مورد إلكتروني
A Positive Regulatory Feedback Loop between EKLF/KLF1 and TAL1/SCL Sustaining the Erythropoiesis.
| العنوان: | A Positive Regulatory Feedback Loop between EKLF/KLF1 and TAL1/SCL Sustaining the Erythropoiesis. |
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| المؤلفون: | Hung, Chun-Hao |
| المصدر: | International journal of molecular sciences; vol 22, iss 15, 8024; 1422-0067 |
| بيانات النشر: | eScholarship, University of California 2021-07-01 |
| تفاصيل مُضافة: | Hung, Chun-Hao Lee, Tung-Liang Huang, Anna Yu-Szu Yang, Kang-Chung Shyu, Yu-Chiau Wen, Shau-Ching Lu, Mu-Jie Yuan, Shinsheng Shen, Che-Kun James |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The erythroid Krüppel-like factor EKLF/KLF1 is a hematopoietic transcription factor binding to the CACCC DNA motif and participating in the regulation of erythroid differentiation. With combined use of microarray-based gene expression profiling and the promoter-based ChIP-chip assay of E14.5 fetal liver cells from wild type (WT) and EKLF-knockout (Eklf-/-) mouse embryos, we identified the pathways and direct target genes activated or repressed by EKLF. This genome-wide study together with the molecular/cellular analysis of the mouse erythroleukemic cells (MEL) indicate that among the downstream direct target genes of EKLF is Tal1/Scl. Tal1/Scl encodes another DNA-binding hematopoietic transcription factor TAL1/SCL, known to be an Eklf activator and essential for definitive erythroid differentiation. Further identification of the authentic Tal gene promoter in combination with the in vivo genomic footprinting approach and DNA reporter assay demonstrate that EKLF activates the Tal gene through binding to a specific CACCC motif located in its promoter. These data establish the existence of a previously unknow positive regulatory feedback loop between two DNA-binding hematopoietic transcription factors, which sustains mammalian erythropoiesis. |
| مصطلحات الفهرس: | Liver, Fetus, Animals, Mice, Knockout, Erythropoiesis, Hematopoiesis, Extramedullary, Response Elements, Kruppel-Like Transcription Factors, T-Cell Acute Lymphocytic Leukemia Protein 1, EKLF/KLF1, TAL1/SCL, direct target genes, erythroid differentiation, gene knockout, genomic footprinting of Tal1 promoter, global gene expression profiling, transcriptional regulation, Biotechnology, Stem Cell Research, Hematology, Human Genome, Genetics, Stem Cell Research - Nonembryonic - Non-Human, 1.1 Normal biological development and functioning, Underpinning research, Blood, Other Chemical Sciences, Other Biological Sciences, Chemical Physics, article |
| URL: | |
| الإتاحة: | Open access content. Open access content public |
| ملاحظة: | application/pdf International journal of molecular sciences vol 22, iss 15, 8024 1422-0067 |
| أرقام أخرى: | CDLER oai:escholarship.org:ark:/13030/qt24b9q2zn qt24b9q2zn https://escholarship.org/uc/item/24b9q2znTest https://escholarship.orgTest/ 1391593839 |
| المصدر المساهم: | UC MASS DIGITIZATION From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1391593839 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |