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d20b66d166e56df28a260c9dfa276d91Test http://hdl.handle.net/10261/270856Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d20b66d166e56df28a260c9dfa276d91 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |