المؤلفون: Zhang W, Song Q, Bi X, Cui W, Fang C, Gao J, Li J, Wang X, Qu K, Qin X, An X, Zhang C, Zhang X, Yan F, Wu G

المصدر: International Journal of Nanomedicine, Vol Volume 19, Pp 4907-4921 (2024)

مصطلحات موضوعية: pueraria lobata root-derived exosome-like nanovesicles, acute alcoholism, loss of the righting reflex, ethanol metabolism, ferroptosis., Medicine (General), R5-920

الوصف: Wenjin Zhang,1 Qiang Song,1 Xiaofei Bi,1 Wei Cui,1 Chengmei Fang,1 Jianya Gao,1 Jinan Li,1 Xiang Wang,1 Kai Qu,1 Xian Qin,1 Xuan An,2,3 Cheng Zhang,1,3 Xianxiang Zhang,1,3 Fang Yan,4 Guicheng Wu1– 3 1Chongqing Municipality Clinical Research Center for Endocrinology and Metabolic Diseases, Chongqing University Three Gorges Hospital, Chongqing, People’s Republic of China; 2Department of Hepatology, Chongqing University Three Gorges Hospital, Chongqing, People’s Republic of China; 3School of Medicine, Chongqing University, Chongqing, People’s Republic of China; 4Geriatric Diseases Institute of Chengdu, Department of Geriatrics, Chengdu Fifth People’s Hospital, Chengdu, People’s Republic of ChinaCorrespondence: Guicheng Wu, Chongqing University Three Gorges Hospital, Xincheng Road 165#, Wanzhou District, Chongqing, 404000, People’s Republic of China, Tel +(86) 58103079, Fax +86 23 581045783, Email wuguicheng@cqu.edu.cn Fang Yan, Geriatric Diseases Institute of Chengdu, Department of Geriatrics, Chengdu Fifth People’s Hospital, Mashi Street 33#, Wenjiang District, Chengdu, 611130, People’s Republic of China, Tel/Fax +86 28 82661127, Email fangyan@cdutcm.edu.cnPurpose: Pueraria lobata (P. lobata), a dual-purpose food and medicine, displays limited efficacy in alcohol detoxification and liver protection, with previous research primarily focused on puerarin in its dried roots. In this study, we investigated the potential effects and mechanisms of fresh P. lobata root-derived exosome-like nanovesicles (P-ELNs) for mitigating alcoholic intoxication, promoting alcohol metabolism effects and protecting the liver in C57BL/6J mice.Methods: We isolated P-ELNs from fresh P. lobata root using differential centrifugation and characterized them via transmission electron microscopy, nanoscale particle sizing, ζ potential analysis, and biochemical assays. In Acute Alcoholism (AAI) mice pre-treated with P-ELNs, we evaluated their effects on the timing and duration of the loss of the righting reflex (LORR), liver alcohol metabolism enzymes activity, liver and serum alcohol content, and ferroptosis-related markers.Results: P-ELNs, enriched in proteins, lipids, and small RNAs, exhibited an ideal size (150.7 ± 82.8 nm) and negative surface charge (− 31 mV). Pre-treatment with 10 mg/(kg.bw) P-ELNs in both male and female mice significantly prolonged ebriety time, shortened sobriety time, enhanced acetaldehyde dehydrogenase (ALDH) activity while concurrently inhibited alcohol dehydrogenase (ADH) activity, and reduced alcohol content in the liver and serum. Notably, P-ELNs demonstrated more efficacy compared to P-ELNs supernatant fluid (abundant puerarin content), suggesting alternative active components beyond puerarin. Additionally, P-ELNs prevented ferroptosis by inhibiting the reduction of glutathione peroxidase 4 (GPX4) and reduced glutathione (GSH), and suppressing acyl-CoA synthetase long-chain family member 4 (ACSL4) elevation, thereby mitigating pathological liver lipid accumulation.Conclusion: P-ELNs exhibit distinct exosomal characteristics and effectively alleviate alcoholic intoxication, improve alcohol metabolism, suppress ferroptosis, and protect the liver from alcoholic injury. Consequently, P-ELNs hold promise as a therapeutic agent for detoxification, sobriety promotion, and prevention of alcoholic liver injury.Keywords: Pueraria lobata root-derived exosome-like nanovesicles, acute alcoholism, loss of the righting reflex, ethanol metabolism, ferroptosis

وصف الملف: electronic resource

العلاقة: https://www.dovepress.com/preparation-ofTest-pueraria-lobata-root-derived-exosome-like-nanovesicles--peer-reviewed-fulltext-article-IJN; https://doaj.org/toc/1178-2013Test

الوصول الحر: https://doaj.org/article/0b6d1ce8c9674388a8b897975cb032d4Test

المؤلفون: Hyeseon Lee, Eunjeong Yang, Hyeok Ki Kwon, Bo Kyeong Kim, Taek Lee, Hah Young Yoo, Chulhwan Park

المصدر: Chemical and Biological Technologies in Agriculture, Vol 11, Iss 1, Pp 1-15 (2024)

مصطلحات موضوعية: Lipase, Immobilization, Flavonoid, Puerarin, Pueraria lobata, Acylation, Agriculture

الوصف: Abstract Background Puerarin, a flavonoid abundantly found in the roots of Pueraria lobata, exhibits antioxidant, anti-cancer, anti-inflammatory, and anti-aging properties. Despite its numerous benefits, the low bioavailability of puerarin hinders its widespread industrial application. To improve this, flavonoid acylation is required. Lipase is a biocatalyst primarily used for flavonoid acylation; immobilized lipase is preferred given the limited stability of free lipase. Although research on flavonoid acylation for structural modification is actively being conducted, there is a need to explore continuous production for enhancing the industrial application of flavonoid esters. Therefore, in this study, we aimed to produce puerarin palmitate innovatively by selecting an effective lipase, developing an immobilized lipase, characterizing the enzymatic reaction, and designing a semi-continuous reactor system. Results Lipase from Thermomyces lanuginosus (TL) was found to be the most suitable for the synthesis of puerarin palmitate; it was successfully immobilized on glutaraldehyde-activated silica gel (GASG). TL GASG showed greater solvent, thermal, and operational stability than those of commercial immobilized lipases. Furthermore, TL GASG use resulted in a puerarin palmitate conversion rate of 97.17% within 3 h. Additionally, the reusability of TL GASG for this reaction was higher than that of commercially available immobilized lipases. Upon using a semi-continuous reactor, the cumulative conversion rate of puerarin palmitate remained above 85% after more than ten substrate injections. Conclusions The results support the feasibility of the continuous production of flavonoid esters, underscoring their diverse industrial applications. A biorefinery strategy is anticipated to be proposed to utilize P. lobata extract biomass for flavonoid ester production. Moreover, optimizing continuous reactors through statistical experimental design is expected to enhance flavonoid ester production efficiency; these aspects will be explored in future research. Graphical Abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2196-5641Test

الوصول الحر: https://doaj.org/article/c28fdc5e7d2a4fbc9105eb131d52b190Test

المؤلفون: Yang Lv, Huan Li, Bing-Tao Zhai, Jing Sun, Jiang-Xue Cheng, Xiao-Fei Zhang, Dong-Yan Guo

المصدر: Frontiers in Pharmacology, Vol 15 (2024)

مصطلحات موضوعية: Pueraria montana var.var. lobata (Willd.) Maesen & S.M.Almeida ex Sanjappa & Predeep, Schisandra sphenanthera Rehder & E.H. Wilson, acute liver injury, symptomoriented network pharmacology, multi-omics, Therapeutics. Pharmacology, RM1-950

الوصف: BackgroundPueraria montana var. lobata (Willd.) Maesen & S.M.Almeida ex Sanjappa & Predeep (syn. Pueraria lobata (Willd.) Ohwi) and Schisandra sphenanthera Rehder & E.H. Wilson are traditional edible and medicinal hepatoprotective botanical drugs. Studies have shown that the combination of two botanical drugs enhanced the effects of treating acute liver injury (ALI), but the synergistic effect and its action mechanisms remain unclear. This study aimed to investigate the synergistic effect and its mechanism of the combination of Pueraria montana var. lobata (Willd.) Maesen & S.M.Almeida ex Sanjappa & Predeep (syn. Pueraria lobata (Willd.) Ohwi) (PM) and Schisandra sphenanthera Rehder & E.H. Wilson (SS) in the treatment of ALI.MethodsHigh performance liquid chromatography (HPLC) were utilized to conduct the chemical interaction analysis. Then the synergistic effects of botanical hybrid preparation of PM-SS (BHP PM-SS) against ALI were comprehensively evaluated by the CCl4 induced ALI mice model. Afterwards, symptom-oriented network pharmacology, transcriptomics and metabolomics were applied to reveal the underlying mechanism of action. Finally, the key target genes were experimentally by RT-qPCR.ResultsChemical analysis and pharmacodynamic experiments revealed that BHP PM-SS was superior to the single botanical drug, especially at 2:3 ratio, with a better dissolution rate of active ingredients and synergistic anti-ALI effect. Integrated symptom-oriented network pharmacology combined with transcriptomics and metabolomics analyses showed that the active ingredients of BHP PM-SS could regulate Glutathione metabolism, Pyrimidine metabolism, Arginine biosynthesis and Amino acid sugar and nucleotide sugar metabolism, by acting on the targets of AKT1, TNF, EGFR, JUN, HSP90AA1 and STAT3, which could be responsible for the PI3K-AKT signaling pathway, MAPK signaling pathway and Pathway in cancer to against ALI.ConclusionOur study has provided compelling evidence for the synergistic effect and its mechanism of the combination of BHP PM-SS, and has contributed to the development and utilization of BHP PM-SS dietary supplements.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphar.2024.1412816/fullTest; https://doaj.org/toc/1663-9812Test

الوصول الحر: https://doaj.org/article/0c9e08e7fef24e6db4119c1202398b44Test

المؤلفون: Hong-Bo Yang, Jie-Yu Song, Chan Xu, Jin Li, Chan Zhang, Sun Xie, Chun-li Teng

المصدر: Biomedicine & Pharmacotherapy, Vol 175, Iss , Pp 116780- (2024)

مصطلحات موضوعية: Pueraria oral lobata, Type 2- diabetes mellitus, Hypoglycemic effect, Metabolomics, Therapeutics. Pharmacology, RM1-950

الوصف: Pueraria lobata, commonly known as kudzu, is a medicinal and food plant widely used in the food, health food, and pharmaceutical industries. It has clinical pharmacological effects, including hypoglycemic, antiinflammatory, and antioxidant effects. However, its mechanism of hypoglycemic effect on type 2 diabetes mellitus (T2DM) has not yet been elucidated. In this study, we prepared a Pueraria lobata oral liquid (POL) and conducted a comparative study in a T2DM rat model to evaluate the hypoglycemic effect of different doses of Pueraria lobata oral liquid. Our objective was to investigate the hypoglycemic effect of Puerarin on T2DM rats and understand its mechanism from the perspective of metabolomics. In this study, we assessed the hypoglycemic effect of POL through measurements of FBG, fasting glucose tolerance test, plasma lipids, and liver injury levels. Furthermore, we examined the mechanism of action of POL using hepatic metabolomics. The study's findings demonstrated that POL intervention led to improvements in weight loss, blood glucose, insulin, and lipid levels in T2DM rats, while also providing a protective effect on the liver. Finally, POL significantly affected the types and amounts of hepatic metabolites enriched in metabolic pathways, providing an important basis for revealing the molecular mechanism of Pueraria lobata intervention in T2DM rats. These findings indicate that POL may regulate insulin levels, reduce liver damage, and improve metabolic uptake in the liver. This provides direction for new applications and research on Pueraria lobata to prevent or improve T2DM.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0753332224006644Test; https://doaj.org/toc/0753-3322Test

الوصول الحر: https://doaj.org/article/290fb8d646884c81be648e5df9759049Test

المؤلفون: Sheng CAO, Chongjian MA, Xiaohong SHANG, Fangfang WU, Pingli SHI, Huixian CHEN, Zhien TAN, Huabing YAN, Zhengdan WU, Ying WANG, Wei HAN, Baiyin YU

المصدر: Guangxi Zhiwu, Vol 43, Iss 12, Pp 2319-2327 (2023)

مصطلحات موضوعية: pueraria lobata var. thomsonii, nutrients, isoflavone components, accumulation rule, correlation, Botany, QK1-989

الوصف: In order to determine the optimal harvesting period and the application value of each part of Pueraria lobata var. thomsonii and to explore the correlation between the main nutrients and the isoflavones, P. lobata var. thomsonii of Tengxian, Guangxi was used as the material, the contents of isoflavones in different parts, the contents of main nutrients and isoflavones in different growth periods were determined by test box method, enzyme gravimetric method, Soxhlet extraction method, high performance liquid chromatography and other physiological and biochemical techniques, and the relationship between the accumulation of various nutrients and isoflavones was analyzed to clarify the accumulation rule of main nutrients and isoflavonoids and their relationships in P. lobata var. thomsonii. The results were as follows: (1)At the mature stage, the content of genistein in different parts of P. lobata var. thomsonii was not significantly different, but the content of isoflavoues aglycone in leaf was significantly lower than that in middle vine, and the contents of total isoflavones, puerarin and daidzein in root apex and vine were significantly higher than those in leaf and tuberon, and the contents of puerarin in root apex and vine were more than 1.00%. (2)The accumulation of starch, polysaccharide, crude protein, and soluble protein reached the maximum in November and December, the accumulation of insoluble dietary fiber reached the minimum in December, the accumulation of soluble dietary fiber was the maximum in August and December, and the accumulation of total isoflavones and puerarin reached the maximum in August and September. (3)The correlation analysis results showed that the accumulation of total isoflavones and puerarin was negatively correlated with the accumulation of starch and polysaccharide; the accumulation of total isoflavones and puerarin was positively correlated with the accumulation of insoluble dietary fiber. Therefore, it can be concluded that the root apex and vine of pueraria powder contain rich total isoflavones, puerarin, daidzein, which has good value for medicinal development; the best harvest time for medicinal using are August and September, and the best harvest time for edible using are November and December; the accumulation of total isoflavones and puerarin is related to starch, polysaccharide and insoluble dietary fiber. This study clarify the accumulation rule and correlation of the main nutrients and total isoflavones in P. lobata var. thomsonii, and provides a reference for the comprehensive development and utilization of P. lobata var. thomsonii and the determination of the harvest time.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1000-3142Test

الوصول الحر: https://doaj.org/article/fb863f3f36954016a6da6cd845377177Test

المؤلفون: Alex Stéphane Affian EMANFO, Kouamelan Jacques TCHINOU, Yatanan casimir BLE

المصدر: Revue Marocaine des Sciences Agronomiques et Vétérinaires, Vol 12, Iss 1 (2024)

مصطلحات موضوعية: fourrage, Panicum, Pueraria, caprin nain, paramètres zootechniques, General Works

الوصف: L’élevage des caprins nains en Afrique subsaharienne est très peu modernisé. Plusieurs facteurs zootechniques de production ne sont pas maîtrisés par les éleveurs. Ainsi, l’objectif de notre étude était de déterminer quelques paramètres zootechniques des caprins nains nourris à base de fourrage. Ainsi, 30 caprins nains ont été nourris avec deux espèces de fourrages: Panicum maximum et Pueraria phaseoloides. Chaque espèce fourragère a été présentée aux animaux sous trois formes: (1) fourrage non découpé; (2) fourrage découpé en brins de 10 cm; et (3) fourrage découpé en brins de 3 cm. Les résultats ont montré que les quantités moyennes journalières de fourrage frais ingérées (g) ont varié de 1649 g à 1839 g par jour et par caprin. De plus, le fourrage présenté en brins de 3 cm a été significativement plus consommé. Par ailleurs, les caprins nains ont consommé une plus grande quantité de P. phaseoloides. Ces résultats permettrons aux éleveurs d’affiner leur prévisions fourragères et d’éviter le gaspillage du fourrage par les caprins. Mots clés: fourrage, Panicum, Pueraria, caprin nain, paramètres zootechniques

وصف الملف: electronic resource

العلاقة: https://agrimaroc.org/index.php/Actes_IAVH2/article/view/1476Test; https://doaj.org/toc/2028-991XTest; https://doaj.org/toc/2550-4401Test

الوصول الحر: https://doaj.org/article/29506bccea76486292c095bd769d1d3fTest

المؤلفون: I. Benu, I. G. N. Jelantik, M. L. Mullik, G. E. M. Malelak, G. Oematan, M. M. Laut

المصدر: Tropical Animal Science Journal, Vol 47, Iss 1 (2024)

مصطلحات موضوعية: blood profiles, feed intake, kacang goats, Pueraria phaseoloides, supplementation, Animal culture, SF1-1100

الوصف: This study was conducted to investigate the effects of supplementing Pueraria phaseoloides on feed intake, nutrient digestibility, rumen fermentation, and blood profiles in male Kacang goats fed Kume grass hay as a basal diet. Twelve male Kacang goats aged between 6 and 8 months with a mean body weight of 13.63 ± 1.40 kg were randomly assigned to four dietary treatments (three goats per treatment) in a 3x4 incomplete Latin square design. The treatments consist of a control group (P0) receiving grass hay and concentrate and three groups supplemented with 10% (P10), 20% (P20), and 30% (P30) P. phaseoloides of protein requirement on a dry matter basis. The data obtained in this study were statistically analyzed using the GLM procedure following ANOVA in SPSS Statistics for Windows, version 22. The results indicated that P. phaseoloides significantly (p

وصف الملف: electronic resource

العلاقة: https://journal.ipb.ac.id/index.php/tasj/article/view/49135Test; https://doaj.org/toc/2615-787XTest; https://doaj.org/toc/2615-790XTest

الوصول الحر: https://doaj.org/article/f691a0119241435db75b2566f1fc5f48Test

المؤلفون: Yong Zhang, Haipeng Liu, Tianci Lv, Mengqian Xiao, Guihua Gao

المصدر: Molecules, Vol 29, Iss 12, p 2731 (2024)

مصطلحات موضوعية: PTP1B, inhibitory activity, Pueraria lobata, spectrum–effect relationship, Organic chemistry, QD241-441

الوصف: Pueraria lobata (P. lobata), a traditional anti-diabetic medicine mainly composed of flavonoids and isoflavones, has a long history in diabetes treatment in China. However, the anti-diabetic active component is still unclear. Recently, protein tyrosine phosphatase 1B (PTP1B) has been a hot therapeutic target by negatively regulating insulin signaling pathways. In this study, the spectrum–effect relationship analysis method was first used to identify the active components of P. lobata that inhibit PTP1B. The fingerprints of 12 batches of samples were established using high-performance liquid chromatography (HPLC), and sixty common peaks were identified. Meanwhile, twelve components were identified by a comparison with the standards. The inhibition of PTP1B activity was studied in vitro by using the p-nitrophenol method, and the partial least squares discriminant analysis, grey relational analysis, bivariate correlation analysis, and cluster analysis were used to analyze the bioactive compounds in P. lobata. Peaks 6, 9 (glycitin), 11 (genistin), 12 (4′-methoxypuerarin), 25, 34, 35, 36, 53, and 59 were considered as potentially active substances that inhibit PTP1B. The in vitro PTP1B inhibitory activity was confirmed by glycitin, genistin, and 4′-methoxypuerarin. The IC50s of the three compounds were 10.56 ± 0.42 μg/mL, 16.46 ± 0.29 μg/mL, and 9.336 ± 0.56 μg/mL, respectively, indicating the obvious PTP1B inhibitory activity. In brief, we established an effective method to identify PTP1B enzyme inhibitors in P. lobata, which is helpful in clarifying the material basis of P. lobata on diabetes. Additionally, it is evident that the spectrum–effect relationship method serves as an efficient approach for identifying active compounds, and this study can also serve as a reference for screening bioactive constituents in traditional Chinese medicine.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1420-3049/29/12/2731Test; https://doaj.org/toc/1420-3049Test

الوصول الحر: https://doaj.org/article/81534b06c1f946f785f2909a66288054Test

المؤلفون: Lijun Guo, Guoren Lao, Longfei He, Dong Xiao, Jie Zhan, Aiqin Wang

المصدر: International Journal of Molecular Sciences, Vol 25, Iss 11, p 5656 (2024)

مصطلحات موضوعية: Pueraria montana, mitochondrial genome, comparative analysis, RNA editing, synteny analysis, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Pueraria montana is a species with important medicinal value and a complex genetic background. In this study, we sequenced and assembled the mitochondrial (mt) genomes of two varieties of P. montana. The mt genome lengths of P. montana var. thomsonii and P. montana var. montana were 457,390 bp and 456,731 bp, respectively. Both P. montana mitogenomes showed a multi-branched structure consisting of two circular molecules, with 56 genes annotated, comprising 33 protein-coding genes, 18 tRNA genes (trnC-GCA and trnM-CAU are multi-copy genes), and 3 rRNA genes. Then, 207 pairs of long repeats and 96 simple sequence repeats (SSRs) were detected in the mt genomes of P. montana, and 484 potential RNA-editing sites were found across the 33 mitochondrial protein-coding genes of each variety. Additionally, a syntenic sequence analysis showed a high collinearity between the two mt genomes. This work is the first to analyze the mt genomes of P. montana. It can provide information that can be used to analyze the structure of mt genomes of higher plants and provide a foundation for future comparative genomic studies and evolutionary biology research in related species.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/25/11/5656Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/c0819c671d1348b2a1493413550fc2d4Test

المؤلفون: Anila Paul, Athira Ajayan, Abraham Joseph

المصدر: Next Materials, Vol 2, Iss , Pp 100130- (2024)

مصطلحات موضوعية: Pueraria phaseoloides, Polarization, EIS, Surface coating, Technology

الوصف: Pueraria phaseoloides (PP), commonly known as tropical kudzu or puero, stands as an invasive leguminous vine native to Southeast Asia; yet recent studies unveil its promising potential in myriad applications beyond its weed-like reputation. Pueraria phaseoloides seed was extracted into powder form (PPS) was followed by a comprehensive characterization employing CHNS-O, UV-Visible, FTIR, SS NMR, and TGA analysis, while surface analysis via XPS and SEM unveiled the presence of polysaccharides and proteins, encompassing S-containing protein amino acids like cysteine. The corrosion studies of epoxy coatings formulated with PPS (PPE) in acidic and saline media tagged PPS as a laudable bio-filler. Pursuant to the gravimetric analysis, the optimum concentration for superior corrosion inhibition is found to be 0.3 wt% PPE. Tafel plots confirm the viable anticorrosive characteristics of 0.3 wt% PPE coating (97.62 % and 98.08 % in acidic and saline media respectively), which are validated by electrochemical impedance spectroscopy (98.75 % and 95.00 % in acidic and saline media respectively) employing the L(R(C(R(Q(R(LR)(CR))))) equivalent circuit model, with constant protection efficiency values across all techniques. Assessing the corrosion studies of PPE composite coating, turned the table of the invasive weed into a powerful corrosion deterrent.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2949822824000273Test; https://doaj.org/toc/2949-8228Test

الوصول الحر: https://doaj.org/article/a72c38f9879846bd96307d2d40be3cd0Test