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Moderate Peep After Tracheal Lipopolysaccharide Instillation Prevents Inflammation and Modifies the Pattern of Brain Neuronal Activation

التفاصيل البيبلوغرافية
العنوان: Moderate Peep After Tracheal Lipopolysaccharide Instillation Prevents Inflammation and Modifies the Pattern of Brain Neuronal Activation
المؤلفون: Quilez, María Elisa, Rodríguez-González, Raquel, Turon, Marc, Fernandez-Gonzalo, Sol, Villar, Jesús, Kacmarek, Robert M., Gómez, Ma Nieves, Oliva, Joan Carles, Blanch, Lluís, López-Aguilar, Josefina
المصدر: Quilez, María Elisa, Raquel Rodríguez-González, Marc Turon, Sol Fernandez-Gonzalo, Jesús Villar, Robert M. Kacmarek, Ma Nieves Gómez, Joan Carles Oliva, Lluís Blanch, and Josefina López-Aguilar. 2015. “Moderate Peep After Tracheal Lipopolysaccharide Instillation Prevents Inflammation and Modifies the Pattern of Brain Neuronal Activation.” Shock (Augusta, Ga.) 44 (6): 601-608. doi:10.1097/SHK.0000000000000469. http://dx.doi.org/10.1097/SHK.0000000000000469Test.
بيانات النشر: Lippincott Williams & Wilkins, 2015.
سنة النشر: 2015
المجموعة: HMS Scholarly Articles
مصطلحات موضوعية: Experimental, inflammation, mechanical ventilation, neuronal activation, PEEP, ALI, Acute lung injury, ARDS, Acute respiratory distress syndrome, BAS, Basal, CeA, Central amygdala, IL, Interleukin, LPS, lipopolysaccharide, MAP, Mean arterial pressure, MCP, Monocyte chemoattractant protein, MV, Mechanical ventilation, NTS, Nucleus tractus solitarius, Positive end-expiratory pressure, PVN, Paraventricular nucleus, RS, Retrosplenial cortex, SEM, Standard error of the mean, SON, Supraoptic nucleus, TNF, Tumor necrosis factor, VILI, Ventilator-induced lung injury, VT, Tidal volume
الوصف: Background: Ventilatory strategy and specifically positive end-expiratory pressure (PEEP) can modulate the inflammatory response and pulmonary-to-systemic translocation of lipopolysaccharide (LPS). Both inflammation and ventilatory pattern may modify brain activation, possibly worsening the patient's outcome and resulting in cognitive sequelae. Methods: We prospectively studied Sprague–Dawley rats randomly assigned to undergo 3 h mechanical ventilation with 7 mL/kg tidal ventilation and either 2 cmH2O or 7 cmH2O PEEP after intratracheal instillation of LPS or saline. Healthy nonventilated rats served as baseline. We analyzed lung mechanics, gas exchange, lung and plasma cytokine levels, lung apoptotic cells, and lung neutrophil infiltration. To evaluate brain neuronal activation, we counted c-Fos immunopositive cells in the retrosplenial cortex (RS), thalamus, supraoptic nucleus (SON), nucleus of the solitary tract (NTS), paraventricular nucleus (PVN), and central amygdala (CeA). Results: LPS increased lung neutrophilic infiltration, lung and systemic MCP-1 levels, and neuronal activation in the CeA and NTS. LPS-instilled rats receiving 7 cmH2O PEEP had less lung and systemic inflammation and more c-Fos-immunopositive cells in the RS, SON, and thalamus than those receiving 2 cmH2O PEEP. Applying 7 cmH2O PEEP increased neuronal activation in the CeA and NTS in saline-instilled rats, but not in LPS-instilled rats. Conclusions: Moderate PEEP prevented lung and systemic inflammation secondary to intratracheal LPS instillation. PEEP also modified the neuronal activation pattern in the RS, SON, and thalamus. The relevance of these differential brain c-Fos expression patterns in neurocognitive outcomes should be explored.
نوع الوثيقة: Journal Article
اللغة: English
تدمد: 1073-2322
العلاقة: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851224/pdfTest/; Shock (Augusta, Ga.)
DOI: 10.1097/SHK.0000000000000469
الوصول الحر: http://nrs.harvard.edu/urn-3:HUL.InstRepos:27320343Test
حقوق: open
URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAATest
رقم الانضمام: edshld.1.27320343
قاعدة البيانات: Digital Access to Scholarship at Harvard (DASH)
الوصف
تدمد:10732322
DOI:10.1097/SHK.0000000000000469