نتائج البحث - "Hong Zhang"

1

Differentiation between thyroid‐associated orbitopathy and Graves’ disease by iTRAQ‐based quantitative proteomic analysis

المؤلفون: Hong Zhang, Yunqin Li, Zhijian Zhao, Jianshu Kang

المصدر: FEBS Open Bio, Vol 11, Iss 7, Pp 1930-1940 (2021)
FEBS Open Bio

الوصف: Graves’ ophthalmopathy, also known as thyroid‐associated orbitopathy (TAO), is the most common inflammatory eye disease in adults. The most common etiology for TAO is Graves’ disease (GD); however, proteomic research focusing on differences between GD and TAO is limited. This study aimed to identify differentially expressed proteins between thyroid‐associated orbitopathy (TAO) and GD. Furthermore, we sought to explore the pathogenesis of TAO and elucidate the differentiation process via specific markers. Serum samples of three patients with TAO, GD, and healthy controls, respectively, were collected. These samples were measured using the iTRAQ technique coupled with mass spectrometry. Differentially expressed proteins in TAO and GD were identified by proteomics; 3172 quantified proteins were identified. Compared with TAO, we identified 110 differential proteins (27 proteins were upregulated and 83 were downregulated). In addition, these differentially expressed proteins were closely associated with cellular processes, metabolic processes, macromolecular complexes, signal transduction, and the immune system. The corresponding functions were protein, calcium ion, and nucleic acid binding. Among the differential proteins, MYH11, P4HB, and C4A were markedly upregulated in TAO patients and have been reported to participate in apoptosis, autophagy, the inflammatory response, and the immune system. A protein–protein interaction network analysis was performed. Proteomics demonstrated valuable large‐scale protein‐related information for expounding the pathogenic mechanism underlying TAO. This research provides new insights and potential targets for studying GD with TAO.
Serum samples were collected from patients with TAO and Graves’ disease (GD), and healthy controls (three cases in each group). After extraction and quantification, the obtained proteins were treated with ITRAQ reagent and then analyzed using LC‐MS/MS. Finally, the identified differentially expressed proteins in TAO and GD were subjected to bioinformatics analysis, including GO analysis, KEGG analysis, and PPI analysis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fac36fa29ee2590da91b42373ae5b5cbTest
https://doaj.org/article/899fedea9f5e4680849a74e2e2da105aTest

2

Predictive factors of viral load high-risk events for virological failure in HIV/AIDS patients receiving long-term antiviral therapy

المؤلفون: Chuanyi Ning, Zhihao Meng, Shanfang Qin, Qing Lv, Hao Liang, Di Wei, Jingzhen Lai, Ke Lan, Hong Zhang, Lihua Huang, Pan Xue

المصدر: BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-11 (2021)
BMC Infectious Diseases

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, China, medicine.medical_specialty, 030106 microbiology, Viremia, Infectious and parasitic diseases, RC109-216, Logistic regression, 03 medical and health sciences, Virological failure, 0302 clinical medicine, Medical microbiology, Acquired immunodeficiency syndrome (AIDS), Internal medicine, medicine, Humans, Viral load, Treatment Failure, 030212 general & internal medicine, Proportional Hazards Models, Retrospective Studies, Acquired Immunodeficiency Syndrome, Proportional hazards model, business.industry, Incidence, HIV, Middle Aged, medicine.disease, Survival Rate, AIDS, Infectious Diseases, Anti-Retroviral Agents, Tropical medicine, HIV-1, Female, business, ART, Research Article

الوصف: Background In the era of anti-retroviral therapy (ART), the plasma HIV viral load (VL) is an important primary indicator for monitoring the HIV treatment response. To optimize the clinical management of HIV/AIDS patients, we investigated VL high-risk events related to virological failure (VF) and further explored the preventive factors of VL high-risk events. Methods The data were derived from China’s HIV/AIDS Comprehensive Response Information Management System. HIV infected patients who initiated or received ART in Guangxi between 2003 and 2019 were included. The contributions of VL after 6 months of ART to VF and AIDS-related death were analysed by Kaplan-Meier curves, log-rank tests and Cox regression analyses. Both descriptive analyses and bivariate logistic regression were employed to further explore the preventive factors related to VL high-risk events of VF. Results The cumulative rates of VF in the high low-level viremia group (high LLV) (χ2 = 18.45; P χ2 = 82.99; P 200 copies/ml after 6 months of ART. Compared with the VS group, the adjusted hazard risk was 7.221 (95% CI: 2.668; 19.547) in the high LLV group and 8.351 (95% CI: 4.253; 16.398) in the non-suppressed group. Compared with single patients, married or cohabiting (AOR = 0.591; 95% CI: 0.408, 0.856) and divorced or separated (AOR = 0.425, 95% CI: 0.207, 0.873) patients were negatively associated with VL high-risk events. So were patients acquired HIV homosexually (AOR = 0.572; 95% CI: 0.335, 0.978). However, patients who had ART modification were 1.728 times (95% CI: 1.093, 2.732) more likely to have VL high-risk events, and patients who used cotrimoxazole during ART were 1.843 times (95% CI: 1.271, 2.672) more likely to have VL high-risk events. Conclusions A VL greater than 200 copies/ml is a VL high-risk event for VF. Intervention measurements should be adopted to optimize the surveillance of ART in patients who are single or widowed, who have ART modification, and who use cotrimoxazole during ART.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aaa80e1d8bebb4e16baad537a2ff13c2Test
https://doaj.org/article/1827d45f60714d05831cb0580cfb1e8cTest

3

Quantitative analysis of internal components of the human crystalline lens during accommodation in adults

المؤلفون: Jinming Guo, Qiongfang Xu, Wei Chen, Ting Fu, Ping Wang, Anne Manyande, Hong Zhang, Zhiqi Chen, Xuebi Tian, Junming Wang, Yan Xiang, Chaohua Deng

المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
Scientific Reports

مصطلحات موضوعية: Adult, medicine.medical_specialty, clinical_medicine, Science, Posterior parietal cortex, 01 natural sciences, Article, 010309 optics, 03 medical and health sciences, 0302 clinical medicine, Ophthalmology, Cortex (anatomy), 0103 physical sciences, Lens, Crystalline, medicine, Image Processing, Computer-Assisted, Humans, Multidisciplinary, Lenticular nucleus, business.industry, Age Factors, Accommodation, Ocular, health, clinical-care, Healthy Volunteers, eye diseases, medicine.anatomical_structure, Preclinical research, Lens (anatomy), Anterior cortex, 030221 ophthalmology & optometry, Vertex (curve), Medicine, sense organs, Anatomy, business, Nucleus, Accommodation, Tomography, Optical Coherence

الوصف: Objectives. To quantitatively analyze changes in the inner components of the human crystalline lens during accommodation in adults. \ud Methods. Eyes of 23 subjects were sequentially examined using CASIA2 Optical Coherence Tomography under 0D, -3D and -6D accommodation states. The anterior chamber depth (ACD), anterior and posterior crystalline lens radius of the curvature (ALRC and PLRC) were obtained using built-in software. The lens thickness (LT), lenticular nucleus thickness (NT), anterior cortex thickness (ACT), posterior cortex thickness (PCT), anterior and posterior lenticular nucleus radius of the curvature (ANRC and PNRC), anterior and posterior lenticular nucleus vertex (ANV and PNV) were quantified manually with the Image-pro plus software.\ud Results. During accommodation, the ACD became significantly shallower and LT significantly increased. For changes in the lens, the ALRC decreased by an average magnitude （related to accommodative stimuli） 0.44 mm/D and PLRC decreased 0.09 mm/D. There was no difference for the ACT and PCT in different accommodation states. For lenticular nucleus response, NT increased on average by 30μm/D. Both the ANRC and PNRC decreased on average by 212 μm/D and 115 μm/D respectively. The ANV moved forward on average by 0.07mm under -3D accommodative stimuli and 0.16mm for -6D. However, there was no statistically significant difference between different accommodation states in the PNV movement.\ud Conclusion. Under accommodation stimulation, lens thickness changed mainly due to the lenticular nucleus, but not the cortex. For the lenticular nucleus, both the ANRC and PNRC decreased and ANRC changed the most. The anterior surface of the nucleus moved forward while the posterior surface of the nucleus moved backward but only slightly.\ud Keywords: crystalline lens, lenticular nucleus, lenticular cortex, accommodation

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34c3b50ba1021fbd6106a5388b0ca911Test
https://doaj.org/article/53835e4e1173458aab6a3431d62dfe90Test

4

A randomized phase-I pharmacokinetic trial comparing the potential biosimilar tocilizumab (QX003S) with the reference product (Actemra®) in Chinese healthy subjects

المؤلفون: Jingrui Liu, Yanhua Ding, Cuiyun Li, Min Wu, Jixuan Sun, Hong Zhang, Xiaoxue Zhu, Min Fang, Xiaojiao Li

المصدر: Annals of Medicine
article-version (VoR) Version of Record

الوصف: QX003S is a biosimilar candidate for the reference tocilizumab, Actemra®. We investigated the tolerance, variability, and pharmacokinetics (PK) of QX003S biosimilar in healthy Chinese male subjects. A randomised, double-blind, two-arm, parallel study was performed to examine the bioequivalence of QX003S (8 mg/kg) with that of Actemra® as a reference drug. QX003S (N = 40) and Actemra® (N = 40) groups exhibited similar PK properties. The inter-subject variability ranged from 14.95% to 18.78%. The 90% confidence intervals of the ratios for Cmax, AUC0–t andAUC0–∞ in both groups were within the range of 80–125%. After administration, the number of subjects who tested positive for anti-drug antibodies (ADA) in the QX003S group and Actemra® groups was 6 (14.3%) and 14 (34.1%), respectively. Adverse reactions occurred in 100% and 97.6% subjects in the QX003S and Actemra® groups, respectively. The most common adverse reactions were decrease in fibrinogen level and neutrophil and leukocyte counts. The PK characteristics and immunogenicity exhibited by QX003S were similar to that of the reference product, Actemra®. The safety profile was similar in the two treatment groups with mild-moderate adverse effects.Trial RegistrationThe trial is registered at Chinese Clinical Trial website (http://www.chinadrugtrials.org.cn/index.html#CTR20190002Test)Key pointsThis was the first clinical report of a new proposed tocilizumab biosimilar, QX003S.This phase-I randomized, controlled study compared pharmacokinetics, variability,immunogenicity, and safety of QX003S vs. the approved tocilizumab product (Actemra@).The results demonstrate bioequivalence between BAT1806 and the reference products (Actemra@), as well as comparable immunogenicity, safety and tolerability profiles. The trial is registered at Chinese Clinical Trial website (http://www.chinadrugtrials.org.cn/index.html#CTR20190002Test) This was the first clinical report of a new proposed tocilizumab biosimilar, QX003S. This phase-I randomized, controlled study compared pharmacokinetics, variability,immunogenicity, and safety of QX003S vs. the approved tocilizumab product (Actemra@). The results demonstrate bioequivalence between BAT1806 and the reference products (Actemra@), as well as comparable immunogenicity, safety and tolerability profiles.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86b13c7ca9712f07e3059aa6405788a8Test
http://europepmc.org/articles/PMC7919877Test

5

Evaluations of Clinical Utilization of Metagenomic Next-Generation Sequencing in Adults With Fever of Unknown Origin

المؤلفون: Zhang-fan Fu, Hao-cheng Zhang, Yi Zhang, Peng Cui, Yang Zhou, Hong-yu Wang, Ke Lin, Xian Zhou, Jing Wu, Hong-long Wu, Wen-hong Zhang, Jing-wen Ai

المصدر: Frontiers in Cellular and Infection Microbiology, Vol 11 (2022)
Frontiers in Cellular and Infection Microbiology

مصطلحات موضوعية: Adult, Microbiology (medical), etiology, Immunology, High-Throughput Nucleotide Sequencing, Sensitivity and Specificity, Microbiology, metagenomic next-generation sequencing, QR1-502, precision diagnose, Cellular and Infection Microbiology, Infectious Diseases, fever of unknown origin, Humans, Metagenome, Metagenomics, application, Original Research, Retrospective Studies

الوصف: IntroductionThe diagnosis of infection-caused fever of unknown origin (FUO) is still challenging, making it difficult for physicians to provide an early effective therapy. Therefore, a novel pathogen detection platform is needed. Metagenomic next-generation sequencing (mNGS) provides an unbiased, comprehensive technique for the sequence-based identification of pathogenic microbes, but the study of the diagnostic values of mNGS in FUO is still limited.MethodsIn a single-center retrospective cohort study, 175 FUO patients were enrolled, and clinical data were recorded and analyzed to compare mNGS with culture or traditional methods including as smears, serological tests, and nucleic acid amplification testing (NAAT) (traditional PCR, Xpert MTB/RIF, and Xpert MTB/RIF Ultra).ResultsThe blood mNGS could increase the overall rate of new organisms detected in infection-caused FUO by roughly 22.9% and 19.79% in comparison to culture (22/96 vs. 0/96; OR, ∞; p = 0.000) and conventional methods (19/96 vs. 3/96; OR, 6.333; p = 0.001), respectively. Bloodstream infection was among the largest group of those identified, and the blood mNGS could have a 38% improvement in the diagnosis rate compared to culture (19/50 vs. 0/50; OR, ∞; p = 0.000) and 32.0% compared to conventional methods (16/50 vs. 3/50; OR, 5.333; p = 0.004). Among the non-blood samples in infection-caused FUO, we observed that the overall diagnostic performance of mNGS in infectious disease was better than that of conventional methods by 20% (9/45 vs. 2/45; OR, 4.5; p = 0.065), and expectedly, the use of non-blood mNGS in non-bloodstream infection increased the diagnostic rate by 26.2% (8/32 vs. 0/32; OR, ∞; p = 0.008). According to 175 patients’ clinical decision-making, we found that the use of blood mNGS as the first-line investigation could effectively increase 10.9% of diagnosis rate of FUO compared to culture, and the strategy that the mNGS of suspected parts as the second-line test could further benefit infectious patients, improving the diagnosis rate of concurrent infection by 66.7% and 12.5% in non-bloodstream infection, respectively.ConclusionThe application of mNGS in the FUO had significantly higher diagnostic efficacy than culture or other conventional methods. In infection-caused FUO patients, application of blood mNGS as the first-line investigation and identification of samples from suspected infection sites as the second-line test could enhance the overall FUO diagnosis rate and serve as a promising optimized diagnostic protocol in the future.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43613403054a808e04f91df6f96a92a1Test
https://www.frontiersin.org/articles/10.3389/fcimb.2021.745156/fullTest

6

Poly-IgA Complexes and Disease Severity in IgA Nephropathy

المؤلفون: Dan Liu, Jicheng Lv, Xue Zhang, Manliu Wang, Xinfang Xie, Pan Liu, Jing Jin, Hong Zhang

المصدر: Clin J Am Soc Nephrol

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Adolescent, Epidemiology, medicine.drug_class, Kidney Glomerulus, Enzyme-Linked Immunosorbent Assay, Receptors, Fc, Critical Care and Intensive Care Medicine, Immune complex formation, Gastroenterology, Severity of Illness Index, Nephropathy, Young Adult, Interquartile range, Antigens, CD, Internal medicine, medicine, Humans, Serologic Tests, Glucocorticoids, Transplantation, business.industry, Area under the curve, Glomerulonephritis, Glomerulonephritis, IGA, Original Articles, Middle Aged, medicine.disease, Immune complex, Recombinant Proteins, Immunoglobulin A, Nephrology, Area Under Curve, Case-Control Studies, Corticosteroid, Female, business, Immunosorbents, Immunosuppressive Agents, Kidney disease, Glomerular Filtration Rate

الوصف: BACKGROUND AND OBJECTIVES: Poly-IgA immune complex formation and glomerular deposition play a key role in IgA nephropathy. Our study sought to develop a new methodology for one-step serologic detection of poly-IgA levels. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: A novel ELISA method using recombinant CD89 as a “capturing” probe was established for detecting poly-IgA immune complex in plasma. We applied semiquantitative measurements of these poly-IgA indices in patients recruited at Peking University First Hospital who had IgA nephropathy or other kidney disease types, as compared with healthy controls. The longitudinal trend of the poly-IgA index and the association with pathologic parameters and treatment responses were evaluated. Finally, we analyzed the molecular composition of poly-IgA complexes in patients by mass spectrometry. RESULTS: Recombinant CD89–mounted ELISA plates specifically captured plasma poly-IgA. The levels of poly-IgA immune complex (26.7 [interquartile range (IQR) 17.1–42.6] U/ml) in IgA nephropathy were significantly higher than those in healthy controls (15.5 [IQR 10.7–20.0] U/ml; P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::498ed99e98ea19336e02ba0ecdadd98dTest
https://europepmc.org/articles/PMC8729420Test/

7

The lateral center-edge angle as radiographic selection criteria for periacetabular osteotomy for developmental dysplasia of the hip in patients aged above 13 years

المؤلفون: Xin Pan, Hong Zhang, Kai Xiao, Dianzhong Luo, Daguang Zhang, Hui Cheng

المصدر: BMC Musculoskeletal Disorders, Vol 21, Iss 1, Pp 1-9 (2020)
BMC Musculoskeletal Disorders

مصطلحات موضوعية: Adult, medicine.medical_specialty, lcsh:Diseases of the musculoskeletal system, Sports medicine, Adolescent, Radiography, Osteoarthritis, 03 medical and health sciences, Femoral head, Young Adult, 0302 clinical medicine, Rheumatology, Internal medicine, medicine, Humans, Orthopedics and Sports Medicine, Tönnis OA classification, Hip Dislocation, Congenital, Femoral neck, Aged, Retrospective Studies, 030222 orthopedics, business.industry, Patient Selection, Acetabulum, 030229 sport sciences, Middle Aged, medicine.disease, Osteotomy, Lateral center-edge angle, medicine.anatomical_structure, Treatment Outcome, Orthopedic surgery, Periacetabular osteotomy, Hip Joint, lcsh:RC925-935, business, Nuclear medicine, Research Article, Developmental dysplasia of the hip

الوصف: Background This retrospective study sought to delineate the radiographic characteristics of DDH patients over 13 years of age and investigate whether the lateral center-edge angle (LCEA) could serve as radiographic selection criteria for periacetabular osteotomy. Methods We enrolled patients with Hartofilakidis type I DDH without dislocation who underwent periacetabular osteotomy between August 2009 and August 2012. LCEA, anterior central edge angle (ACEA), femoral neck-shaft angle (FNSA), Shenton line and Tönnis acetabular index (AI) were evaluated by anteroposterior and 65° false⁃profile pelvic X-ray radiographs in the standing position. Femoral neck anteversion angle (FNA), labral lesion, labral inversion and cartilage lesion were evaluated by direct magnetic resonance arthrography. DDH was categorized by LCEA into four grades (grade I: 10° ≤ LCEA Results Totally patients (274 hips) with a mean age of 27.3 years (range 13–47 years) were included. The mean LCEA was 3.5° (range: − 30° to 20°). Based on LCEA grades, grade I DDH was present in 104 hips, grade II in 40 hips, grade III in 76 hips, and grade IV in 54 hips. Based on Tönnis OA classification, 54.5% hips (150/274) were grade 0, 33.1% hips (91/274) grade 1, 8.4% hips (23/274) grade 2 and 4% hips (11/274) grade 3. Pearson correlation analysis showed a negative correlation between LCEA grade and Tönnis OA grades (r = 0.3987; P P P Conclusion The LCEA classification scheme offers a simple and practical approach to categorize the level of acetabulum coverage on the femoral head, hip deformity and characteristics of DDH. Our findings could provide clinically useful guidance for orthopedic surgeons in preparation for periacetabular osteotomy in DDH patients aged above 13 years.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ec3c9840648e893e744c595fa411b3cTest
http://link.springer.com/article/10.1186/s12891-020-03515-8Test

8

Impact of implementation of a breech clinic in a tertiary hospital

المؤلفون: Sophie Alexander, Caroline Daelemans, Sara Derisbourg, S. Amirgholami, An Vercoutere, Elena Costa, Axelle Pintiaux, Wei Hong Zhang, V. Bogne Kamdem, L. De Luca, Pierre Buekens, Yvon Englert

المصدر: BMC Pregnancy and Childbirth, Vol 20, Iss 1, Pp 1-12 (2020)
BMC Pregnancy and Childbirth
BMC pregnancy and childbirth, 20 (1

مصطلحات موضوعية: Obstétrique, Intrauterine growth restriction, Tertiary Care Centers, 0302 clinical medicine, Gynécologie, Belgium, Breech presentation, Pregnancy, Infant Mortality, Care pathway, 030212 general & internal medicine, Prospective Studies, Prospective cohort study, reproductive and urinary physiology, 030219 obstetrics & reproductive medicine, Obstetrics, Vaginal delivery, Incidence (epidemiology), Pregnancy Outcome, Obstetrics and Gynecology, Neonatal outcome, female genital diseases and pregnancy complications, Bioéthique, Female, Research Article, Adult, medicine.medical_specialty, Care-pathway, Déontologie, Reproductive medicine, lcsh:Gynecology and obstetrics, 03 medical and health sciences, Young Adult, medicine, Humans, Breech Presentation, lcsh:RG1-991, Retrospective Studies, Breech delivery, business.industry, Cesarean Section, Infant, Newborn, Infant, Breech clinic, medicine.disease, Delivery, Obstetric, Mode of delivery, Psychologie, Ethique, Apgar Score, business

الوصف: The incidence of breech presentation in single pregnancies at term is between three to 5 %. In order to support eligible women in their choice of mode of delivery, a dedicated breech clinic with a care pathway was developed in December 2015 in a tertiary referral centre in Brussels. The primary objective of this study was to evaluate the vaginal birth rate before and after the introduction of a dedicated breech clinic. The secondary objective was to compare the early neonatal outcomes before and after the breech clinic was introduced.
info:eu-repo/semantics/published

وصف الملف: 1 full-text file(s): application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64618b5461528941c97dcf21197577dbTest
http://link.springer.com/article/10.1186/s12884-020-03122-4Test

9

Effects of dexmedetomidine on stress hormones in patients undergoing cardiac valve replacement: a randomized controlled trial

المؤلفون: Hanhua Wu, Jinqing Tang, Hong Zhang, Jiamei Pan, Ming Han, Hui-jun Cai

المصدر: BMC Anesthesiology, Vol 20, Iss 1, Pp 1-9 (2020)
BMC Anesthesiology

الوصف: Background Stress response always occurs in cardiac valve replacement patients undergoing cardiopulmonary bypass (CPB). Methods 60 patients undergoing cardiac valve replacement were recruited and randomized into control and Dex groups. Dex group received 1.0 μg·kg-1 of Dex for 10 min intravenously before anesthesia, followed by 0.5 μg·kg-1·h-1 of Dex, steadily administered throughout the procedure. And controlled group received the identical velocity of saline as Dex group. Plasma level of cortisol (Cor), epinephrine (E), norepinephrine (NE), and serotonin (5-HT) were evaluated at four timepoints: Before administration (T0), sawn sternum (T1), end of extracorporeal circulation (T2), and 24 h post operation (T3). General data of operation and recovery such as heart rate (HR), mean arterial pressure (MAP), intraoperative bispectral index (BIS), and hospitalization time in the intensive care unit (ICU) were also compared. Results Increase of Cor, E, NE, and 5-HT for the Dex group was significant lesser than that in the control group (P Conclusions Dex reduces plasma Cor, E and NE elevations in patients after CPB, alleviates the stress reaction of the body, shortens the hospitalization time and ventilator support time in ICU, and plays a positive role in the rehabilitation of patients undergoing cardiac valve replacement. Trial registration China Clinical Trial Registry (No. ChiCTR-IPR-17010954) March 22rd, 2017.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95a8da94d6b954b18ef23a5722924c05Test
http://link.springer.com/article/10.1186/s12871-020-00993-0Test

10

Clinical characteristics of 194 cases of COVID-19 in Huanggang and Taian, China

المؤلفون: Xuejing Zhang, Qinghua Liu, Hong Zhang, Wenli Shang, Maochuan Zheng, Maokui Yue

المصدر: Infection

مصطلحات موضوعية: 0301 basic medicine, Male, ACE2, Gene Expression, medicine.disease_cause, Severity of Illness Index, Infectious Disease Incubation Period, 0302 clinical medicine, COVID-19 Testing, Sore throat, 030212 general & internal medicine, Huanggang, Coronavirus, medicine.diagnostic_test, Pharyngitis, General Medicine, Middle Aged, Prognosis, Novel coronavirus pneumonia, Infectious Diseases, Female, Angiotensin-Converting Enzyme 2, Abnormality, medicine.symptom, Coronavirus Infections, Microbiology (medical), Adult, medicine.medical_specialty, China, Coronavirus disease 2019 (COVID-19), Adolescent, Fever, 030106 microbiology, Pneumonia, Viral, Peptidyl-Dipeptidase A, Real-Time Polymerase Chain Reaction, 03 medical and health sciences, Betacoronavirus, Internal medicine, Severity of illness, medicine, Humans, Pandemics, Survival analysis, Aged, Retrospective Studies, Original Paper, Clinical characteristics, business.industry, Clinical Laboratory Techniques, SARS-CoV-2, Coronavirus disease-19 (COVID-19), Nucleic acid test, COVID-19, Retrospective cohort study, Survival Analysis, Cough, Taian, business, Tomography, X-Ray Computed, Biomarkers

الوصف: Purpose We aimed to report the clinical characteristics of 194 cases coronavirus disease-19 (COVID-19) in Huanggang, Hubei and Taian, Shandong. Methods We retrospectively investigated the clinical, laboratory characteristics and CT imaging of confirmed cases of COVID-19 from January 22 to February 28, 2020 in Huanggang Central Hospital and The Second Affiliated Hospital of Shandong First Medical University. Real time PCR was used to detect the new coronavirus in respiratory samples. Immunohistochemical staining was used to detect the expressions of ACE2 in tissues. Results Among the 194 patients infected with COVID-19, 108 patients were male, with a median age of 48.3 years. The average preclinical period was 7.44 day. Except for 37 severe or critically ill patients, the rest of the 157 patients exhibited mild or moderate symptoms. 190 (97.94%) patients were confirmed during the three times nucleic acid test. The main clinical symptom of the patients were fever, sore throat and cough, which accounted for 146 cases (75.26%), 98 (50.52%) and 86 cases (44.33%), respectively. 30 patients (15.46%) showed liver dysfunction. Imaging examination showed that 141 patients (72.68%) showed abnormal density shadow, while 53 cases (27.32%) had no obvious abnormality in the parenchyma of both lungs. Up to now, 109 cases have been discharged from the hospital, and 9 patients died. The ACE2 expression levels were up-regulated in patients of severe type and critically ill type. Conclusion Clinical symptoms, laboratory tests and CT imaging should be combined for comprehensive analysis to diagnose COVID-19. ACE2 may be the receptor of COVID-19. Electronic supplementary material The online version of this article (10.1007/s15010-020-01440-5) contains supplementary material, which is available to authorized users.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a619eb0b73f237241b85358e1f8ae11Test
http://europepmc.org/articles/PMC7211492Test

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