التفاصيل البيبلوغرافية
| العنوان: |
Activation of a nuclear-localized SIPK in tobacco cells challenged by cryptogein, an elicitor of plant defence reactions. |
| المؤلفون: |
Jennifer Dahan, Carole Pichereaux, Michel Rossignol, Sabrina Blanc, David Wendehenne, Alain Pugin, Stéphane Bourque |
| المصدر: |
Biochemical Journal; 2009, Vol. 418 Issue 1, p191-200, 10p |
| مصطلحات موضوعية: |
PLANT defenses, PLANT cells & tissues, DEFENSE reaction (Physiology), CELLULAR signal transduction, PROTEIN kinases, PHOSPHORYLATION, MITOGEN-activated protein kinases |
| مستخلص: |
When a plant cell is challenged by a well-defined stimulus, complex signal transduction pathways are activated to promote the modulation of specific sets of genes and eventually to develop adaptive responses. In this context, protein phosphorylation plays a fundamental role through the activation of multiple protein kinase families. Although the involvement of protein kinases at the plasma membrane and cytosolic levels are now well-documented, their nuclear counterparts are still poorly investigated. In the field of plant defence reactions, no known study has yet reported the activation of a nuclear protein kinase and/or its nuclear activity in plant cells, although some protein kinases, e.g. MAPK (mitogen-activated protein kinase), are known to be translocated into the nucleus. In the present study, we investigated the ability of cryptogein, a proteinaceous elicitor of tobacco defence reactions, to induce different nuclear protein kinase activities. We found that at least four nuclear protein kinases are activated in response to cryptogein treatment in a time-dependent manner, some of them exhibiting Ca2+dependent activity. The present study focused on one 47Â kDa protein kinase with a Ca2+independent activity, closely related to the MAPK family. After purification and microsequencing, this protein kinase was formally identified as SIPK (salicyclic acid-induced protein kinase), a biotic and abiotic stress-activated MAPK of tobacco. We also showed that cytosolic activation of SIPK is not sufficient to promote a nuclear SIPK activity, the latter being correlated with cell death. In that way, the present study provides evidence of a functional nuclear MAPK activity involved in response to an elicitor treatment. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
