Local immune response in bladder pain syndrome/interstitial cystitis ESSIC type 3C
| العنوان: | Local immune response in bladder pain syndrome/interstitial cystitis ESSIC type 3C |
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| المؤلفون: | Marianne Gamper, JoEllen Welter, Carlo Moll, Volker Viereck, René Moser, Jakob Eberhard, Jochen Binder |
| المصدر: | International Urogynecology Journal |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Adult, CD4-Positive T-Lymphocytes, medicine.medical_specialty, Urology, Urinary system, Urinary Bladder, Cystitis, Interstitial, Gene Expression, Keratin-20, Lymphocyte Activation, urologic and male genital diseases, Immunoglobulin G, Bladder pain syndrome/interstitial cystitis, Obstetrics and Gynaecology, medicine, Humans, CTLA-4 Antigen, Immune response, Urothelium, Aged, Uroplakin Ib, B-Lymphocytes, Uroplakin III, Urinary bladder, medicine.diagnostic_test, biology, Urinary Bladder, Overactive, business.industry, Overactive bladder, Keratin 20, Obstetrics and Gynecology, Interstitial cystitis, Diagnostic markers, Cystoscopy, Middle Aged, Antigens, CD20, medicine.disease, Immunoglobulin A, medicine.anatomical_structure, biology.protein, Original Article, Female, business, Biomarkers, CD79 Antigens |
| الوصف: | Introduction and hypothesis Bladder pain syndrome/interstitial cystitis (BPS/IC) is identified based on subjective symptoms which lead to heterogeneous patient populations. Previous studies using gene expression arrays for BPS/IC with Hunner’s lesions [European Society for the Study of Interstitial Cystitis (ESSIC) type 3C], a subtype of the condition discernible by cystoscopy, have revealed characteristic immune responses and urothelial abnormalities. This current study aimed to further characterize this subtype using a gene expression panel. We hypothesized that B-cell activation with high levels of urinary antibody concentration would be found. Methods Cold-cup bladder biopsies, catheterized urine and blood were collected from 15 BPS/IC ESSIC type 3C patients, 11 non-inflammatory overactive bladder (OAB) patients and eight healthy controls. Gene expression in biopsies was quantified by real-time quantitative polymerase chain reaction (RT-qPCR), immunohistochemistry was performed on bladder tissue and urinary immunoglobulins G and A were quantified by enzyme-linked immunosorbent assay. Statistical analyses included the Kruskal-Wallis test for non-parametric data and post hoc tests identified differences between groups. Results High expression of T- and B-cell markers (CTLA4, CD20, CD79A, IGH@), low expression of urothelial markers (KRT20, UPK1B, UPK3A), focal lymphoid aggregates in the submucosa and high immunoglobulin concentration in urine were found exclusively in BPS/IC ESSIC type 3C patients. Results for OAB were in intermediate ranges between the other two groups and UPK1B even reached significantly lower expression when compared to healthy controls. Conclusions BPS/IC ESSIC type 3C is characterized by a local adaptive immune response with elevated urinary antibody concentrations. Quantification of urinary immunoglobulin levels could be used for a non-invasive diagnosis of BPS/IC ESSIC type 3C. |
| تدمد: | 1433-3023 0937-3462 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39adb5ce865a3bcfc609e55128d8b273Test https://doi.org/10.1007/s00192-013-2112-0Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....39adb5ce865a3bcfc609e55128d8b273 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14333023 09373462 |
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