رسالة جامعية
Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity
| العنوان: | Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity |
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| المؤلفون: | 加藤, 琢磨, Kato, Takuma |
| سنة النشر: | 2014 |
| ملاحظة حول المحتويات: | Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity |
| نوع الوثيقة: | 博士論文 |
| وصف الملف: | |
| اللغة: | English |
| DOI: | 10.1371/journal.pone.0097749 |
| الوصول الحر: | https://ndlsearch.ndl.go.jp/books/R100000039-I10224393Test |
| Degree: | 博士（医学） -- 香川大学 |
| ملاحظات: | 収集根拠 : 博士論文（自動収集） コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文 AbstractThe lamellipodium, an essential structure for cell migration, plays an important role in the invasion and metastasis of cancer cells. Although Rac1 recognized as a key player in the formation of lamellipodia, the molecular mechanisms underlying lamellipodial motility are not fully understood. Optogenetic technology enabled us to spatiotemporally control the activity of photoactivatable Rac1 (PA-Rac1) in living cells. Using this system, we revealed the role of phosphatidylinositol 3-kinase (PI3K) in Rac1-dependent lamellipodial motility in PC-3 prostate cancer cells. Through local blue laser irradiation of PA-Rac1-expressing cells, lamellipodial motility was reversibly induced. First, outward extension of a lamellipodium parallel to the substratum was observed. The extended lamellipodium then showed ruffling activity at the periphery. Notably, PI(3,4,5)P3 and WAVE2 were localized in the extending lamellipodium in a PI3K-dependent manner. We confirmed that the inhibition of PI3K activity greatly suppressed lamellipodial extension, while the ruffling activity was less affected. These results suggest that Rac1-induced lamellipodial motility consists of two distinct activities, PI3K-dependent outward extension and PI3K-independent ruffling. |
| رقم الانضمام: | edsdlc.oai:ndlsearch.ndl.go.jp:R100000039.I10224393 |
| قاعدة البيانات: | National Diet Library Digital Collections - 国立国会図書館デジタルコレクション |
| DOI: | 10.1371/journal.pone.0097749 |
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