Differential Pro-Inflammatory Responses of Astrocytes and Microglia Involve STAT3 Activation in Response to 1800 MHz Radiofrequency Fields
| العنوان: | Differential Pro-Inflammatory Responses of Astrocytes and Microglia Involve STAT3 Activation in Response to 1800 MHz Radiofrequency Fields |
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| المؤلفون: | Yonghui Lu, Zhengping Yu, Mindi He, Chunhai Chen, Lei Zhang, Chuan Liu, Yang Zhang, Huifeng Pi, Yue He, Shangcheng Xu, Zhou Zhou |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 9, p e108318 (2014) |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Macroglial Cells, Cell signaling, Radio Waves, lcsh:Medicine, Nitric Oxide Synthase Type II, Signal transduction, Mice, chemistry.chemical_compound, Animal Cells, Medicine and Health Sciences, Phosphorylation, lcsh:Science, Immune Response, CD11b Antigen, Multidisciplinary, Glial fibrillary acidic protein, biology, Microglia, Cell biology, Nitric oxide synthase, STAT signaling, medicine.anatomical_structure, Integrin alpha M, Cytokines, Cellular Types, medicine.symptom, Protein Binding, Research Article, STAT3 Transcription Factor, Immunology, Central nervous system, Glial Cells, Inflammation, Nitric Oxide, Dinoprostone, Cell Line, Nitric oxide, Electromagnetic Fields, Glial Fibrillary Acidic Protein, medicine, Animals, Microglial Cells, Neuroinflammation, Biology and life sciences, Gene Expression Profiling, lcsh:R, DNA, Gene Expression Regulation, nervous system, chemistry, Cyclooxygenase 2, Astrocytes, biology.protein, lcsh:Q |
| الوصف: | Microglia and astrocytes play important role in maintaining the homeostasis of central nervous system (CNS). Several CNS impacts have been postulated to be associated with radiofrequency (RF) electromagnetic fields exposure. Given the important role of inflammation in neural physiopathologic processes, we investigated the pro-inflammatory responses of microglia and astrocytes and the involved mechanism in response to RF fields. Microglial N9 and astroglial C8-D1A cells were exposed to 1800 MHz RF for different time with or without pretreatment with STAT3 inhibitor. Microglia and astrocytes were activated by RF exposure indicated by up-regulated CD11b and glial fibrillary acidic protein (GFAP). However, RF exposure induced differential pro-inflammatory responses in astrocytes and microglia, characterized by different expression and release profiles of IL-1β, TNF-α, IL-6, PGE2, nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2). Moreover, the RF exposure activated STAT3 in microglia but not in astrocytes. Furthermore, the STAT3 inhibitor Stattic ameliorated the RF-induced release of pro-inflammatory cytokines in microglia but not in astrocytes. Our results demonstrated that RF exposure differentially induced pro-inflammatory responses in microglia and astrocytes, which involved differential activation of STAT3 in microglia and astrocytes. Our data provide novel insights into the potential mechanisms of the reported CNS impacts associated with mobile phone use and present STAT3 as a promising target to protect humans against increasing RF exposure. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0675d8977ecdf0e710d8d522a0fd0a38Test https://doi.org/10.1371/journal.pone.0108318Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0675d8977ecdf0e710d8d522a0fd0a38 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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