Programmed death-1 expression and regulatory T cells increase in the Intestinal mucosa of cytomegalovirus colitis in patients with HIV/AIDS
| العنوان: | Programmed death-1 expression and regulatory T cells increase in the Intestinal mucosa of cytomegalovirus colitis in patients with HIV/AIDS |
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| المؤلفون: | Lei Sun, Ping Li, Kun Yang, Liang Zhang, Jiamin Chen, Peng Wang, Jiang Xiao, Li-ming Qi, Hongxin Zhao |
| المصدر: | AIDS Research and Therapy AIDS Research and Therapy, Vol 17, Iss 1, Pp 1-8 (2020) |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | lcsh:Immunologic diseases. Allergy, 0301 basic medicine, Cytomegalovirus, Cytomegalovirus colitis, HIV Infections, medicine.disease_cause, T-Lymphocytes, Regulatory, 03 medical and health sciences, 0302 clinical medicine, Intestinal mucosa, Virology, PD-1, Humans, Medicine, CMV colitis, Pharmacology (medical), 030212 general & internal medicine, Colitis, Regulatory T cells (Tregs), business.industry, Research, virus diseases, FOXP3, T lymphocyte, medicine.disease, 030112 virology, Giant cell, Cytomegalovirus Infections, Immunology, HIV/AIDS, Molecular Medicine, lcsh:RC581-607, business, CD8 |
| الوصف: | Background Cytomegalovirus (CMV) is among the most common opportunistic infections identified in patients with HIV/AIDS. CMV often targets the colon in such patients. However, the role of regulatory T cells (Tregs) and Programmed death-1 (PD-1) in intestinal CMV infection is unclear. In this study, we evaluate the expression of programmed death -1 (PD-1) and its association with regulatory T cells (Tregs) in patients with HIV/AIDS having CMV colitis. Methods CMV was detected in the intestinal mucosal biopsy samples via nucleic acid in situ hybridization. PD-1, CD4, CD8, and Treg-specific marker as well as the winged-helix transcription factor and forkhead box P3 (FoxP3) were detected by immunohistochemical methods. Results Intestinal CMV diease was identified in 20 out of 195 patients with HIV/AIDS enrolled in our study. CMV was diagnosed microscopically by the presence of giant cell inclusion bodies in epithelial cells, histiocytes, and fibroblasts. Levels of immunoreactive PD-1 detected in mucosal biopsies from patients with HIV/AIDS having CMV colitis were significantly higher than CMV-negative control group (p = 0.023). FoxP3+ cells were detected in the CMV colitis group slight more than that in the control group. CD4+ T lymphocyte counts in the peripheral blood and intestinal mucosal biopsies from CMV colitis group were all notably decreased compared with those with control group (p p = 0.016). CD8+T lymphocyte counts in peripheral blood and intestinal mucosa were slightly lower than those in the control group, although the differences were not statistically significant. Conclusions CMV colitis with HIV/AIDS is associated with significant changes in T lymphocyte populations. These findings may have important implications for disease pathogenesis and progression. |
| تدمد: | 1742-6405 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0433597df1b360d0f30b4598336561a4Test https://doi.org/10.1186/s12981-020-00315-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0433597df1b360d0f30b4598336561a4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17426405 |
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