المؤلفون: Jiahui Wang, Rong Luo, Xia Zhao, Di Xia, Yi Liu, Tao Shen, Yuanjiao Liang

المصدر: Frontiers in Endocrinology; 2023, p1-11, 11p

مصطلحات موضوعية: GUT microbiome, GENOME-wide association studies, ANALYSIS of variance, EUBACTERIALES, ODDS ratio

مستخلص: Background: Recent studies have indicated a potential correlation between intestinal bacteria and primary ovarian insufficiency (POI). However, the causal relationship between the gut microbiota (GM) and POI remains unclear. Methods: A bidirectional two-sample Mendelian randomization (MR) study was conducted to investigate the relationship between the GM and POI. Data on the GM were based on the MiBioGen consortium's summary statistics from the most comprehensive genome-wide association study meta-analysis to date (n=13,266), and POI data were obtained from the R8 release of the FinnGen consortium, containing a total of 424 cases and 181,796 controls. A variety of analytical methods, including inverse variance weighting, maximum likelihood, MR-Egger, weighted median, and constrained maximum likelihood and model averaging and Bayesian information criterion, were utilized to explore the connection between the GM and POI. The Cochran's Q statistics were used to evaluate the heterogeneity of instrumental variables. The MR-Egger and MRpleiotropy residual sum and outlier (PRESSO) methods were used to identify the horizontal pleiotropy of instrumental variables. The MR Steiger test was used to evaluate the strength of causal relationships. A reverseMR study was performed to investigate the causal relationship between POI and the targeted GMs which were indicated to have a causal relationship with POI in the forward MR evaluation. Results: The inverse variance weighted analysis indicated that Eubacterium (hallii group) (odds ratio [OR]=0.49, 95% confidence interval [CI]: 0.26-0.9, P=0.022) and Eubacterium (ventriosum group) (OR=0.51, 95% CI: 0.27-0.97, P=0.04) had protective effects on POI, and Intestinibacter (OR=1.82, 95% CI: 1.04-3.2, P=0.037) and Terrisporobacter (OR=2.47, 95% CI: 1.14-5.36, P=0.022) had detrimental effects on POI. Results of the reverse MR analysis indicated that POI had no significant influence on the four GMs. No significant heterogeneity or horizontal pleiotropy was observed in the performanceof the instrumental variables. Conclusion: This bidirectional two-sample MR study revealed a causal link between Eubacterium (hallii group), Eubacterium (ventriosum group), Intestinibacter, and Terrisporobacter and POI. Additional clinical trials are needed to gain a clearer understanding of the beneficial or detrimental effects of the GMs on POI and their mechanisms of action. [ABSTRACT FROM AUTHOR]

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المؤلفون: Wang, Guang-Qing, Tang, Tao, Wang, Zhong-Shan, Liu, Ying-Ying, Wang, Li, Luo, Peng-Fei, Xia, Zhao-Fan

المصدر: PLoS ONE; 8/10/2016, Vol. 11 Issue 8, p1-14, 14p

مصطلحات موضوعية: SEPSIS, GENETIC overexpression, HEART function tests, NF-kappa B, CYTOKINES, HEART cells, TRANSGENIC mice

مستخلص: IκBβis an inhibitor of nuclear factor kappa B(NF-κB) and participates in the cardiac response to sepsis. However, the role of the hypo-phosphorylated form of IκBβ at Ser313, which can be detected during sepsis, is unknown. Here, we examined the effects of IκBβ with a mutation at Ser313→Ala313 on cardiac damage induced by sepsis. Transgenic (Tg) mice were generated to overexpress IκBβ, in which Ser-313 is replaced with alanine ubiquitously, in order to mimic the hypo-phosphorylated form of IκBβ. Survival analysis showed that Tg mice exhibited decreased inflammatory cytokine levels and decreased rates of mortality in comparison to wild type (WT) mice, after sepsis in a cecal-ligation and puncture model (CLP). Compared to WT septic mice, sepsis in Tg mice resulted in improved cardiac functions, lower levels of troponin I and decreased rates of cardiomyocyte apoptosis, compared to WT mice. The increased formation of autophagicvacuoles detected with electron microscopy demonstrated the enhancement of cardiac autophagy. This phenomenon was further confirmed by the differential expression of genes related to autophagy, such as LC3, Atg5, Beclin-1, and p62. The increased expression of Cathepsin L(Ctsl), a specific marker for mitochondrial stress response, may be associated with the beneficial effects of the hypo-phosphorylated form of IκBβ. Our observations suggest that the hypo-phosphorylated form of IκBβ at Ser313 is beneficial to the heart in sepsis through inhibition of apoptosisand enhancement of autophagy in mutated IκBβ transgenic mice. [ABSTRACT FROM AUTHOR]

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المؤلفون: Wang, Yu, Sun, Yu, Yang, Xiao‐Yan, Ji, Shi‐Zhao, Han, Shu, Xia, Zhao‐Fan

المصدر: International Wound Journal; Aug2013, Vol. 10 Issue 4, p473-479, 7p, 1 Diagram, 1 Chart, 3 Graphs

مصطلحات موضوعية: SKIN injuries, ANALYSIS of variance, ANIMAL experimentation, BONE marrow, BURNS & scalds, CELLS, CYTOKINES, MICE, RESEARCH funding, T-test (Statistics), WOUND healing, WOUNDS & injuries, DATA analysis software

مستخلص: Massive skin defects caused by severe burn and trauma are a clinical challenge to surgeons. Timely and effective wound closure is often hindered by the lack of skin donor site. Bone marrow-derived cells (BMDCs) have been shown to 'differentiate' into multiple tissue cells. In this study we focused on the direct manipulation of endogenous BMDCs, avoiding the immunocompatibility issues and complicated cell isolation, purification, identification and amplification procedures in vitro on wound repair. We found that mobilisation of the BMDCs into the circulation significantly increased the amount of BMDCs at the injury site which in turn accelerated healing of large open wound. We used a chimeric green fluorescent protein (GFP) mouse model to track BMDCs and to investigate their role in full-thickness skin excisional wounds. We have shown that bone marrow mobilisation by granulocyte colony stimulating factor (G-CSF) exerted multiple beneficial effects on skin repair, both by increasing the engraftment of BMDCs into the skin to differentiate into multiple skin cell types and by upregulating essential cytokine mRNAs critical to wound repair. The potential trophic effects of G-CSF on bone marrow stem cells to accelerate wound healing could have a significant clinical impact. [ABSTRACT FROM AUTHOR]

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المؤلفون: Yan Sun, Hui-Juan Xu, Yan-Xia Zhao, Ling-Zhen Wang, Li-Rong Sun, Zhi Wang, Xiu-Fang Sun

المصدر: Evidence-based Complementary & Alternative Medicine (eCAM); 2013, Vol. 2013, p1-7, 7p, 6 Graphs

مصطلحات موضوعية: CAROTENOIDS, TISSUE analysis, THERAPEUTIC use of plant extracts, AGAR, ANALYSIS of variance, ANIMAL experimentation, ANTHROPOMETRY, BIOLOGICAL assay, BIOPHYSICS, CELL cycle, CULTURES (Biology), DOSE-effect relationship in pharmacology, ELECTROPHORESIS, ENZYME-linked immunosorbent assay, LYMPHOBLASTIC leukemia, RESEARCH methodology, MICE, STAINS & staining (Microscopy), TIME, WESTERN immunoblotting, XENOGRAFTS, ACUTE myeloid leukemia, ABSORPTION, DATA analysis software, GENE expression profiling, DESCRIPTIVE statistics, CANCER cell culture, IN vitro studies, THERAPEUTICS

مستخلص: Crocin is a carotenoid of the saffron extract that exhibits antitumor activity against many human tumors. However, the effects of crocin on HL-60 cells in vivo have not been evaluated. This study aimed to examine the effects of crocin on HL-60 cells in vitro and in vivo and investigate the underlying mechanisms. HL-60 cells were treated by crocin, and cell proliferation, apoptosis, and cell cycle profiles were examined by MTT assay, AO/EB staining, and flow cytometry, respectively. Furthermore, HL-60 cells were xenografted into nude mice and treated by crocin, the tumor weight and size were calculated, and the expression of Bcl-2 and Bax in xenografts was detected by immunohistochemical staining. The results showed that crocin (0.625-5 mg/mL) inhibited HL-60 cell proliferation and induced apoptosis and cell cycle arrest at G0/G1 phase, in a concentration and time-dependent manner. In addition, crocin (6.25,25 mg/kg) inhibited the tumor weight and size of HL-60 xenografts in nude mice, inhibited Bcl-2 expression, and increased Bax expression in xenografts. In summary, crocin inhibits the proliferation and tumorigenicity of HL-60 cells, which may be mediated by the induction of apoptosis and cell cycle arrest and the regulation of Bcl-2 and Bax expression. [ABSTRACT FROM AUTHOR]

: Copyright of Evidence-based Complementary & Alternative Medicine (eCAM) is the property of Hindawi Limited and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Wen-Wu Bai, Yi-Fan Xing, Bo Wang, Xiao-Ting Lu, Ying-Bin Wang, Yuan-Yuan Sun, Xiao-Qiong Liu, Tao Guo, Yu-Xia Zhao

المصدر: Evidence-based Complementary & Alternative Medicine (eCAM); 2013, Vol. 2013, p1-10, 10p, 7 Graphs

مصطلحات موضوعية: ANALYSIS of variance, ANIMAL experimentation, APOPTOSIS, BIOLOGICAL models, HEART, HERBAL medicine, CHINESE medicine, MICE, MYOCARDIAL infarction, NEOVASCULARIZATION, RESEARCH funding, WESTERN immunoblotting, DATA analysis software

مستخلص: Background. Myocardial infarction (MI) is amajor cause of morbidity and mortality in the world. Tongxinluo (TXL) is a traditional Chinese compound prescription which has cardioprotective functions. The present study was aimed to determine the effect of TXL on postischemic cardiac dysfunction and cardiac remodeling and to elucidate the underlying mechanisms. Methods and Results. MI was performed by ligation of left anterior descending coronary artery (LAD) in male adult mice. Mice were randomly divided into four groups: (1) sham group (Sham); (2) MI-control group (Control); (3) MI-low dose TXL group (TXLL); and (4) MI-high dose TXL (TXL-H) group. Compared with the control group, TXL treatment restored cardiac function, increased revascularization, attenuated cardiomyocyte apoptosis, and reduced interstitial fibrosis. TXL treatment increased the phosphorylation of Akt, extracellular signal regulated kinase (ERK), and endothelial nitric oxide synthase (eNOS); the expression of phosphatidylinositol3-kinase (PI3K), hypoxia-inducible factors 1α (HIF-1α), and vascular endothelial growth factor (VEGF); and the DNA binding activity of HIF-1α after MI. Conclusion. TXL may improve cardiac function and ameliorate cardiac remodeling by increasing neovascularization through enhancing the phosphorylation of Akt and ERK, the expression and activity of HIF-1α, and the protein level of VEGF and p-eNOS. [ABSTRACT FROM AUTHOR]

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المؤلفون: Shuang-Xia Zhao, Chun-Ming Pan, Huang-Ming Cao, Bing Han, Jing-Yi Shi, Jun Liang, Guan-Qi Gao, Yong-De Peng, Qing Su, Jia-Lun Chen, Jia-Jun Zhao, Huai-Dong Song

المصدر: PLoS ONE; 2010, Vol. 5 Issue 3, p1-10, 10p

مصطلحات موضوعية: GRAVES' disease, REGRESSION analysis, THYROID eye disease, AUTOIMMUNE diseases, ANALYSIS of variance, LOGISTIC regression analysis, GENETIC polymorphisms, GENETIC research, POPULATION

مستخلص: To determine whether genetic heterogeneity exists in patients with Graves' disease (GD), the cytotoxic T-lymphocyte associated 4 (CTLA-4) gene, which is implicated a susceptibility gene for GD by considerable genetic and immunological evidence, was used for association analysis in a Chinese Han cohort recruited from various geographic regions. Our association study for the SNPs in the CTLA4 gene in 2640 GD patients and 2204 control subjects confirmed that CTLA4 is the susceptibility gene for GD in the Chinese Han population. Moreover, the logistic regression analysis in the combined Chinese Han cohort revealed that SNP rs231779 (allele frequencies p = 2.81610^-9, OR = 1.35, and genotype distributions p = 2.75610^-9, OR = 1.42) is likely the susceptibility variant for GD. Interestingly, the logistic regression analysis revealed that SNP rs35219727 may be the susceptibility variant to GD in the Shandong population; however, SNP, rs231779 in the CTLA4 gene probably independently confers GD susceptibility in the Xuzhou and southern China populations. These data suggest that the susceptibility variants of the CTLA4 gene varied between the different geographic populations with GD. [ABSTRACT FROM AUTHOR]

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المؤلفون: An-Chang Liu, Li-Xia Zhao, Hong-Xiang Lou

المصدر: Planta Medica; Jul2013, Vol. 79 Issue 11, p971-977, 7p

مصطلحات موضوعية: ANALYSIS of variance, ANIMAL experimentation, BLOOD coagulation, BLOOD platelet aggregation, LIQUID chromatography, MASS spectrometry, MOLECULAR structure, RATS, RESEARCH funding, STATISTICS, WARFARIN, DATA analysis, CLOPIDOGREL, DESCRIPTIVE statistics, CURCUMIN

مستخلص: This study examined the effects of curcumin on the pharmacokinetic and pharmacodynamic properties of warfarin and clopidogrel in Wistar rats. Results showed that oral administration of curcumin at 25mg/kg, 50mg/kg, and 100mg/kg for 7 days had no substantial effects on the pharmacodynamics of warfarin and clopidogrel in this animal model. However, oral administration of 100mg/kg curcumin for 7 days significantly increased the AUC∞-8 and Cmax of the two drugs (by x 1.6 and x 1.5, respectively, for warfarin, and x 1.61 and x 1.81, respectively, for clopidogrel carboxylic acid). However, compared to warfarin alone, different doses of curcumin combined with warfarin had no effects on the prothrombin time in rats. Similarly, a combination of curcumin and clopidogrel had no significant effect on the maximum platelet aggregation rate of rats compared with the use of clopidogrel alone. This work demonstrated that preadministration of 100mg/kg curcumin affected the pharmacokinetics of warfarin and clopidogrel but had no effect on pharmacodynamic parameters such as anticoagulation rate and antiplatelet aggregation. [ABSTRACT FROM AUTHOR]

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