مورد إلكتروني
A Pseudomonas aeruginosa toxin that hijacks the host ubiquitin proteolytic system
| العنوان: | A Pseudomonas aeruginosa toxin that hijacks the host ubiquitin proteolytic system |
|---|---|
| بيانات النشر: | Public Library of Science 2011-03-01 |
| تفاصيل مُضافة: | Bomberger, Jennifer M. Ye, Siying MacEachran, Daniel P. Koeppen, Katja Barnaby, Roxanna L. O'Toole, George A. Stanton, Bruce A. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen chronically infecting the lungs of patients with chronic obstructive pulmonary disease (COPD), pneumonia, cystic fibrosis (CF), and bronchiectasis. Cif (PA2934), a bacterial toxin secreted in outer membrane vesicles (OMV) by P. aeruginosa, reduces CFTR-mediated chloride secretion by human airway epithelial cells, a key driving force for mucociliary clearance. The aim of this study was to investigate the mechanism whereby Cif reduces CFTR-mediated chloride secretion. Cif redirected endocytosed CFTR from recycling endosomes to lysosomes by stabilizing an inhibitory effect of G3BP1 on the deubiquitinating enzyme (DUB), USP10, thereby reducing USP10-mediated deubiquitination of CFTR and increasing the degradation of CFTR in lysosomes. This is the first example of a bacterial toxin that regulates the activity of a host DUB. These data suggest that the ability of P. aeruginosa to chronically infect the lungs of patients with COPD, pneumonia, CF, and bronchiectasis is due in part to the secretion of OMV containing Cif, which inhibits CFTR-mediated chloride secretion and thereby reduces the mucociliary clearance of pathogens. |
| مصطلحات الفهرس: | bacterial toxin, bacterial toxin Cif, binding protein, chloride, enzyme, protein USP10, Ras GAP SH3 domain binding protein 1, small interfering RNA, transmembrane conductance regulator, ubiquitin, unclassified drug, Journal Article |
| URL: | issn: 1553-7366 issn: 1553-7374 PL o S pathogens |
| الإتاحة: | Open access content. Open access content 2011, The Authors |
| ملاحظة: | 13 p. English |
| أرقام أخرى: | LD0 oai:dro.deakin.edu.au:DU:30040950 945709486 |
| المصدر المساهم: | DEAKIN UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.ocn945709486 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |