CCDC134 is down-regulated in gastric cancer and its silencing promotes cell migration and invasion of GES-1 and AGS cells via the MAPK pathway
| العنوان: | CCDC134 is down-regulated in gastric cancer and its silencing promotes cell migration and invasion of GES-1 and AGS cells via the MAPK pathway |
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| المؤلفون: | Mei Zhao, Jia Wang, Qing Luo, Jianli Sang, Jialing Zhong, Jian Liu, Xinghua Yuan, Changzhi Huang, Yiming Ma, Mei Yang |
| المصدر: | Molecular and Cellular Biochemistry. 372:1-8 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | MAPK/ERK pathway, MAP Kinase Kinase 4, MAP Kinase Signaling System, p38 mitogen-activated protein kinases, Clinical Biochemistry, Down-Regulation, Biology, p38 Mitogen-Activated Protein Kinases, Cell Line, Malignant transformation, Cell Movement, Stomach Neoplasms, medicine, Humans, Gene silencing, Neoplasm Invasiveness, Phosphorylation, RNA, Small Interfering, Nuclear protein, Extracellular Signal-Regulated MAP Kinases, Molecular Biology, JNK Mitogen-Activated Protein Kinases, Membrane Proteins, Nuclear Proteins, Cancer, Cell migration, Cell Biology, General Medicine, medicine.disease, Cell biology, Matrix Metalloproteinase 9, Tissue Array Analysis, Cell culture, Gene Knockdown Techniques, Matrix Metalloproteinase 2, RNA Interference, Protein Processing, Post-Translational |
| الوصف: | CCDC134 (coiled coil domain containing 134), a novel secretory protein, acts as an inhibitor of Erk1/2 and JNK/SAPK pathways. However, the role of CCDC134 in cancer development is still lacking. In this study, we found that CCDC134 expression significantly reduced in gastric cancer tissues compared with normal tissues (P < 0.001) and lesion tissues (P < 0.001). But no statistically significant difference was observed between normal and lesion tissues (P = 0.842). In vitro transient transfection of CCDC134-specific siRNA significantly promoted the migration and invasion of both the normal gastric epithelial cell line GES-1 and gastric cancer cell line AGS cells. Further analysis revealed that the attenuated expression of CCDC134 promoted the activation of Erk1/2 and JNK/SAPK, but had no effect on p38. The activation of Erk1/2 and JNK/SAPK was required for CCDC134-mediated migration and invasion. Besides, CCDC134-RNAi could induce the expression of MMP-2 and MMP-9, which are key molecules involved in regulating cell migration and invasion. Therefore, CCDC134 may be a candidate biomarker for malignant transformation. It plays a role in regulation of cell migration and invasion, and could be a therapeutic target of gastric cancer. |
| تدمد: | 1573-4919 0300-8177 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb1fe545788fcd96fb26f31f1db0d538Test https://doi.org/10.1007/s11010-012-1418-4Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....bb1fe545788fcd96fb26f31f1db0d538 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734919 03008177 |
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