دورية أكاديمية
Rapid disorganization of mechanically interacting systems of mammary acini
| العنوان: | Rapid disorganization of mechanically interacting systems of mammary acini |
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| المؤلفون: | Shi, Qianyun, Ghosh, R. P., Engelke, H., Rycroft, Christopher, Cassereau, L., Sethian, J. A., Weaver, V. M., Liphardt, J. T. |
| المصدر: | Shi, Q., R. P. Ghosh, H. Engelke, C. H. Rycroft, L. Cassereau, J. A. Sethian, V. M. Weaver, and J. T. Liphardt. 2013. “Rapid Disorganization of Mechanically Interacting Systems of Mammary Acini.” Proceedings of the National Academy of Sciences 111 (2) (December 30): 658–663. doi:10.1073/pnas.1311312110. |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | FAS Scholarly Articles |
| الوصف: | Cells and multicellular structures can mechanically align and concentrate fibers in their ECM environment and can sense and respond to mechanical cues by differentiating, branching, or disorganizing. Here we show that mammary acini with compromised structural integrity can interconnect by forming long collagen lines. These collagen lines then coordinate and accelerate transition to an invasive phenotype. Interacting acini begin to disorganize within 12.5 ± 4.7 h in a spatially coordinated manner, whereas acini that do not interact mechanically with other acini disorganize more slowly (in 21.8 ± 4.1 h) and to a lesser extent (P < 0.0001). When the directed mechanical connections between acini were cut with a laser, the acini reverted to a slowly disorganizing phenotype. When acini were fully mechanically isolated from other acini and also from the bulk gel by box-cuts with a side length <900 μm, transition to an invasive phenotype was blocked in 20 of 20 experiments, regardless of waiting time. Thus, pairs or groups of mammary acini can interact mechanically over long distances through the collagen matrix, and these directed mechanical interactions facilitate transition to an invasive phenotype. Engineering and Applied Sciences |
| نوع الوثيقة: | Journal Article |
| اللغة: | English |
| تدمد: | 0027-8424 |
| العلاقة: | Proceedings of the National Academy of Sciences |
| DOI: | 10.1073/pnas.1311312110 |
| الوصول الحر: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:32193499Test |
| حقوق: | open URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAATest |
| رقم الانضمام: | edshld.1.32193499 |
| قاعدة البيانات: | Digital Access to Scholarship at Harvard (DASH) |
Full Text Finder | تدمد: | 00278424 |
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| DOI: | 10.1073/pnas.1311312110 |