Inhibition of nitric oxide synthase expression in activated microglia and peroxynitrite scavenging activity byOpuntia ficus indica var.saboten
| العنوان: | Inhibition of nitric oxide synthase expression in activated microglia and peroxynitrite scavenging activity byOpuntia ficus indica var.saboten |
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| المؤلفون: | Hyo Jin Lim, Chang-Kyun Han, Jeong Hoon Yoon, Wie-Jong Kwak, Changbae Jin, Jae-Ha Ryu, Ming Hong Lee, Jae Yeon Kim, Tae Hee Kim |
| المصدر: | Phytotherapy Research. 20:742-747 |
| بيانات النشر: | Wiley, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Gene Expression, Nitric Oxide Synthase Type I, Nitric Oxide, Neuroprotection, Cell Line, Nitric oxide, Mice, chemistry.chemical_compound, Peroxynitrous Acid, medicine, Animals, Pharmacology, chemistry.chemical_classification, Reactive oxygen species, biology, Microglia, Plant Extracts, Superoxide, Neurotoxicity, Opuntia, medicine.disease, Molecular biology, Nitric oxide synthase, Neuroprotective Agents, medicine.anatomical_structure, chemistry, Biochemistry, biology.protein, I-kappa B Proteins, Peroxynitrite |
| الوصف: | Activated microglia by neuronal injury or inflammatory stimulation overproduce nitric oxide (NO) by inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) such as superoxide anion, resulting in neurodegenerative diseases. The toxic peroxynitrite (ONOO-), the reaction product of NO and superoxide anion further contributes to oxidative neurotoxicity. A butanol fraction obtained from 50% ethanol extracts of Opuntia ficus indica var. saboten (Cactaceae) stem (SK OFB901) and its hydrolysis product (SK OFB901H) inhibited the production of NO in LPS-activated microglia in a dose dependent manner (IC50 15.9, 4.2 microg/mL, respectively). They also suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells at higher than 30 microg/mL as observed by western blot analysis and RT-PCR experiment. They also inhibited the degradation of I-kappaB-alpha in activated microglia. Moreover, they showed strong activity of peroxynitrite scavenging in a cell free bioassay system. These results imply that Opuntia ficus indica may have neuroprotective activity through the inhibition of NO production by activated microglial cells and peroxynitrite scavenging activity. |
| تدمد: | 1099-1573 0951-418X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ab640f0d4c3cfa3b1e2a68cc968d4b3Test https://doi.org/10.1002/ptr.1942Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....2ab640f0d4c3cfa3b1e2a68cc968d4b3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10991573 0951418X |
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