المؤلفون: Yang, Dan-Dan, Li, Shu-Yi, Xu, Xiao-Wei, Li, Qing-Yao, He, Jia-Yuan, Zhou, Lian-Di, Zhang, Qi-Hui

المصدر: American Journal of Chinese Medicine; 2024, Vol. 52 Issue 4, p1137-1154, 18p

مصطلحات موضوعية: FOLIAR diagnosis, POLYMERS, ADSORPTION (Chemistry), HIGH performance liquid chromatography, COMPUTER simulation, PHARMACEUTICAL technology, RESEARCH funding, FLAVONOIDS, PHARMACEUTICAL chemistry, DYNAMICS, ELECTRON microscopy, HERBAL medicine, QUERCETIN, FLAVONOLS, MOLECULAR structure, ANTIOXIDANTS, SCANNING electron microscopy, SPECTRUM analysis, ANALYTICAL chemistry

مستخلص: Flavonol and flavonoid compounds are important natural compounds with various biomedical activities. Therefore, it is of great significance to develop a strategy for the specific extraction of flavonol and flavonoid compounds. Quercetin is a well-studied flavonoid possessing many health benefits. This compound is a versatile antioxidant known to possess protective abilities against body tissue injury induced by pathological situations and various drug toxicities. Although quercetin is widely distributed in many plants, its content generally is not very high. Therefore, the specific extraction of quercetin as well as other flavonol and flavonoid compounds has profound significance. In this work, the quercetin molecularly imprinting polymer (QMIP) was successfully prepared, in which a typical flavonol quercetin was selected as the template molecule. QMIP was synthesized by performing the surface molecular imprinting technology on the surface of NH2-MIL-101(Fe). Our study results showed that QMIP exhibited quick binding kinetic behavior, a high adsorption capacity (57.04 mg/g), and the specific recognition ability toward quercetin compared with structurally distinct compounds (selective factor > 2). The specific adsorption ability of quercetin by QMIP was further explained using computation simulation that molecules with non-planar 3D conformations hardly entered the molecularly imprinted cavities on QMIP. Finally, QMIP was successfully used for the specific extraction of quercetin and five other flavonol and flavonoid compounds in the crude extracts from Sapium sebiferum. This study proposes a new strategy to synthesize the molecularly imprinted polymer based on a single template for enriching and loading a certain class of active ingredients with similar core structures from variable botanicals. [ABSTRACT FROM AUTHOR]

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المؤلفون: Lei Wang, Xiao Lei, Qiao-Liang Zhang, Guang-Qing Yao, Bo Sui, Xiao-Jun Chen, Ming-Wei Wang, Zhen-Yu Zhou, Pan-Rong Wang, Xiao-Dong Peng

المصدر: Petroleum Science (KeAi Communications Co.); Apr2024, Vol. 21 Issue 2, p1044-1061, 18p

مصطلحات موضوعية: SEEPAGE, SANDSTONE, TWO-phase flow, DRILL core analysis, FLOODS, GRAIN size

مستخلص: To investigate the relationship between grain sizes, seepage capacity, and oil-displacement efficiency in the Liushagang Formation of the Beibuwan Basin, this study identifies the multistage pore-throat structure as a crucial factor through a comparison of oil displacement in microscopic pore-throat experiments. The two-phase flow evaluation method based on the LieHorne model is utilized to effectively characterize and quantify the seepage characteristics of different reservoirs, closely relating them to the distribution of microscopic pores and throats. It is observed that conglomerate sandstones at different stages exhibit significant heterogeneity and noticeable differences in seepage capacity, highlighting the crucial role played by certain large pore throats in determining seepage capacity and oil displacement efficiency. Furthermore, it was found that the displacement effects of conglomeratic sandstones with strong heterogeneity were inferior to those of conventional homogeneous sandstone, as evidenced by multiple displacement experiments conducted on core samples with varying granularities and flooding systems. Subsequently, core-based experiments on associated gas flooding after water flooding were conducted to address the challenge of achieving satisfactory results in a single displacement mode for reservoirs with significant heterogeneity. The results indicate that the oil recovery rates for associated gas flooding after water flooding increased by 7.3%e16.4% compared with water flooding alone at a gas eoil ratio of approximately 7000 m³/m³. Therefore, considering the advantages of gas flooding in terms of seepage capacity, oil exchange ratio, and the potential for two-phase production, gas flooding is recommended as an energy supplement mode for homogeneous reservoirs in the presence of sufficient gas source and appropriate tectonic angle. On the other hand, associated gas flooding after water flooding is suggested to achieve a more favorable development effect compared to a single mode of energy supplementation for strongly heterogeneous sandstone reservoirs. [ABSTRACT FROM AUTHOR]

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المؤلفون: Na Hu, Ying Wu, Qing Yao, Shixiang Huang, Wen Li, Zhenhua Yao, Chunfeng Ye

المصدر: Frontiers in Pediatrics; 2024, p1-11, 11p

المؤلفون: Yi Zang, Xianjiao Zhang, Zhe Wang, Qingyi Tong, Yang Zhou, Qing Yao, Honghui Zhu

المصدر: Frontiers in Microbiology; 2024, p1-11, 11p

مصطلحات موضوعية: METABOLITES, BIOLOGICAL assay, GRAM-negative bacteria, DENSITY functional theory, MYXOBACTERALES

مصطلحات جغرافية: CHINA

مستخلص: Myxobacteria are special bacteria with wide adaptability, which are rich sources of structurally diverse natural products with intriguing biological properties. Here, a gram-negative myxobacterium strain s54d21T was isolated from the sediment of a wetland park in China using the Escherichia coli baiting method. Based on 16S rRNA gene sequence and genomic data, the strain was demonstrated to be a novel species of a rare genus Hyalangium, designated Hyalangium ruber sp. nov (type strain s54d21T = GDMCC 1.1945T = JCM 39263T). The subsequent chemical investigation of the strain s54d21T led to the isolation of three rare 3,5,6-trisubstituted 2(1H)-pyrazinones, namely, hyalanones A-C (1-3), together with a known macrolactin A (4). Those new structures and their absolute configurations were unambiguously assigned by extensive analyses of spectroscopic data and density functional theory (DFT) calculations. In biological assays, compound 4 exhibited moderate cytotoxic activities against human cell lines RKO, A549, and NCM460 with IC50 values ranging from 27.21 to 32.14 µM. [ABSTRACT FROM AUTHOR]

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المؤلفون: Xilong Yin, Wei Zhang, Zengwei Feng, Guangda Feng, Honghui Zhu, Qing Yao

المصدر: Horticulture Plant Journal; Mar2024, Vol. 10 Issue 2, p425-436, 12p

مصطلحات موضوعية: CITRUS, VESICULAR-arbuscular mycorrhizas, EFFECT of drought on plants, CELLULAR aging, ABIOTIC stress, PLANT growth

مستخلص: Citrus is the typical mycorrhizal fruit tree species establishing symbiosis with arbuscular mycorrhizal (AM) fungi. However, arbuscule development and senescence in colonized citrus roots, especially in response to drought stress, remain unclear, which is mainly due to the difficulty in clearing and staining lignified roots with the conventional method. Here, we improved the observation of colonized roots of citrus plants with the sectioning method, which enabled the clear observation of AM fungal structures. Furthermore, we investigated the effects of one week of drought stress on arbuscule development and senescence with the sectioning method. Microscopy observations indicated that drought stress significantly decreased mycorrhizal colonization (F% and M%) although it did not affect plant growth performance. Fluorescence probes (WGA 488 and/or Nile red) revealed that drought stress inhibited arbuscule development by increasing the percentage of arbuscules at the early stage and decreasing the percentages of arbuscules at the midterm and mature stages. Meanwhile, drought stress accelerated arbuscule senescence, which was characterized by the increased accumulation of neutral lipids. Overall, the sectioning method developed in this study enables the in-depth investigation of arbuscule status, and drought stress can inhibit arbuscule development but accelerate arbuscule senescence in the colonized roots of citrus plants. This study paves the way to elaborately dissecting the arbuscule dynamics in the roots of fruit tree species in response to diverse abiotic stresses. [ABSTRACT FROM AUTHOR]

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المؤلفون: Chubing Long, Dong Lin, Lieliang Zhang, Yue Lin, Qing Yao, Guangyong Zhang, Longshan Li, Hailin Liu, Jun Ying, Xifeng Wang, Fuzhou Hua

المصدر: Frontiers in Endocrinology; 2024, p1-10, 10p

مصطلحات موضوعية: LDL cholesterol, DELIRIUM, GENOME-wide association studies

مستخلص: Background: Delirium significantly contributes to both mortality and morbidity among hospitalized older adults. Furthermore, delirium leads to escalated healthcare expenditures, extended hospital stays, and enduring cognitive deterioration, all of which are acknowledged detrimental outcomes. Nonetheless, the current strategies for predicting and managing delirium remain constrained. Our aim was to employ Mendelian randomization (MR) to investigate the potential causal relationship between metabolites and delirium, as well as to identify potential therapeutic targets. Methods: We identified 129 distinct blood metabolites from three genome-wide association studies (GWASs) conducted on the metabolome, involving a total of 147,827 participants of European descent. Genetic information pertaining to delirium was sourced from the ninth iteration of the Finngen Biobank, encompassing 359,699 individuals of Finnish ancestry. We conducted MR analyses to evaluate the connections between blood metabolites and delirium. Additionally, we extended our analysis to encompass the entire phenome using MR, aiming to uncover potential on-target consequences resulting from metabolite interventions. Results: In our investigation, we discovered three metabolites serving as causal mediators in the context of delirium: clinical low density lipoprotein cholesterol (LDL-C) (odds ratio [OR]: 1.47, 95% confidence interval [CI]: 1.25-1.73, p = 3.92 x 10^-6), sphingomyelin (OR: 1.47, 95% CI: 1.25-1.74, p = 5.97 x 10^-6), and X-11593-O-methylascorbate (OR: 0.21, 95% CI: 0.10-0.43, p = 1.86 x 10^-5). Furthermore, utilizing phenome-wide MR analysis, we discerned that clinical LDL-C, sphingomyelin, and O-methylascorbate not only mediate delirium susceptibility but also impact the risk of diverse ailments. Limitations: (1) Limited representation of the complete blood metabolome, (2) reliance on the PheCode system based on hospital diagnoses may underrepresent conditions with infrequent hospital admissions, and (3) limited to European ancestry. Conclusion: The genetic prediction of heightened O-methylascorbate levels seems to correspond to a diminished risk of delirium, in contrast to the association of elevated clinical LDL-C and sphingomyelin levels with an amplified risk. A comprehensive analysis of side-effect profiles has been undertaken to facilitate the prioritization of drug targets. Notably, Omethylascorbate emerges as a potentially auspicious target for mitigating and treating delirium, offering the advantage of lacking predicted adverse side effects. [ABSTRACT FROM AUTHOR]

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المؤلفون: Yang Zhou, Haixin Chen, Hongxia Jiang, Qing Yao, Honghui Zhu

المصدر: Frontiers in Microbiology; 2024, p1-11, 11p

مصطلحات موضوعية: LIPASES, ESCHERICHIA coli, BIOLOGICAL pest control agents, GRAM-negative bacteria, PATHOGENIC microorganisms, SWINE farms, STAPHYLOCOCCUS aureus, METHICILLIN-resistant staphylococcus aureus

مستخلص: Bacteriolytic myxobacteria are versatile micropredators and are proposed as potential biocontrol agents against diverse bacterial and fungal pathogens. Isolation of new myxobacteria species and exploration of effective predatory products are necessary for successful biocontrol of pathogens. In this study, a myxobacterium strain CY-1 was isolated from a soil sample of a pig farm using the Escherichia coli baiting method. Based on the morphological observation, physiological test, 16S rRNA gene sequence, and genomic data, strain CY-1 was identified as a novel species of the myxobacterial genus Archangium, for which the name Archangium lipolyticum sp. nov. was proposed. Subsequent predation tests indicated that the strain efficiently lysed drug-resistant pathogens, with a higher predatory activity against E. coli 64 than Staphylococcus aureus GDMCC 1.771 (MRSA). The lysis of extracellular proteins against ester-bond-containing substrates (tributyrin, tween 80, egg-yolk, and autoclaved drug-resistant pathogens) inspired the mining of secreted predatory products with lipolytic activity. Furthermore, a lipase ArEstA was identified from the genome of CY-1, and the heterologously expressed and purified enzyme showed bacteriolytic activity against Gramnegative bacteria E. coli 64 but not against Gram-positive MRSA, possibly due to different accessibility of enzyme to lipid substrates in different preys. Our research not only provided a novel myxobacterium species and a candidate enzyme for the development of new biocontrol agents but also reported an experimental basis for further study on different mechanisms of secreted predatory products in myxobacterial killing and degrading of Gram-negative and Gram-positive preys. [ABSTRACT FROM AUTHOR]

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المؤلفون: Pan Xu, Qing-Yao Luo, Bo Dong, Bo Song

المصدر: Molecular Simulation; Jan2024, Vol. 50 Issue 2, p104-116, 13p

مصطلحات موضوعية: THERMODYNAMICS, PROPERTIES of fluids, MOLECULAR dynamics, SPEED of sound, HEAT capacity

الشركة/الكيان: NATIONAL Institute of Standards & Technology (U.S.)

مستخلص: The molecular dynamics simulation method is used to study the internal energy, pressure, isochoric heat capacity, and sound speed of helium based on the state-of-the-art ab initio potentials. The simulations cover a wide temperature and density range of (20-2000) K and (0.0005-70) mol L-1. The uncertainty of simulation data are evaluated based on the uncertainty of the potential and the uncertainty of the simulation method. At temperatures below 300 K, the quantum Feynman-Hibbs modified potential and the Wigner-Kirkwood modified potential are introduced and the results are almost the same as those by the original ab initio potential. The modified potentials can not reasonably describe the quantum effects for the helium fluid at low temperatures, which become obvious below 200 K. The two-body ab initio potential is combined with the three-body ab initio potential to evaluate the influence of multi-body interactions at high densities. When the density is lower than 45 mol L-1, the contribution of the three-body term to our simulation data is not significant. As a result, the threebody potential is omitted in our calculations to improve the overall computational efficiency. The thermodynamic property data of this work show agreement with the experimental data in the literature as well as the NIST Refprop 10.0 data at temperatures above 200 K and densities below 45 mol L-1. [ABSTRACT FROM AUTHOR]

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المؤلفون: Fei Xiao, Han-Qing Yao, Jing Qian, Jia-Li Deng, Zheng-Fen Xu, Lin Liu, Xin-Zhong Chen

المصدر: Anesthesia & Analgesia; Dec2023, Vol. 137 Issue 6, p1233-1240, 8p

المؤلفون: Kaili Xia, Zengwei Feng, Xianjiao Zhang, Yang Zhou, Honghui Zhu, Qing Yao

المصدر: Frontiers in Plant Science; 2023, p01-14, 14p

مصطلحات موضوعية: CANDIDATUS liberibacter asiaticus, POTENTIAL functions, NUCLEOTIDE sequencing, CITRUS, RANDOM forest algorithms, FRUIT trees

مستخلص: Instruction: Citrus is a globally important fruit tree whose microbiome plays a vital role in its growth, adaptability, and resistance to stress. Methods: With the high throughput sequencing of 16S rRNA genes, this study focused on analyzing the bacterial community, especially in the leaf midribs, of healthy and Huanglongbing (HLB)-infected plants. Results: We firstly identified the shared bacterial taxa in the midribs of both healthy and HLB-infected plants, and then analyzed their functions. Results showed that the shared bacterial taxa in midribs belonged to 62 genera, with approximately 1/3 of which modified in the infected samples. Furthermore, 366 metabolic pathways, 5851 proteins, and 1833 enzymes in the shared taxa were predicted. Among these, three metabolic pathways and one protein showed significant importance in HLB infection. With the random forest method, six genera were identified to be significantly important for HLB infection. Notably, four of these genera were also among the significantly different shared taxa. Further functional characterization of these four genera revealed that Pseudomonas and Erwinia likely contributed to plant defense against HLB, while Streptomyces might have implications for plant defense against HLB or the pathogenicity of Candidatus Liberibacter asiaticus (CLas). Disccusion: Overall, our study highlights that the functions of the shared taxa in leaf midribs are distinguished between healthy and HLB-infected plants, and these microbiome-based findings can contribute to the management and protection of citrus crops against CLas. [ABSTRACT FROM AUTHOR]

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