دورية أكاديمية
Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders
| العنوان: | Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders |
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| المؤلفون: | Monchaud, David, Allain, C., Bertrand, Hélène, Smargiasso, Nicolas, Rosu, Frédéric, Gabelica, Valérie, De Cian, Anne, Mergny, Jean-Louis, Teulade-Fichou, Marie-Paule |
| المساهمون: | Giga-Systems Biology and Chemical Biology - ULiège |
| المصدر: | Biochimie, 90 (8), 1207-1223 (2008) |
| بيانات النشر: | Elsevier, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | G-quadruplex, thiazole orange, fluorescence, noncovalent, mass spectrometry, DNA, Life sciences, Biochemistry, biophysics & molecular biology, Physical, chemical, mathematical & earth Sciences, Chemistry, Sciences du vivant, Biochimie, biophysique & biologie moléculaire, Physique, chimie, mathématiques & sciences de la terre, Chimie |
| الوصف: | We report here the details of G4-FID (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex DNA binding affinity and quadruplex- over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex and duplex-DNA matrices. The original protocol was tested using various quadruplex andduplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metalloorganic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501Test article |
| اللغة: | English |
| العلاقة: | urn:issn:0300-9084; urn:issn:1638-6183 |
| DOI: | 10.1016/j.biochi.2008.02.019 |
| الوصول الحر: | https://orbi.uliege.be/handle/2268/612Test |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2Test info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsorb.612 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1016/j.biochi.2008.02.019 |
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