المؤلفون: Encinas, Micaela, Ferrara Muñiz, Ximena, Ayelén Sammarruco, Romina, Ruiz Menna, Victoria, Javier Garro, Carlos, Delgado, Fernando, Macías, Analía, Magnano, Gabriel, Zumárraga, Martín José, Garbaccio, Sergio Gabriel, Eirin, María Emilia

المصدر: Frontiers in Veterinary Science; 2024, p1-6, 6p

مصطلحات موضوعية: TUBERCULIN test, MYCOBACTERIUM bovis, SKIN tests, COW testing, TUBERCULOSIS in cattle

مستخلص: Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and the ...-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern of multi-organ lesions attributable to Mycobacterium bovis (the causative agent of bTB) bacteraemia. A case--control study was conducted to investigate blood PCR as an alternative tool for improving ante-mortem detection of TST false-negative bovines. Cases comprised 70 TST false-negative bovines (cases), which were serology positive, and controls included 81 TST positive bovines; all of them confirmed as infected with M. bovis. Detection of the IS6110 target through touchdown blood-PCR (IS6110 TD-PCR) was performed. The positivity of the blood-PCR was 27.2% in the control group. This performance was similar to the 15% obtained among cases (p = 0.134). Most cases identified by the IS6110 TD-PCR exhibited focalized lesions (p = 0.002). Results demonstrated that blood-PCR could detect TST false-negative cattle, even if they are negative for IGRA. Considering that cases exhibited humoral response to M. bovis, further studies conducted in a pre-serological stage could provide evidence about the real contribution of the technique in herds. [ABSTRACT FROM AUTHOR]

: Copyright of Frontiers in Veterinary Science is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Morris, Winston E., Delgado, Fernando O., Micheloud, Juan F., Garro, Carlos J., Olguin‐Perglione, Cecilia, Ebinger, Marem, Gimeno, Eduardo J., Garbaccio, Sergio G., Vilte, Daniel A.

المصدر: Veterinary Record Case Reports; Dec2023, Vol. 11 Issue 4, p1-4, 4p

مصطلحات موضوعية: ANIMAL herds, AUTOPSY, BOS, RECESSIVE genes, CALVES, HISTOCHEMISTRY, ELECTRON microscopy

مصطلحات جغرافية: BUENOS Aires (Argentina : Province), ARGENTINA

مستخلص: An outbreak of α‐mannosidosis in a bovine herd from Buenos Aires province is reported. Postmortem examination, histology, lectin histochemistry and electron microscopy were performed on five calves born with nervous signs. Hair samples from dams and calves were genetically analysed. The α‐mannosidase gene including exon 7 was amplified and sequenced. At postmortem examination, no gross changes were evident. Histologically, cytoplasmic neuronal vacuolation in the brain and cerebellum of the five calves, staining moderately to intensively, with lectin histochemistry was evident. Ultrastructurally, vacuoles containing finely granulated material in the cytoplasm of neurons, myelin degeneration and axonal swelling were seen in the brain. Genetic analysis revealed a heterozygous carrier state for 961T→C transition in the exon 7 of α‐mannosidosis gene of four cows, and a recessive homozygous state for 961T→C transition in the same genes in the calves analysed. This highlights the importance of identifying and removing animals carrying the α‐mannosidosis mutated gene. [ABSTRACT FROM AUTHOR]

: Copyright of Veterinary Record Case Reports is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Marfil, María Jimena, Huertas, Pablo Sebastián, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Martínez Vivot, Marcela, Garro, Carlos, Alonso, Bernardo, Eirin, María Emilia, Zumárraga, Martín José

المصدر: Foodborne Pathogens & Disease; Dec2018, Vol. 15 Issue 12, p758-762, 5p