The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola
| العنوان: | The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola |
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| المؤلفون: | Adrien Gauthier, Sophie Trouvelot, Jani Kelloniemi, Patrick Frettinger, David Wendehenne, Xavier Daire, Jean-Marie Joubert, Alberto Ferrarini, Massimo Delledonne, Victor Flors, Benoit Poinssot |
| المساهمون: | Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Interactions Arbres-Microorganismes (IAM), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratoires Goëmar, University of Verona (UNIVR), Departamento de Ciencias Agrarias y del Medio Natural, Area de Fisiologia Vegetal, Universitat Jaume I, Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Università degli Studi di Verona |
| المصدر: | PLoS ONE PLoS ONE, Public Library of Science, 2018, 13 (3), pp.e0194327. ⟨10.1371/journal.pone.0194327⟩ PLoS ONE, Vol 13, Iss 3, p e0194327 (2018) |
| بيانات النشر: | Public Library of Science, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, beta-Glucans, lcsh:Medicine, Crops, Cyclopentanes, 01 natural sciences, Biochemistry, Fruits, Agricultural Production, Integrated Control, Gene Expression Regulation, Plant, Stress, Physiological, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Plant Immunity, Vitis, Oxylipins, lcsh:Science, Biology, Glucans, ComputingMilieux_MISCELLANEOUS, Disease Resistance, Plant Diseases, 2. Zero hunger, Multidisciplinary, Cell Death, Plant Biochemistry, lcsh:R, 010401 analytical chemistry, Cell Membrane, Crop Diseases, Correction, Agriculture, Organic Farming, Sustainable Agriculture, 0104 chemical sciences, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Oomycetes, lcsh:Q, Pest Control, Agrochemicals, Reactive Oxygen Species, Salicylic Acid, Transcriptome, 010606 plant biology & botany, Research Article, Signal Transduction |
| الوصف: | Grapevine (Vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. Chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. The β-glucan laminarin (Lam) and its sulfated derivative (PS3) have been previously demonstrated to induce resistance in grapevine against downy mildew (Plasmopara viticola). However, if Lam elicits classical grapevine defenses such as oxidative burst, pathogenesis-related (PR)-proteins and phytoalexin production, PS3 triggered grapevine resistance via a poorly understood priming phenomenon. The aim of this study was to identify the molecular mechanisms of the PS3-induced resistance. For this purpose we studied i) the signaling events and transcriptome reprogramming triggered by PS3 treatment on uninfected grapevine, ii) grapevine immune responses primed by PS3 during P. viticola infection. Our results showed that i) PS3 was unable to elicit reactive oxygen species (ROS) production, cytosolic Ca(2+) concentration variations, mitogen-activated protein kinase (MAPK) activation but triggered a long lasting plasma membrane depolarization in grapevine cells, ii) PS3 and Lam shared a common stress-responsive transcriptome profile that partly overlapped the salicylate- (SA) and jasmonate-(JA)-dependent ones. After P. viticola inoculation, PS3 specifically primed the SA- and ROS-dependent defense pathways leading to grapevine induced resistance against this biotroph. Interestingly pharmacological approaches suggested that the plasma membrane depolarization and the downstream ROS production are key events of the PS3-induced resistance. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f546e119acd9996b5efbb203a9f462aTest http://europepmc.org/articles/PMC5843334Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9f546e119acd9996b5efbb203a9f462a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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