Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2
| العنوان: | Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2 |
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| المؤلفون: | Robert Freund, Dorte B. Bekker-Jensen, Goda Snieckute, Simon Bekker-Jensen, Christopher Tiedje, Jesper V. Olsen, Alexey Kotlyarov, Martin R Turner, Matthias Gaestel, Manoj B. Menon, Manuel D. Díaz-Muñoz, Philipp Trulley |
| المصدر: | Cell Reports, Vol 27, Iss 10, Pp 2859-2870.e6 (2019) Trulley, P, Snieckute, G, Bekker-Jensen, D, Menon, M B, Freund, R, Kotlyarov, A, Olsen, J V, Diaz-Muñoz, M D, Turner, M, Bekker-Jensen, S, Gaestel, M & Tiedje, C 2019, ' Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2 ', Cell Reports, vol. 27, no. 10, pp. 2859-2870.e6 . https://doi.org/10.1016/j.celrep.2019.05.024Test |
| بيانات النشر: | Elsevier, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Gene isoform, Untranslated region, Five prime untranslated region, MAP Kinase Signaling System, Protein Serine-Threonine Kinases, p38 Mitogen-Activated Protein Kinases, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Mice, 0302 clinical medicine, Eukaryotic translation, Animals, Humans, Protein Isoforms, RNA, Messenger, Phosphorylation, RNA, Small Interfering, Protein kinase A, Peptide Chain Initiation, Translational, lcsh:QH301-705.5, Heat-Shock Proteins, Regulation of gene expression, Mice, Knockout, Chemistry, Intracellular Signaling Peptides and Proteins, EIF4A1, RNA Helicase A, Cell biology, Mice, Inbred C57BL, 030104 developmental biology, HEK293 Cells, RAW 264.7 Cells, Gene Expression Regulation, lcsh:Biology (General), 030217 neurology & neurosurgery, RNA Helicases, Molecular Chaperones |
| الوصف: | Summary: Alternative translation is an important mechanism of post-transcriptional gene regulation leading to the expression of different protein isoforms originating from the same mRNA. Here, we describe an abundant long isoform of the stress/p38MAPK-activated protein kinase MK2. This isoform is constitutively translated from an alternative CUG translation initiation start site located in the 5′ UTR of its mRNA. The RNA helicase eIF4A1 is needed to ensure translation of the long and the known short isoforms of MK2, of which the molecular properties were determined. Only the short isoform phosphorylated Hsp27 in vivo, supported migration and stress-induced immediate early gene (IEG) expression. Interaction profiling revealed short-isoform-specific binding partners that were associated with migration. In contrast, the long isoform contains at least one additional phosphorylatable serine in its unique N terminus. In sum, our data reveal a longer isoform of MK2 with distinct physiological properties. : In the present study, Trulley et al. identify a N-terminal extended long isoform of the stress-activated protein kinase MK2 that constitutively arises from alternative translation initiation within the known MK2 mRNA. The long isoform has unique and shared molecular properties, compared to the canonical short isoform. Keywords: ribosome footprinting, alternative translation initiation, 5′ UTR, eIF4A1, MAPKAPK2 |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2211-1247 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34582960dd0ab6e715848184a0566225Test http://www.sciencedirect.com/science/article/pii/S2211124719306357Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....34582960dd0ab6e715848184a0566225 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22111247 |
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