De Novo Transcriptome Assembly and Characterization of Lithospermum officinale to Discover Putative Genes Involved in Specialized Metabolites Biosynthesis
| العنوان: | De Novo Transcriptome Assembly and Characterization of Lithospermum officinale to Discover Putative Genes Involved in Specialized Metabolites Biosynthesis |
|---|---|
| المؤلفون: | Hideyuki Suzuki, Michimi Nakamura, Amit Rai, Kazuki Saito, Yohei Shimizu, Megha Rai, Mami Yamazaki, Taiki Nakaya |
| المصدر: | Planta Medica. 84:920-934 |
| بيانات النشر: | Georg Thieme Verlag KG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Candidate gene, De novo transcriptome assembly, Pharmaceutical Science, Computational biology, Biology, Depsides, Plant Roots, Genome, Analytical Chemistry, Transcriptome, 03 medical and health sciences, Drug Discovery, Gene expression, Metabolome, KEGG, Gene, Benzofurans, Pharmacology, Plant Stems, Lithospermum, Organic Chemistry, Biosynthetic Pathways, Plant Leaves, Gene Ontology, 030104 developmental biology, Complementary and alternative medicine, Molecular Medicine, Chlorogenic Acid, Naphthoquinones |
| الوصف: | Lithospermum officinale is a valuable source of bioactive metabolites with medicinal and industrial values. However, little is known about genes involved in the biosynthesis of these metabolites, primarily due to the lack of genome or transcriptome resources. This study presents the first effort to establish and characterize de novo transcriptome assembly resource for L. officinale and expression analysis for three of its tissues, namely leaf, stem, and root. Using over 4Gbps of RNA-sequencing datasets, we obtained de novo transcriptome assembly of L. officinale, consisting of 77,047 unigenes with assembly N50 value as 1524 bps. Based on transcriptome annotation and functional classification, 52,766 unigenes were assigned with putative genes functions, gene ontology terms, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. KEGG pathway and gene ontology enrichment analysis using highly expressed unigenes across three tissues and targeted metabolome analysis showed active secondary metabolic processes enriched specifically in the root of L. officinale. Using co-expression analysis, we also identified 20 and 48 unigenes representing different enzymes of lithospermic/chlorogenic acid and shikonin biosynthesis pathways, respectively. We further identified 15 candidate unigenes annotated as cytochrome P450 with the highest expression in the root of L. officinale as novel genes with a role in key biochemical reactions toward shikonin biosynthesis. Thus, through this study, we not only generated a high-quality genomic resource for L. officinale but also propose candidate genes to be involved in shikonin biosynthesis pathways for further functional characterization. |
| تدمد: | 1439-0221 0032-0943 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d26dd9ae3ad7d883506981a6143609fdTest https://doi.org/10.1055/a-0630-5925Test |
| رقم الانضمام: | edsair.doi.dedup.....d26dd9ae3ad7d883506981a6143609fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390221 00320943 |
|---|