Beta-carbonic anhydrases play a role in fruiting body development and ascospore germination in the filamentous fungus Sordaria macrospora
| العنوان: | Beta-carbonic anhydrases play a role in fruiting body development and ascospore germination in the filamentous fungus Sordaria macrospora |
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| المؤلفون: | Skander Elleuche, Stefanie Pöggeler |
| المساهمون: | Herrera-Estrella, Alfredo |
| المصدر: | PLoS ONE, Vol 4, Iss 4, p e5177 (2009) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Hypha, Recombinant Fusion Proteins, Science, Developmental Biology/Microbial Growth and Development, Molecular Sequence Data, Mutant, Hyphae, Sordariales, Isozyme, Microbiology, Fungal Proteins, Sordaria macrospora, Developmental Biology/Molecular Development, 03 medical and health sciences, Gene Expression Regulation, Fungal, Carbonic anhydrase, Humans, Amino Acid Sequence, Fruiting Bodies, Fungal, Filamentous Fungus, b-Carbonic Anhydrases, Sordaria, Carbonic Anhydrases, 030304 developmental biology, 0303 health sciences, Fungal protein, Microbiology/Microbial Growth and Development, Multidisciplinary, Sequence Homology, Amino Acid, biology, Developmental Biology/Morphogenesis and Cell Biology, 030306 microbiology, Genetics and Genomics/Functional Genomics, Fatty Acids, Genetic Complementation Test, Carbon Dioxide, Spores, Fungal, biology.organism_classification, Genetics and Genomics/Gene Function, Isoenzymes, Phenotype, Biochemistry, Gene Knockdown Techniques, Ascospore, biology.protein, Medicine, Sequence Alignment, Research Article |
| الوصف: | Carbon dioxide (CO(2)) is among the most important gases for all organisms. Its reversible interconversion to bicarbonate (HCO(3) (-)) reaches equilibrium spontaneously, but slowly, and can be accelerated by a ubiquitous group of enzymes called carbonic anhydrases (CAs). These enzymes are grouped by their distinct structural features into alpha-, beta-, gamma-, delta- and zeta-classes. While physiological functions of mammalian, prokaryotic, plant and algal CAs have been extensively studied over the past years, the role of beta-CAs in yeasts and the human pathogen Cryptococcus neoformans has been elucidated only recently, and the function of CAs in multicellular filamentous ascomycetes is mostly unknown. To assess the role of CAs in the development of filamentous ascomycetes, the function of three genes, cas1, cas2 and cas3 (carbonic anhydrase of Sordaria) encoding beta-class carbonic anhydrases was characterized in the filamentous ascomycetous fungus Sordaria macrospora. Fluorescence microscopy was used to determine the localization of GFP- and DsRED-tagged CAs. While CAS1 and CAS3 are cytoplasmic enzymes, CAS2 is localized to the mitochondria. To assess the function of the three isoenzymes, we generated knock-out strains for all three cas genes (Deltacas1, Deltacas2, and Deltacas3) as well as all combinations of double mutants. No effect on vegetative growth, fruiting-body and ascospore development was seen in the single mutant strains lacking cas1 or cas3, while single mutant Deltacas2 was affected in vegetative growth, fruiting-body development and ascospore germination, and the double mutant strain Deltacas1/2 was completely sterile. Defects caused by the lack of cas2 could be partially complemented by elevated CO(2) levels or overexpression of cas1, cas3, or a non-mitochondrial cas2 variant. The results suggest that CAs are required for sexual reproduction in filamentous ascomycetes and that the multiplicity of isoforms results in redundancy of specific and non-specific functions. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66672f1e79aeadee701ae295de8ad66bTest https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19365544/?tool=EBITest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....66672f1e79aeadee701ae295de8ad66b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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