On the Regenerative Capacity of Human Skeletal Muscle
| العنوان: | On the Regenerative Capacity of Human Skeletal Muscle |
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| المؤلفون: | Ulrich Knauf, Rustam R. Mundegar, Stefan Zimmermann, Anton Wernig, Uwe M. Martens, Oliver Högemeier, Margit Zweyer, Ralf Schäfer |
| المصدر: | Artificial Organs. 29:192-198 |
| بيانات النشر: | Wiley, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Adult, Senescence, Telomerase, Adolescent, Population, Biomedical Engineering, Medicine (miscellaneous), Enzyme-Linked Immunosorbent Assay, Bioengineering, Biology, Biomaterials, medicine, Humans, Regeneration, Myocyte, Child, Muscle, Skeletal, education, Cells, Cultured, Cellular Senescence, Aged, Genetics, education.field_of_study, Infant, Skeletal muscle, General Medicine, Middle Aged, Telomere, Immunohistochemistry, Cell biology, medicine.anatomical_structure, Child, Preschool, Stem cell, Cell aging |
| الوصف: | The proliferative capacity of organotypic muscle stem cells, the satellite cells, from nine healthy human donors aged between 2 and 78 years was investigated. There was a loss in proliferative capacity with age, but the oldest donors (76, 78 years) would still be able to replace their musculature several times. Depending on frequency of desmin-positive (i.e., myogenic) cells during prolonged expansion, myoblast cultures could be designated as stable or unstable. There was a weak correlation between mean telomere lengths (estimated by flow-FISH) and remaining mean population doublings until senescence. A bimodal distribution of mean telomere lengths was apparent in both stable and unstable myoblast cultures and could be assigned to populations of differently sized cells. Furthermore, due to the presence of nonmyogenic cells with longer telomeres, unstable cultures tended to show an increasing rather than decreasing mean telomeric length on expansion. Bimodal distributions in myoblast cultures could be due to hitherto undefined myoblast populations. |
| تدمد: | 1525-1594 0160-564X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e974caf63d19a12ed157ff7496fdedaeTest https://doi.org/10.1111/j.1525-1594.2005.29033.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....e974caf63d19a12ed157ff7496fdedae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15251594 0160564X |
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