التفاصيل البيبلوغرافية
| العنوان: |
铁死亡参与高蛋氨酸饮食所致的彳poE, 小鼠肾脏损伤. (Chinese) |
| العنوان البديل: |
Ferroptosis participates in renal damage induced by high methionine diet in ApoE-/- mice. (English) |
| المؤلفون: |
畅思容, 孙岳巴, 杨安宁, 李媛媛, 巴王梦, 宝瑞, 余梦雪, 刘志宏 |
| المصدر: |
Modern Preventive Medicine; 2023, Issue 17, p3133-3150, 7p |
| مصطلحات موضوعية: |
GLUTAMATE transporters, APOLIPOPROTEIN E, P53 protein, RENAL fibrosis, GENE expression |
| الملخص (بالإنجليزية): |
Objective To investigate whether ferroptosis is involved in renal damage in Apo E~f~ mice induced by high methionine diet. Methods C57BL/6J mice fed with normal diet were used as normal control group, ApoE, mice were randomly divided into model control group (fed with normal diet) and high methionine group (fed with high methionine diet), with 6 mice in each group. Renal injury and fibrosis in mice were observed by PAS and Masson staining. Detection of Fibronectin content by immunofluorescence staining and iron content was measured by colorimetry in renal tissue. The content of malondialdehyde (MDA) in renal tissue was detected by immunofluorescence staining and Thiobarbituric acid (TBA) method. Protein and mRNA expression of p53, SLC7A11, and GPX4 were determined by Western blotting and qRT-PCR. One -way ANOVA was used to compare multiple groups, and Tukey test was used for multiple comparisons. Results The staining results showed that apolipoprotein E (ApoE) gene deletion caused renal extracellular matrix deposition and renal injury, while high methionine diet further aggravated renal extracellular matrix deposition and renal injury. Compared with the normal control group, the content of Fibronectin in the model control group and high methionine group increased (於87.330, P<0.001), the content of iron ion (F=l 11.200, P<0.001) and MDA (7=205.200, P<0»001) increased, the protein level of p53 increased 0=32.380; P<0,001), the level of mRNA increased (F=24.840, P<0.001), the protein level of SLC7A11 and GPX4 decreased (slc7all=18.620, P<0,001; 74=20.830, P<0.001), and mRNA level decreased (码皿口=11200; P<0,001; F(赵4=23.530, P< 0.001). Compared with the model control groups the above indexes changed more significantly in the high methionine group (P<0.05). Conclusion Ferroptosis is involved in renal damage in Apo E~l~ mice induced by high methionine diet. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的 探讨铁死亡是否参与高蛋氨酸饮食所致的心。旷小鼠肾脏损伤。方法C57BU6J普通饲料喂养小鼠作为 正常对照组、将心。旷小鼠随机分为模型对照组(普通饲料喂养)和高蛋氨酸组筒蛋氨酸饲料喂养), 每组6只小鼠。PAS 和Masson染色观察小鼠肾脏损伤以及纤维化情况;免疫荧光染色检测肾组织纤维连接蛋白(fibronectin)含量;比色法检 测肾组织二价铁离子含量;免疫荧光染色和硫代巴比妥酸(tliiobarbituric acid, TBA)法检测肾组织丙二醛(malondialdehyde, MDA)#*;Western blotting和qRT-PCR检测p53、SLC7All和GPX4的蛋白以及mRNA表达水平,多组数据比较 采用单因素方差分析, 多重比较采用Tukey检验。结果 染色结果显示, 载脂蛋白E(apolipoprotein E, ApoE)因缺失引 起肾脏细胞外基质沉积, 出现肾损伤, 而高蛋氨酸饮食则进一步加重了肾脏细胞外基质沉积,肾损伤加重;与正常对照 组相比, 模型对照组和高蛋氨酸组Fibronectin含量增加(&87.330, Pv0.001),铁离子(F= 111.200,0.001)和MDA (f =205.200,P<0.001)含量增加, p53 的蛋白水平增加(F=32.380, Pv0.001)、mRNA 水平增加(F=24.840, Pv0.001), SLC7A11 和 GPX4 的蛋白水平降低(殛如=18.620, PvO.001;&齡=20.830, Pv0.001)、mRNA 水平降低(殛如=11.200, Pv 0.001; Fc妒23.530, PvO.001);与模型对照组相比, 以上指标在高蛋氨酸组变化更显著(Pv0.05)。结论铁死亡参与了 高蛋氨酸饮食所致的小鼠肾脏损伤. [ABSTRACT FROM AUTHOR] |
|
Copyright of Modern Preventive Medicine is the property of Modern Preventive Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
