Enhanced Bruton’s tyrosine kinase activity in the kidney of patients with IgA nephropathy
| العنوان: | Enhanced Bruton’s tyrosine kinase activity in the kidney of patients with IgA nephropathy |
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| المؤلفون: | Jie Wei, Xiang-Ming Qi, Yan Wang, Yong-Gui Wu |
| المصدر: | International Urology and Nephrology |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Nephrology, medicine.medical_specialty, Macrophage, Urology, Lupus nephritis, urologic and male genital diseases, Nephropathy, Katafuchi semi-quantitative score, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Bruton’s tyrosine kinase, Internal medicine, Agammaglobulinaemia Tyrosine Kinase, medicine, Humans, Nephrology - Original Paper, Bruton's tyrosine kinase, Creatinine, Kidney, biology, business.industry, Macrophages, Monocyte, Oxford classification, Interleukin, Glomerulonephritis, IGA, IgA nephropathy, Middle Aged, medicine.disease, NF-κb, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, chemistry, biology.protein, Female, business, 030215 immunology |
| الوصف: | Purpose Bruton’s tyrosine kinase (BTK) is a vital biological molecule that contributes to immune regulation. Previous studies have showed that BTK can be detected in patients with lupus nephritis and rheumatoid arthritis. However, the role of BTK in IgA nephropathy (IgAN) has not yet been elucidated. The purpose of this research was to investigate the role of BTK activation in macrophages in IgAN. Methods Peripheral blood and renal tissue samples were collected from 63 patients with IgAN, and peritumoral normal tissues were collected from 20 patients after surgical resection of renal tumor for use as control. Additionally, 20 healthy volunteers were recruited as control. The levels of BTK, CD68, phosphorylated BTK (pBTK), phosphorylated NF-κB (p-NF-κB p65), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and monocyte chemotactic protein (MCP)-1 were measured by immunohistochemistry (IHC), real-time polymerase chain reaction (RT-PCR), western blotting, and enzyme-linked immunosorbent assay (ELISA). Results Compared to peritumoral normal tissues, the expression levels of CD68 and BTK were significantly increased in IgAN group (p p r = 0.580, 0.637 and 0.442, 0.489, respectively, p p r = 0.456 and 0.453, respectively, p r = 0.357, p p Conclusion The results of this study indicate that activation of BTK in macrophages may play an important role in promoting the progression of renal inflammation in IgAN. |
| تدمد: | 1573-2584 0301-1623 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1de308613139292597a51e071801fbd3Test https://doi.org/10.1007/s11255-020-02733-2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1de308613139292597a51e071801fbd3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15732584 03011623 |
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