Phylogenetic and Demographic Characterization of Directed HIV-1 Transmission Using Deep Sequences from High-Risk and General Population Cohorts/Groups in Uganda
| العنوان: | Phylogenetic and Demographic Characterization of Directed HIV-1 Transmission Using Deep Sequences from High-Risk and General Population Cohorts/Groups in Uganda |
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| المؤلفون: | Oliver Ratmann, Alfred Ssekagiri, Ubaldo Bahemuka, Janet Seeley, Lucie Abeler-Dörner, Deogratius Ssemwanga, Deenan Pillay, Yunia Mayanja, Pontiano Kaleebu, Xiaoyue Xi, Christophe Fraser, Nicholas Bbosa, Tanya Golubchik |
| المساهمون: | Unit, MRC/UVRI and LSHTM Uganda Research, Bill & Melinda Gates Foundation |
| المصدر: | Viruses Volume 12 Issue 3 Viruses, Vol 12, Iss 3, p 331 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, Adolescent, Population, lcsh:QR1-502, HIV Infections, Disease, Genome, Viral, Biology, lcsh:Microbiology, Article, law.invention, 03 medical and health sciences, Young Adult, 0302 clinical medicine, law, Virology, key populations, Humans, Uganda, 030212 general & internal medicine, education, Phylogeny, education.field_of_study, Phylogenetic tree, human immunodeficiency virus, Risk of infection, Incidence (epidemiology), phylogenetic analysis, transmission, High-Throughput Nucleotide Sequencing, Genomics, Middle Aged, Models, Theoretical, Population sampling, deep sequences, 030104 developmental biology, Infectious Diseases, Transmission (mechanics), Hiv 1 transmission, Population Surveillance, HIV-1, Female, Algorithms, Demography, 0605 Microbiology |
| الوصف: | Across sub-Saharan Africa, key populations with elevated HIV-1 incidence and/or prevalence have been identified, but their contribution to disease spread remains unclear. We performed viral deep-sequence phylogenetic analyses to quantify transmission dynamics between the general population (GP), fisherfolk communities (FF), and women at high risk of infection and their clients (WHR) in central and southwestern Uganda. Between August 2014 and August 2017, 6185 HIV-1 positive individuals were enrolled in 3 GP and 10 FF communities, 3 WHR enrollment sites. A total of 2531 antiretroviral therapy (ART) naï ve participants with plasma viral load > 1000 copies/mL were deep-sequenced. One hundred and twenty-three transmission networks were reconstructed, including 105 phylogenetically highly supported source&ndash recipient pairs. Only one pair involved a WHR and male participant, suggesting that improved population sampling is needed to assess empirically the role of WHR to the transmission dynamics. More transmissions were observed from the GP communities to FF communities than vice versa, with an estimated flow ratio of 1.56 (95% CrI 0.68&ndash 3.72), indicating that fishing communities on Lake Victoria are not a net source of transmission flow to neighboring communities further inland. Men contributed disproportionally to HIV-1 transmission flow regardless of age, suggesting that prevention efforts need to better aid men to engage with and stay in care. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0d05830e07ee3807846fe51e44e3bb1Test http://europepmc.org/articles/PMC7150763Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b0d05830e07ee3807846fe51e44e3bb1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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